Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway
Abstract Background Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the functi...
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-06-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-020-01754-z |
| _version_ | 1819198983359168512 |
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| author | Zheng-liang Shi Hua Zhang Zhi-yong Fan Wei Ma Yong-zhou Song Ming Li Tong-qiu Li Shu-xing Cao Guo-jun Feng |
| author_facet | Zheng-liang Shi Hua Zhang Zhi-yong Fan Wei Ma Yong-zhou Song Ming Li Tong-qiu Li Shu-xing Cao Guo-jun Feng |
| author_sort | Zheng-liang Shi |
| collection | DOAJ |
| description | Abstract Background Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. Methods LncRNA and miRNA sequencing was performed in normal and osteogenic differentiation-induced ADSCs (osteogenic group). Abnormally expressed lncRNAs and miRNAs were obtained by the R software and the relative expression of LINC00314, miR-129-5p, and GRM5 during osteogenic induction was measured by RT-PCR. ADSCs were then transfected with pcDNA3.1-sh-LINC00314 and agomiR-129-5p. Alizarin red staining (ARS) and alkaline phosphatase (ALP) staining were performed to identify the mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. Results LINC00314 was significantly upregulated in the group of osteogenic-induced ADSCs. LINC00314 and GRM5 mimics increased the early and late markers of osteogenic differentiation, which manifest in not only the markedly increased ALP activity but also higher calcium deposition, while miR-129-5p mimic had the opposite effects. LINC00314 directly targeted miR-129-5p through luciferase reporter assay, and miR-129-5p suppressed GRM5 expression. Moreover, the LINC00314/miR-129-5p/GRM5 regulatory axis activated the Wnt/β-catenin signaling pathway. Conclusions LINC00314 confers contributory function in the osteogenic differentiation of ADSCs and thus the LINC00314/miR-129-5p/GRM5 axis may be a novel mechanism for osteogenic-related disease. |
| first_indexed | 2024-12-23T03:09:06Z |
| format | Article |
| id | doaj.art-6d550565d16845579032e990d61dbdb3 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-23T03:09:06Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-6d550565d16845579032e990d61dbdb32022-12-21T18:02:15ZengBMCStem Cell Research & Therapy1757-65122020-06-0111111410.1186/s13287-020-01754-zLong noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathwayZheng-liang Shi0Hua Zhang1Zhi-yong Fan2Wei Ma3Yong-zhou Song4Ming Li5Tong-qiu Li6Shu-xing Cao7Guo-jun Feng8Department of Orthopedics, The Second Hospital of Hebei Medical UniversityDepartment of Orthopedics, The Second Hospital of Hebei Medical UniversityDepartment of Orthopedics, The Second Hospital of Hebei Medical UniversityDepartment of Orthopedics, The Second Hospital of Hebei Medical UniversityDepartment of Orthopedics, The Second Hospital of Hebei Medical UniversityDepartment of Orthopedics, The Second Hospital of Hebei Medical UniversityDepartment of Orthopedics, The Second Hospital of Hebei Medical UniversityDepartment of Orthopedics, The Second Hospital of Hebei Medical UniversityDepartment of Orthopedics, The Second Hospital of Hebei Medical UniversityAbstract Background Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. Methods LncRNA and miRNA sequencing was performed in normal and osteogenic differentiation-induced ADSCs (osteogenic group). Abnormally expressed lncRNAs and miRNAs were obtained by the R software and the relative expression of LINC00314, miR-129-5p, and GRM5 during osteogenic induction was measured by RT-PCR. ADSCs were then transfected with pcDNA3.1-sh-LINC00314 and agomiR-129-5p. Alizarin red staining (ARS) and alkaline phosphatase (ALP) staining were performed to identify the mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. Results LINC00314 was significantly upregulated in the group of osteogenic-induced ADSCs. LINC00314 and GRM5 mimics increased the early and late markers of osteogenic differentiation, which manifest in not only the markedly increased ALP activity but also higher calcium deposition, while miR-129-5p mimic had the opposite effects. LINC00314 directly targeted miR-129-5p through luciferase reporter assay, and miR-129-5p suppressed GRM5 expression. Moreover, the LINC00314/miR-129-5p/GRM5 regulatory axis activated the Wnt/β-catenin signaling pathway. Conclusions LINC00314 confers contributory function in the osteogenic differentiation of ADSCs and thus the LINC00314/miR-129-5p/GRM5 axis may be a novel mechanism for osteogenic-related disease.http://link.springer.com/article/10.1186/s13287-020-01754-zLINC00314miR-129-5pGRM5ADSCsOsteogenic differentiation |
| spellingShingle | Zheng-liang Shi Hua Zhang Zhi-yong Fan Wei Ma Yong-zhou Song Ming Li Tong-qiu Li Shu-xing Cao Guo-jun Feng Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway Stem Cell Research & Therapy LINC00314 miR-129-5p GRM5 ADSCs Osteogenic differentiation |
| title | Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway |
| title_full | Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway |
| title_fullStr | Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway |
| title_full_unstemmed | Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway |
| title_short | Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway |
| title_sort | long noncoding rna linc00314 facilitates osteogenic differentiation of adipose derived stem cells through the hsa mir 129 5p grm5 axis via the wnt signaling pathway |
| topic | LINC00314 miR-129-5p GRM5 ADSCs Osteogenic differentiation |
| url | http://link.springer.com/article/10.1186/s13287-020-01754-z |
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