Fermentation Optimization, Fungistatic Effects and Tomato Growth Promotion of Four Biocontrol Bacterial Strains
Tomato is a widely cultivated crop that is important for its nutritional value and genetic diversity. Tomato production is seriously challenged by pests and diseases, among which tomato gray mold and leaf mold are particularly serious. Biological control is one of the most preferred methods for dise...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-07-01
|
Series: | Agriculture |
Subjects: | |
Online Access: | https://www.mdpi.com/2077-0472/11/7/686 |
_version_ | 1797527798061989888 |
---|---|
author | Yao Zhang Xingyuan Wang Sibo Liang Yuying Shi Xiuling Chen Jiayin Liu Aoxue Wang |
author_facet | Yao Zhang Xingyuan Wang Sibo Liang Yuying Shi Xiuling Chen Jiayin Liu Aoxue Wang |
author_sort | Yao Zhang |
collection | DOAJ |
description | Tomato is a widely cultivated crop that is important for its nutritional value and genetic diversity. Tomato production is seriously challenged by pests and diseases, among which tomato gray mold and leaf mold are particularly serious. Biological control is one of the most preferred methods for disease management in tomato production. At present, the fungi used to control tomato gray mold are mainly Trichoderma and yeast. <i>Bacillus</i> and actinomycetes are the most effective microorganisms for controlling tomato leaf mold. Tomato gray mold and leaf mold often occur at the same time during the production process, yet there are fewer strains for controlling both diseases at the same time. Biocontrol bacteria <i>Pseudomonas azotoformans</i> WXCDD51, <i>Bacillus</i> sp. WXCDD105, <i>Bacillus subtilis</i> BS and <i>Bacillus amyloliquefaciens</i> BS WY-1, which were isolated and screened in the previous stage, can prevent both tomato gray mold and leaf mold. Here, we optimized liquid fermentation for the four biocontrol bacterial strains together. We obtained the best fermentation medium formula and fermentation conditions for the four biocontrol bacteria. The broad-spectrum properties of the four biocontrol bacteria were tested, and, on this basis, compound strains were constructed. The control effect of single and compound strains on tomato gray mold and leaf mold was evaluated. Their potential effects on the growth of tomato seeds and seedlings were also studied. This research provides a foundation for the development and use of compound bacteria for growth promotion and disease management in tomato production. |
first_indexed | 2024-03-10T09:48:52Z |
format | Article |
id | doaj.art-6d524949b79d4389a83abf995b666b38 |
institution | Directory Open Access Journal |
issn | 2077-0472 |
language | English |
last_indexed | 2024-03-10T09:48:52Z |
publishDate | 2021-07-01 |
publisher | MDPI AG |
record_format | Article |
series | Agriculture |
spelling | doaj.art-6d524949b79d4389a83abf995b666b382023-11-22T02:57:44ZengMDPI AGAgriculture2077-04722021-07-0111768610.3390/agriculture11070686Fermentation Optimization, Fungistatic Effects and Tomato Growth Promotion of Four Biocontrol Bacterial StrainsYao Zhang0Xingyuan Wang1Sibo Liang2Yuying Shi3Xiuling Chen4Jiayin Liu5Aoxue Wang6College of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Sciences, Northeast Agricultural University, Harbin 150030, ChinaTomato is a widely cultivated crop that is important for its nutritional value and genetic diversity. Tomato production is seriously challenged by pests and diseases, among which tomato gray mold and leaf mold are particularly serious. Biological control is one of the most preferred methods for disease management in tomato production. At present, the fungi used to control tomato gray mold are mainly Trichoderma and yeast. <i>Bacillus</i> and actinomycetes are the most effective microorganisms for controlling tomato leaf mold. Tomato gray mold and leaf mold often occur at the same time during the production process, yet there are fewer strains for controlling both diseases at the same time. Biocontrol bacteria <i>Pseudomonas azotoformans</i> WXCDD51, <i>Bacillus</i> sp. WXCDD105, <i>Bacillus subtilis</i> BS and <i>Bacillus amyloliquefaciens</i> BS WY-1, which were isolated and screened in the previous stage, can prevent both tomato gray mold and leaf mold. Here, we optimized liquid fermentation for the four biocontrol bacterial strains together. We obtained the best fermentation medium formula and fermentation conditions for the four biocontrol bacteria. The broad-spectrum properties of the four biocontrol bacteria were tested, and, on this basis, compound strains were constructed. The control effect of single and compound strains on tomato gray mold and leaf mold was evaluated. Their potential effects on the growth of tomato seeds and seedlings were also studied. This research provides a foundation for the development and use of compound bacteria for growth promotion and disease management in tomato production.https://www.mdpi.com/2077-0472/11/7/686tomato gray moldtomato leaf moldbiocontrol bacterialiquid fermentationgrowth promotion |
spellingShingle | Yao Zhang Xingyuan Wang Sibo Liang Yuying Shi Xiuling Chen Jiayin Liu Aoxue Wang Fermentation Optimization, Fungistatic Effects and Tomato Growth Promotion of Four Biocontrol Bacterial Strains Agriculture tomato gray mold tomato leaf mold biocontrol bacteria liquid fermentation growth promotion |
title | Fermentation Optimization, Fungistatic Effects and Tomato Growth Promotion of Four Biocontrol Bacterial Strains |
title_full | Fermentation Optimization, Fungistatic Effects and Tomato Growth Promotion of Four Biocontrol Bacterial Strains |
title_fullStr | Fermentation Optimization, Fungistatic Effects and Tomato Growth Promotion of Four Biocontrol Bacterial Strains |
title_full_unstemmed | Fermentation Optimization, Fungistatic Effects and Tomato Growth Promotion of Four Biocontrol Bacterial Strains |
title_short | Fermentation Optimization, Fungistatic Effects and Tomato Growth Promotion of Four Biocontrol Bacterial Strains |
title_sort | fermentation optimization fungistatic effects and tomato growth promotion of four biocontrol bacterial strains |
topic | tomato gray mold tomato leaf mold biocontrol bacteria liquid fermentation growth promotion |
url | https://www.mdpi.com/2077-0472/11/7/686 |
work_keys_str_mv | AT yaozhang fermentationoptimizationfungistaticeffectsandtomatogrowthpromotionoffourbiocontrolbacterialstrains AT xingyuanwang fermentationoptimizationfungistaticeffectsandtomatogrowthpromotionoffourbiocontrolbacterialstrains AT siboliang fermentationoptimizationfungistaticeffectsandtomatogrowthpromotionoffourbiocontrolbacterialstrains AT yuyingshi fermentationoptimizationfungistaticeffectsandtomatogrowthpromotionoffourbiocontrolbacterialstrains AT xiulingchen fermentationoptimizationfungistaticeffectsandtomatogrowthpromotionoffourbiocontrolbacterialstrains AT jiayinliu fermentationoptimizationfungistaticeffectsandtomatogrowthpromotionoffourbiocontrolbacterialstrains AT aoxuewang fermentationoptimizationfungistaticeffectsandtomatogrowthpromotionoffourbiocontrolbacterialstrains |