Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to <i>Pichia anomala</i> Induced with Chitosan
Transcriptome analysis (TA) was conducted to characterize the transcriptome changes in postharvest disease-related genes of table grapes following treatment with <i>Pichia anomala</i> induced with chitosan (1% <i>w</i>/<i>v</i>). In the current study, the differen...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/7/1451 |
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| author | Wanying Hu Esa Abiso Godana Meiqiu Xu Qiya Yang Solairaj Dhanasekaran Hongyin Zhang |
| author_facet | Wanying Hu Esa Abiso Godana Meiqiu Xu Qiya Yang Solairaj Dhanasekaran Hongyin Zhang |
| author_sort | Wanying Hu |
| collection | DOAJ |
| description | Transcriptome analysis (TA) was conducted to characterize the transcriptome changes in postharvest disease-related genes of table grapes following treatment with <i>Pichia anomala</i> induced with chitosan (1% <i>w</i>/<i>v</i>). In the current study, the difference in the gene expression of table grapes after treatment with <i>P. anomala</i> induced with chitosan and that of a control group was compared 72 h post-inoculation. The study revealed that postharvest treatment of table grapes with <i>P. anomala</i> induced with chitosan could up-regulate genes that have a pivotal role in the fruit’s disease defense. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) results also confirmed that GO terms and the KEGG pathways, which have pivotal roles in plant disease resistance, were significantly enriched. The up-regulated genes of the treatment group have a unique function in the fruit’s disease resistance compared to the control group. Generally, most genes in the plant–pathogen interaction pathway; the plant Mitogen-activated protein kinase (MAPK) signaling pathway; the plant hormone signal transduction pathway; the pathway of glutathione metabolism; the pathway of phenylalanine, tyrosine, and tryptophan biosynthesis; and the pathway of flavonoid biosynthesis were all up-regulated. These up-regulations help the fruit to synthesize disease-resistant substances, regulate the reactive oxygen species (ROS), enhance the fruit cell wall, and enrich hormone signal transduction during the pathogen’s attack. This study is useful to overcome the lags in applying transcriptomics technology in postharvest pathology, and will provide insight towards developing other alternative methods to using bio-pesticides to control postharvest diseases of perishables. |
| first_indexed | 2024-03-10T10:09:44Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T10:09:44Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-6d5997683431478a876f8edfbe1e71412023-11-22T01:15:49ZengMDPI AGFoods2304-81582021-06-01107145110.3390/foods10071451Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to <i>Pichia anomala</i> Induced with ChitosanWanying Hu0Esa Abiso Godana1Meiqiu Xu2Qiya Yang3Solairaj Dhanasekaran4Hongyin Zhang5School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaTranscriptome analysis (TA) was conducted to characterize the transcriptome changes in postharvest disease-related genes of table grapes following treatment with <i>Pichia anomala</i> induced with chitosan (1% <i>w</i>/<i>v</i>). In the current study, the difference in the gene expression of table grapes after treatment with <i>P. anomala</i> induced with chitosan and that of a control group was compared 72 h post-inoculation. The study revealed that postharvest treatment of table grapes with <i>P. anomala</i> induced with chitosan could up-regulate genes that have a pivotal role in the fruit’s disease defense. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) results also confirmed that GO terms and the KEGG pathways, which have pivotal roles in plant disease resistance, were significantly enriched. The up-regulated genes of the treatment group have a unique function in the fruit’s disease resistance compared to the control group. Generally, most genes in the plant–pathogen interaction pathway; the plant Mitogen-activated protein kinase (MAPK) signaling pathway; the plant hormone signal transduction pathway; the pathway of glutathione metabolism; the pathway of phenylalanine, tyrosine, and tryptophan biosynthesis; and the pathway of flavonoid biosynthesis were all up-regulated. These up-regulations help the fruit to synthesize disease-resistant substances, regulate the reactive oxygen species (ROS), enhance the fruit cell wall, and enrich hormone signal transduction during the pathogen’s attack. This study is useful to overcome the lags in applying transcriptomics technology in postharvest pathology, and will provide insight towards developing other alternative methods to using bio-pesticides to control postharvest diseases of perishables.https://www.mdpi.com/2304-8158/10/7/1451biological controldisease resistance<i>Pichia anomala</i>RNA sequencinggene ontologyKyoto Encyclopedia of Genes and Genomes (KEGG) |
| spellingShingle | Wanying Hu Esa Abiso Godana Meiqiu Xu Qiya Yang Solairaj Dhanasekaran Hongyin Zhang Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to <i>Pichia anomala</i> Induced with Chitosan Foods biological control disease resistance <i>Pichia anomala</i> RNA sequencing gene ontology Kyoto Encyclopedia of Genes and Genomes (KEGG) |
| title | Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to <i>Pichia anomala</i> Induced with Chitosan |
| title_full | Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to <i>Pichia anomala</i> Induced with Chitosan |
| title_fullStr | Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to <i>Pichia anomala</i> Induced with Chitosan |
| title_full_unstemmed | Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to <i>Pichia anomala</i> Induced with Chitosan |
| title_short | Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to <i>Pichia anomala</i> Induced with Chitosan |
| title_sort | transcriptome characterization and expression profiles of disease defense related genes of table grapes in response to i pichia anomala i induced with chitosan |
| topic | biological control disease resistance <i>Pichia anomala</i> RNA sequencing gene ontology Kyoto Encyclopedia of Genes and Genomes (KEGG) |
| url | https://www.mdpi.com/2304-8158/10/7/1451 |
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