Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria
Abstract Background The need to investigate the contribution of non-polio enteroviruses to acute flaccid paralysis (AFP) cannot be over emphasized as we move towards a poliovirus free world. Hence, we aim to identify non-polio enteroviruses recovered from the faeces of children diagnosed with AFP in...
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | Virology Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12985-017-0846-x |
| _version_ | 1828757565649977344 |
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| author | T. O. C. Faleye M. O. Adewumi M. O. Japhet O. M. David A. O. Oluyege J. A. Adeniji O. Famurewa |
| author_facet | T. O. C. Faleye M. O. Adewumi M. O. Japhet O. M. David A. O. Oluyege J. A. Adeniji O. Famurewa |
| author_sort | T. O. C. Faleye |
| collection | DOAJ |
| description | Abstract Background The need to investigate the contribution of non-polio enteroviruses to acute flaccid paralysis (AFP) cannot be over emphasized as we move towards a poliovirus free world. Hence, we aim to identify non-polio enteroviruses recovered from the faeces of children diagnosed with AFP in Nigeria. Methods Ninety-six isolates, (95 unidentified and one previously confirmed Sabin poliovirus 3) recovered on RD cell culture from the stool of children <15 years old diagnosed with AFP in 2014 were analyzed. All isolates were subjected to RNA extraction, cDNA synthesis and three different PCR reactions (one panenterovirus 5′-UTR and two different VP1 amplification assays). VP1 amplicons were then sequenced and isolates identified. Results 92.71% (89/96) of the isolates were detected by at least one of the three assays as an enterovirus. Precisely, 79.17% (76/96), 6.25% (6/96), 7.30% (7/96) and 7.30% (7/96) of the isolates were positive for both, positive and negative, negative and positive, as well as negative for both the 5′-UTR and VP1 assays, respectively. In this study, sixty-nine (69) of the 83 VP1 amplicons sequenced were identified as 27 different enterovirus types. The most commonly detected were CV-B3 (10 isolates) and EV-B75 (5 isolates). Specifically, one, twenty-four and two of the enterovirus types identified in this study belong to EV-A, EV-B and EV-C respectively. Conclusions This study reports the circulating strains of 27 non-polio enterovirus types in Nigerian children with AFP in 2014 and Nigerian strains of CV-B2, CV-B4, E17, EV-B80, EV-B73, EV-B97, EV-B93, EV-C99 and EV-A120 were reported for the first time. Furthermore, it shows that being positive for the 5′-UTR assay should not be the basis for subjecting isolates to the VP1 assays. |
| first_indexed | 2024-12-11T00:27:55Z |
| format | Article |
| id | doaj.art-6d57899aee604214829dccdbd5fb2151 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-11T00:27:55Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-6d57899aee604214829dccdbd5fb21512022-12-22T01:27:28ZengBMCVirology Journal1743-422X2017-09-0114111410.1186/s12985-017-0846-xNon-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in NigeriaT. O. C. Faleye0M. O. Adewumi1M. O. Japhet2O. M. David3A. O. Oluyege4J. A. Adeniji5O. Famurewa6Department of Microbiology, Faculty of Science, Ekiti State UniversityDepartment of Virology, College of Medicine, University of IbadanDepartment of Microbiology, Faculty of Science, Ekiti State UniversityDepartment of Microbiology, Faculty of Science, Ekiti State UniversityDepartment of Microbiology, Faculty of Science, Ekiti State UniversityDepartment of Virology, College of Medicine, University of IbadanDepartment of Microbiology, Faculty of Science, Ekiti State UniversityAbstract Background The need to investigate the contribution of non-polio enteroviruses to acute flaccid paralysis (AFP) cannot be over emphasized as we move towards a poliovirus free world. Hence, we aim to identify non-polio enteroviruses recovered from the faeces of children diagnosed with AFP in Nigeria. Methods Ninety-six isolates, (95 unidentified and one previously confirmed Sabin poliovirus 3) recovered on RD cell culture from the stool of children <15 years old diagnosed with AFP in 2014 were analyzed. All isolates were subjected to RNA extraction, cDNA synthesis and three different PCR reactions (one panenterovirus 5′-UTR and two different VP1 amplification assays). VP1 amplicons were then sequenced and isolates identified. Results 92.71% (89/96) of the isolates were detected by at least one of the three assays as an enterovirus. Precisely, 79.17% (76/96), 6.25% (6/96), 7.30% (7/96) and 7.30% (7/96) of the isolates were positive for both, positive and negative, negative and positive, as well as negative for both the 5′-UTR and VP1 assays, respectively. In this study, sixty-nine (69) of the 83 VP1 amplicons sequenced were identified as 27 different enterovirus types. The most commonly detected were CV-B3 (10 isolates) and EV-B75 (5 isolates). Specifically, one, twenty-four and two of the enterovirus types identified in this study belong to EV-A, EV-B and EV-C respectively. Conclusions This study reports the circulating strains of 27 non-polio enterovirus types in Nigerian children with AFP in 2014 and Nigerian strains of CV-B2, CV-B4, E17, EV-B80, EV-B73, EV-B97, EV-B93, EV-C99 and EV-A120 were reported for the first time. Furthermore, it shows that being positive for the 5′-UTR assay should not be the basis for subjecting isolates to the VP1 assays.http://link.springer.com/article/10.1186/s12985-017-0846-xAFPEnterovirusesNigeriaNon-polio enterovirusesVP1 analysis |
| spellingShingle | T. O. C. Faleye M. O. Adewumi M. O. Japhet O. M. David A. O. Oluyege J. A. Adeniji O. Famurewa Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria Virology Journal AFP Enteroviruses Nigeria Non-polio enteroviruses VP1 analysis |
| title | Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria |
| title_full | Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria |
| title_fullStr | Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria |
| title_full_unstemmed | Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria |
| title_short | Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria |
| title_sort | non polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in nigeria |
| topic | AFP Enteroviruses Nigeria Non-polio enteroviruses VP1 analysis |
| url | http://link.springer.com/article/10.1186/s12985-017-0846-x |
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