Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
Abstract Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection. However, whether Chlamydia trachomatis has a negative impact on sperm quality and male fertility is still controversial. Herein, we report the effects on sperm quality of the in vitro exposure of spermatoz...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-01262-w |
| _version_ | 1818851042576564224 |
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| author | Jenniffer Puerta Suarez Leonardo R. Sanchez Florencia C. Salazar Hector A. Saka Rosa Molina Andrea Tissera Virginia E. Rivero Walter D. Cardona Maya Ruben D. Motrich |
| author_facet | Jenniffer Puerta Suarez Leonardo R. Sanchez Florencia C. Salazar Hector A. Saka Rosa Molina Andrea Tissera Virginia E. Rivero Walter D. Cardona Maya Ruben D. Motrich |
| author_sort | Jenniffer Puerta Suarez |
| collection | DOAJ |
| description | Abstract Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection. However, whether Chlamydia trachomatis has a negative impact on sperm quality and male fertility is still controversial. Herein, we report the effects on sperm quality of the in vitro exposure of spermatozoa to Chlamydia trachomatis, and also the effects of male genital infection on male fertility using an animal model. Human and mouse sperm were obtained from healthy donors and cauda epididimys from C57BL/6 mice, respectively. Highly motile human or mouse spermatozoa were in vitro exposed to C. trachomatis (serovar E or LGV) or C. muridarum, respectively. Then, sperm quality parameters were analyzed. Moreover, male fertility of Chlamydia muridarum infected male C57BL/6 mice was assessed. Human or murine sperm in vitro exposed to increasing bacterial concentrations or soluble factors from C. trachomatis or C. muridarum, respectively, did not show differences in sperm motility and viability, apoptosis, mitochondrial membrane potential, DNA fragmentation, ROS production and lipid peroxidation levels, when compared with control sperm (p > 0.05). Moreover, no differences in fertility parameters (potency, fecundity, fertility index, pre- and post-implantation loss) were observed between control and infected males. In conclusion, our results indicate that Chlamydia spp. neither directly exerts deleterious effects on spermatozoa nor impairs male fertility. |
| first_indexed | 2024-12-19T06:58:44Z |
| format | Article |
| id | doaj.art-6d61bf49b78e40db8de3cc82ca97f355 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T06:58:44Z |
| publishDate | 2017-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6d61bf49b78e40db8de3cc82ca97f3552022-12-21T20:31:27ZengNature PortfolioScientific Reports2045-23222017-04-017111410.1038/s41598-017-01262-wChlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertilityJenniffer Puerta Suarez0Leonardo R. Sanchez1Florencia C. Salazar2Hector A. Saka3Rosa Molina4Andrea Tissera5Virginia E. Rivero6Walter D. Cardona Maya7Ruben D. Motrich8Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Departamento de Bioquímica Clínica. Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina AllendeCentro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Departamento de Bioquímica Clínica. Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina AllendeCentro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Departamento de Bioquímica Clínica. Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina AllendeCentro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Departamento de Bioquímica Clínica. Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina AllendeLaboratorio de Andrología y ReproducciónLaboratorio de Andrología y ReproducciónCentro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Departamento de Bioquímica Clínica. Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina AllendeGrupo Reproducción, Facultad de Medicina, Universidad de Antioquia, Sede de Investigación Universitaria, SIUCentro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Departamento de Bioquímica Clínica. Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina AllendeAbstract Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection. However, whether Chlamydia trachomatis has a negative impact on sperm quality and male fertility is still controversial. Herein, we report the effects on sperm quality of the in vitro exposure of spermatozoa to Chlamydia trachomatis, and also the effects of male genital infection on male fertility using an animal model. Human and mouse sperm were obtained from healthy donors and cauda epididimys from C57BL/6 mice, respectively. Highly motile human or mouse spermatozoa were in vitro exposed to C. trachomatis (serovar E or LGV) or C. muridarum, respectively. Then, sperm quality parameters were analyzed. Moreover, male fertility of Chlamydia muridarum infected male C57BL/6 mice was assessed. Human or murine sperm in vitro exposed to increasing bacterial concentrations or soluble factors from C. trachomatis or C. muridarum, respectively, did not show differences in sperm motility and viability, apoptosis, mitochondrial membrane potential, DNA fragmentation, ROS production and lipid peroxidation levels, when compared with control sperm (p > 0.05). Moreover, no differences in fertility parameters (potency, fecundity, fertility index, pre- and post-implantation loss) were observed between control and infected males. In conclusion, our results indicate that Chlamydia spp. neither directly exerts deleterious effects on spermatozoa nor impairs male fertility.https://doi.org/10.1038/s41598-017-01262-w |
| spellingShingle | Jenniffer Puerta Suarez Leonardo R. Sanchez Florencia C. Salazar Hector A. Saka Rosa Molina Andrea Tissera Virginia E. Rivero Walter D. Cardona Maya Ruben D. Motrich Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility Scientific Reports |
| title | Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility |
| title_full | Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility |
| title_fullStr | Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility |
| title_full_unstemmed | Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility |
| title_short | Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility |
| title_sort | chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility |
| url | https://doi.org/10.1038/s41598-017-01262-w |
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