Using the Volta phase plate with defocus for cryo-EM single particle analysis

Using the Volta phase plate with defocus for cryo-EM single particle analysis

Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybri...

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Bibliographic Details
Main Authors: Radostin Danev, Dimitry Tegunov, Wolfgang Baumeister
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-01-01
Series:eLife
Subjects:
phase plate
cryo-EM
proteasome
Online Access:https://elifesciences.org/articles/23006

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