Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles
Poly(<span style="font-variant: small-caps">d</span>,<span style="font-variant: small-caps">l</span>–lactide–co–glycolide) (PLGA) has been extensively explored for bone regeneration applications; however, its clinical use is limited by low osteointegration...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/10/3/33 |
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| author | Stuart C. Thickett Ella Hamilton Gokulan Yogeswaran Per B. Zetterlund Brooke L. Farrugia Megan S. Lord |
| author_facet | Stuart C. Thickett Ella Hamilton Gokulan Yogeswaran Per B. Zetterlund Brooke L. Farrugia Megan S. Lord |
| author_sort | Stuart C. Thickett |
| collection | DOAJ |
| description | Poly(<span style="font-variant: small-caps">d</span>,<span style="font-variant: small-caps">l</span>–lactide–co–glycolide) (PLGA) has been extensively explored for bone regeneration applications; however, its clinical use is limited by low osteointegration. Therefore, approaches that incorporate osteoconductive molecules are of great interest. Graphene oxide (GO) is gaining popularity for biomedical applications due to its ability to bind biological molecules and present them for enhanced bioactivity. This study reports the preparation of PLGA microparticles via Pickering emulsification using GO as the sole surfactant, which resulted in hybrid microparticles in the size range of 1.1 to 2.4 µm based on the ratio of GO to PLGA in the reaction. Furthermore, this study demonstrated that the hybrid GO-PLGA microparticles were not cytotoxic to either primary human fetal cartilage rudiment cells or the human osteoblast-like cell line, Saos-2. Additionally, the GO-PLGA microparticles promoted the osteogenic differentiation of the human fetal cartilage rudiment cells in the absence of exogenous growth factors to a greater extent than PLGA alone. These findings demonstrate that GO-PLGA microparticles are cytocompatible, osteoinductive and have potential as substrates for bone tissue engineering. |
| first_indexed | 2024-04-13T08:06:55Z |
| format | Article |
| id | doaj.art-6d5211ac10584159b7f24f999542c2cf |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-13T08:06:55Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-6d5211ac10584159b7f24f999542c2cf2022-12-22T02:55:08ZengMDPI AGJournal of Functional Biomaterials2079-49832019-07-011033310.3390/jfb10030033jfb10030033Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid MicroparticlesStuart C. Thickett0Ella Hamilton1Gokulan Yogeswaran2Per B. Zetterlund3Brooke L. Farrugia4Megan S. Lord5School of Natural Sciences (Chemistry), University of Tasmania, Hobart, TAS 7001, AustraliaGraduate School of Biomedical Engineering, UNSW Sydney, Sydney, NSW 2052, AustraliaGraduate School of Biomedical Engineering, UNSW Sydney, Sydney, NSW 2052, AustraliaCentre for Advanced Macromolecular Design, School of Chemical Engineering, UNSW Sydney, Sydney, NSW 2052, AustraliaGraduate School of Biomedical Engineering, UNSW Sydney, Sydney, NSW 2052, AustraliaGraduate School of Biomedical Engineering, UNSW Sydney, Sydney, NSW 2052, AustraliaPoly(<span style="font-variant: small-caps">d</span>,<span style="font-variant: small-caps">l</span>–lactide–co–glycolide) (PLGA) has been extensively explored for bone regeneration applications; however, its clinical use is limited by low osteointegration. Therefore, approaches that incorporate osteoconductive molecules are of great interest. Graphene oxide (GO) is gaining popularity for biomedical applications due to its ability to bind biological molecules and present them for enhanced bioactivity. This study reports the preparation of PLGA microparticles via Pickering emulsification using GO as the sole surfactant, which resulted in hybrid microparticles in the size range of 1.1 to 2.4 µm based on the ratio of GO to PLGA in the reaction. Furthermore, this study demonstrated that the hybrid GO-PLGA microparticles were not cytotoxic to either primary human fetal cartilage rudiment cells or the human osteoblast-like cell line, Saos-2. Additionally, the GO-PLGA microparticles promoted the osteogenic differentiation of the human fetal cartilage rudiment cells in the absence of exogenous growth factors to a greater extent than PLGA alone. These findings demonstrate that GO-PLGA microparticles are cytocompatible, osteoinductive and have potential as substrates for bone tissue engineering.https://www.mdpi.com/2079-4983/10/3/33PLGAgraphene oxideosteoblaststem cellbone tissue engineering |
| spellingShingle | Stuart C. Thickett Ella Hamilton Gokulan Yogeswaran Per B. Zetterlund Brooke L. Farrugia Megan S. Lord Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles Journal of Functional Biomaterials PLGA graphene oxide osteoblast stem cell bone tissue engineering |
| title | Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles |
| title_full | Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles |
| title_fullStr | Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles |
| title_full_unstemmed | Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles |
| title_short | Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles |
| title_sort | enhanced osteogenic differentiation of human fetal cartilage rudiment cells on graphene oxide plga hybrid microparticles |
| topic | PLGA graphene oxide osteoblast stem cell bone tissue engineering |
| url | https://www.mdpi.com/2079-4983/10/3/33 |
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