Nodule Organogenesis and Symbiotic Mutants of the Model Legume Lotus japonicus
A detailed microscopical analysis of the morphological features that distinguish different developmental stages of nodule organogenesis in wild-type Lotus japonicus ecotype Gifu B-129-S9 plants was performed, to provide the necessary framework for the evaluation of altered phenotypes of L. japonicus...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1998-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.7.684 |
| _version_ | 1811264580124409856 |
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| author | Krzysztof Szczyglowski Robert S. Shaw Judith Wopereis Sue Copeland Dirk Hamburger Beth Kasiborski Frank B. Dazzo Frans J. de Bruijn |
| author_facet | Krzysztof Szczyglowski Robert S. Shaw Judith Wopereis Sue Copeland Dirk Hamburger Beth Kasiborski Frank B. Dazzo Frans J. de Bruijn |
| author_sort | Krzysztof Szczyglowski |
| collection | DOAJ |
| description | A detailed microscopical analysis of the morphological features that distinguish different developmental stages of nodule organogenesis in wild-type Lotus japonicus ecotype Gifu B-129-S9 plants was performed, to provide the necessary framework for the evaluation of altered phenotypes of L. japonicus symbiotic mutants. Subsequently, chemical ethyl methanesulfonate (EMS) mutagenesis of L. japonicus was carried out. The analysis of approximately 3,000 M1 plants and their progeny yielded 20 stable L. japonicus symbiotic variants, consisting of at least 14 different symbiosis-associated loci or complementation groups. Moreover, a mutation affecting L. japonicus root development was identified that also conferred a hypernodulation response when a line carrying the corresponding allele (LjEMS102) was inoculated with rhizobia. The phenotype of the LjEMS102 line was characterized by the presence of nodule structures covering almost the entire root length (Nod++), and by a concomitant inhibition of both root and stem growth. A mutation in a single nuclear gene was shown to be responsible for both root and symbiotic phenotypes observed in the L. japonicus LjEMS102 line, suggesting that (a) common mechanism(s) regulating root development and nodule formation exists in legumes. |
| first_indexed | 2024-04-12T20:06:30Z |
| format | Article |
| id | doaj.art-6d55c34e6e3149d3b6180244617beac6 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:06:30Z |
| publishDate | 1998-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-6d55c34e6e3149d3b6180244617beac62022-12-22T03:18:23ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061998-07-0111768469710.1094/MPMI.1998.11.7.684Nodule Organogenesis and Symbiotic Mutants of the Model Legume Lotus japonicusKrzysztof SzczyglowskiRobert S. ShawJudith WopereisSue CopelandDirk HamburgerBeth KasiborskiFrank B. DazzoFrans J. de BruijnA detailed microscopical analysis of the morphological features that distinguish different developmental stages of nodule organogenesis in wild-type Lotus japonicus ecotype Gifu B-129-S9 plants was performed, to provide the necessary framework for the evaluation of altered phenotypes of L. japonicus symbiotic mutants. Subsequently, chemical ethyl methanesulfonate (EMS) mutagenesis of L. japonicus was carried out. The analysis of approximately 3,000 M1 plants and their progeny yielded 20 stable L. japonicus symbiotic variants, consisting of at least 14 different symbiosis-associated loci or complementation groups. Moreover, a mutation affecting L. japonicus root development was identified that also conferred a hypernodulation response when a line carrying the corresponding allele (LjEMS102) was inoculated with rhizobia. The phenotype of the LjEMS102 line was characterized by the presence of nodule structures covering almost the entire root length (Nod++), and by a concomitant inhibition of both root and stem growth. A mutation in a single nuclear gene was shown to be responsible for both root and symbiotic phenotypes observed in the L. japonicus LjEMS102 line, suggesting that (a) common mechanism(s) regulating root development and nodule formation exists in legumes.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.7.684 |
| spellingShingle | Krzysztof Szczyglowski Robert S. Shaw Judith Wopereis Sue Copeland Dirk Hamburger Beth Kasiborski Frank B. Dazzo Frans J. de Bruijn Nodule Organogenesis and Symbiotic Mutants of the Model Legume Lotus japonicus Molecular Plant-Microbe Interactions |
| title | Nodule Organogenesis and Symbiotic Mutants of the Model Legume Lotus japonicus |
| title_full | Nodule Organogenesis and Symbiotic Mutants of the Model Legume Lotus japonicus |
| title_fullStr | Nodule Organogenesis and Symbiotic Mutants of the Model Legume Lotus japonicus |
| title_full_unstemmed | Nodule Organogenesis and Symbiotic Mutants of the Model Legume Lotus japonicus |
| title_short | Nodule Organogenesis and Symbiotic Mutants of the Model Legume Lotus japonicus |
| title_sort | nodule organogenesis and symbiotic mutants of the model legume lotus japonicus |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.7.684 |
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