The insulin-degrading enzyme as a link between insulin and neuropeptides metabolism
We have applied a recently developed HPLC-MS enzymatic assay to investigate the cryptic peptides generated by the action of the insulin-degrading enzyme (IDE) on some neuropeptides (NPs) involved in the development of tolerance and dependence to opioids. Particularly, the tested NPs are generated fr...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-01-01
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| Series: | Journal of Enzyme Inhibition and Medicinal Chemistry |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/14756366.2020.1850712 |
| _version_ | 1818975621558042624 |
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| author | Dorota Wacławczyk Jerzy Silberring Giuseppe Grasso |
| author_facet | Dorota Wacławczyk Jerzy Silberring Giuseppe Grasso |
| author_sort | Dorota Wacławczyk |
| collection | DOAJ |
| description | We have applied a recently developed HPLC-MS enzymatic assay to investigate the cryptic peptides generated by the action of the insulin-degrading enzyme (IDE) on some neuropeptides (NPs) involved in the development of tolerance and dependence to opioids. Particularly, the tested NPs are generated from the NPFF precursor (pro-NPFF (A)): NPFF (FLFQPQRF) and NPAF (AGEGLSSPFWSLAAPQRF). The results show that IDE is able to cleave NPFF and NPAF, generating specific cryptic peptides. As IDE is also responsible for the processing of many other peptides in the brain (amyloid beta protein among the others), we have also performed competitive degradation assays using mixtures of insulin and the above mentioned NPs. Data show that insulin is able to slow down the degradation of both NPs tested, whereas, surprisingly, NPAF is able to accelerate insulin degradation, hinting IDE as the possible link responsible of the mutual influence between insulin and NPs metabolism. |
| first_indexed | 2024-12-20T15:58:52Z |
| format | Article |
| id | doaj.art-6d5f477dd101498794ff6e43560ece51 |
| institution | Directory Open Access Journal |
| issn | 1475-6366 1475-6374 |
| language | English |
| last_indexed | 2024-12-20T15:58:52Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Enzyme Inhibition and Medicinal Chemistry |
| spelling | doaj.art-6d5f477dd101498794ff6e43560ece512022-12-21T19:34:21ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742021-01-0136118318710.1080/14756366.2020.18507121850712The insulin-degrading enzyme as a link between insulin and neuropeptides metabolismDorota Wacławczyk0Jerzy Silberring1Giuseppe Grasso2Department of Biochemistry and Neurobiology, AGH University of Science and TechnologyDepartment of Biochemistry and Neurobiology, AGH University of Science and TechnologyDepartment of Chemical Sciences, University of CataniaWe have applied a recently developed HPLC-MS enzymatic assay to investigate the cryptic peptides generated by the action of the insulin-degrading enzyme (IDE) on some neuropeptides (NPs) involved in the development of tolerance and dependence to opioids. Particularly, the tested NPs are generated from the NPFF precursor (pro-NPFF (A)): NPFF (FLFQPQRF) and NPAF (AGEGLSSPFWSLAAPQRF). The results show that IDE is able to cleave NPFF and NPAF, generating specific cryptic peptides. As IDE is also responsible for the processing of many other peptides in the brain (amyloid beta protein among the others), we have also performed competitive degradation assays using mixtures of insulin and the above mentioned NPs. Data show that insulin is able to slow down the degradation of both NPs tested, whereas, surprisingly, NPAF is able to accelerate insulin degradation, hinting IDE as the possible link responsible of the mutual influence between insulin and NPs metabolism.http://dx.doi.org/10.1080/14756366.2020.1850712insulin-degrading enzymeneuropeptidesdiabetesopiods |
| spellingShingle | Dorota Wacławczyk Jerzy Silberring Giuseppe Grasso The insulin-degrading enzyme as a link between insulin and neuropeptides metabolism Journal of Enzyme Inhibition and Medicinal Chemistry insulin-degrading enzyme neuropeptides diabetes opiods |
| title | The insulin-degrading enzyme as a link between insulin and neuropeptides metabolism |
| title_full | The insulin-degrading enzyme as a link between insulin and neuropeptides metabolism |
| title_fullStr | The insulin-degrading enzyme as a link between insulin and neuropeptides metabolism |
| title_full_unstemmed | The insulin-degrading enzyme as a link between insulin and neuropeptides metabolism |
| title_short | The insulin-degrading enzyme as a link between insulin and neuropeptides metabolism |
| title_sort | insulin degrading enzyme as a link between insulin and neuropeptides metabolism |
| topic | insulin-degrading enzyme neuropeptides diabetes opiods |
| url | http://dx.doi.org/10.1080/14756366.2020.1850712 |
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