Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring
Abstract Background Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as w...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-11-01
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| Series: | Journal of Biomedical Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12929-020-00694-3 |
| _version_ | 1818875096989696000 |
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| author | Maria García-Ricobaraza Mercedes García-Bermúdez Francisco J. Torres-Espinola M. Teresa Segura Moreno Mathieu N. Bleyere Ligia E. Díaz-Prieto Esther Nova Ascensión Marcos Cristina Campoy |
| author_facet | Maria García-Ricobaraza Mercedes García-Bermúdez Francisco J. Torres-Espinola M. Teresa Segura Moreno Mathieu N. Bleyere Ligia E. Díaz-Prieto Esther Nova Ascensión Marcos Cristina Campoy |
| author_sort | Maria García-Ricobaraza |
| collection | DOAJ |
| description | Abstract Background Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as well as the maturation of monocytes/macrophages and the control of inflammatory reactions. The aim of this study was to evaluate the relationship between the Pro12Ala (rs1808212) PPARG gene polymorphism on immune molecular and cellular components in mothers and their offspring participating in the PREOBE study. Methods DNA from maternal venous blood samples at 24, 34 and 40 gestational weeks, plus cord blood samples was extracted. Pro12Ala PPARG polymorphism genotyping was performed, and immune system markers were analyzed by flow cytometry. Results Study findings revealed no effect of rs1808212 PPARG genotypes on innate immune parameters in mothers and their offspring; however, CD4 + /CD8 + ratio were decreased at 24 and 34 weeks in pregnant women carrying the CG (Pro12Ala) rs1808212 polymorphism, (p = 0,012 and p = 0,030; respectively). Only CD19 levels in peripheral blood were significantly higher at delivery in pregnant women carrying the CC (Pro12Pro) genotype (p ≤ 0.001). Moreover, there were statistically significant differences in leukocytes and neutrophils maternal levels at 34 weeks of gestation, being lower in carriers of Pro12Ala genotype (p = 0.028 and p = 0.031, respectively). Conclusions Results suggest that Pro12Ala PPARG polymorphism may have an effect on some cell and immune parameters in pregnant women during pregnancy and at time of delivery. However, newborn innate immune system does not seems to be influenced by PPARG Pro12Ala polymorphism in cord blood. |
| first_indexed | 2024-12-19T13:21:04Z |
| format | Article |
| id | doaj.art-6d5deb790d384189b3b9e7861e9134b2 |
| institution | Directory Open Access Journal |
| issn | 1423-0127 |
| language | English |
| last_indexed | 2024-12-19T13:21:04Z |
| publishDate | 2020-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biomedical Science |
| spelling | doaj.art-6d5deb790d384189b3b9e7861e9134b22022-12-21T20:19:42ZengBMCJournal of Biomedical Science1423-01272020-11-012711910.1186/s12929-020-00694-3Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspringMaria García-Ricobaraza0Mercedes García-Bermúdez1Francisco J. Torres-Espinola2M. Teresa Segura Moreno3Mathieu N. Bleyere4Ligia E. Díaz-Prieto5Esther Nova6Ascensión Marcos7Cristina Campoy8Department of Paediatrics, School of Medicine, Universidad de GranadaDepartment of Paediatrics, School of Medicine, Universidad de GranadaDepartment of Paediatrics, School of Medicine, Universidad de GranadaDepartment of Paediatrics, School of Medicine, Universidad de GranadaDepartment of Physiology, Haematology and Immunology, Nangui Abrogoua UniversityInstitute of Food Science, Technology and Nutrition (ICTAN), CSICInstitute of Food Science, Technology and Nutrition (ICTAN), CSICInstitute of Food Science, Technology and Nutrition (ICTAN), CSICDepartment of Paediatrics, School of Medicine, Universidad de GranadaAbstract Background Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as well as the maturation of monocytes/macrophages and the control of inflammatory reactions. The aim of this study was to evaluate the relationship between the Pro12Ala (rs1808212) PPARG gene polymorphism on immune molecular and cellular components in mothers and their offspring participating in the PREOBE study. Methods DNA from maternal venous blood samples at 24, 34 and 40 gestational weeks, plus cord blood samples was extracted. Pro12Ala PPARG polymorphism genotyping was performed, and immune system markers were analyzed by flow cytometry. Results Study findings revealed no effect of rs1808212 PPARG genotypes on innate immune parameters in mothers and their offspring; however, CD4 + /CD8 + ratio were decreased at 24 and 34 weeks in pregnant women carrying the CG (Pro12Ala) rs1808212 polymorphism, (p = 0,012 and p = 0,030; respectively). Only CD19 levels in peripheral blood were significantly higher at delivery in pregnant women carrying the CC (Pro12Pro) genotype (p ≤ 0.001). Moreover, there were statistically significant differences in leukocytes and neutrophils maternal levels at 34 weeks of gestation, being lower in carriers of Pro12Ala genotype (p = 0.028 and p = 0.031, respectively). Conclusions Results suggest that Pro12Ala PPARG polymorphism may have an effect on some cell and immune parameters in pregnant women during pregnancy and at time of delivery. However, newborn innate immune system does not seems to be influenced by PPARG Pro12Ala polymorphism in cord blood.https://doi.org/10.1186/s12929-020-00694-3Pro12Ala PPARG polymorphismImmune systemPregnant mothersNewborn cord blood |
| spellingShingle | Maria García-Ricobaraza Mercedes García-Bermúdez Francisco J. Torres-Espinola M. Teresa Segura Moreno Mathieu N. Bleyere Ligia E. Díaz-Prieto Esther Nova Ascensión Marcos Cristina Campoy Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring Journal of Biomedical Science Pro12Ala PPARG polymorphism Immune system Pregnant mothers Newborn cord blood |
| title | Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring |
| title_full | Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring |
| title_fullStr | Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring |
| title_full_unstemmed | Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring |
| title_short | Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring |
| title_sort | association study of rs1801282 pparg gene polymorphism and immune cells and cytokine levels in a spanish pregnant women cohort and their offspring |
| topic | Pro12Ala PPARG polymorphism Immune system Pregnant mothers Newborn cord blood |
| url | https://doi.org/10.1186/s12929-020-00694-3 |
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