Evaluation of the Hematological and Serum Biochemistry Parameters in the Pre-Symptomatic and Symptomatic Stages of ALS Disease to Support Early Diagnosis and Prognosis
Amyotrophic lateral sclerosis (ALS) is the most common motor neuron disease. Since there are no pathognomonic tests for ALS prognoses; clinical diagnoses of the disease take time and are usually difficult. Prognostic biomarkers are urgently needed for rapid and effective ALS prognoses. Male albino r...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/22/3569 |
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| author | Duygu Aydemir Selcuk Surucu Ayse Nazli Basak Nuriye Nuray Ulusu |
| author_facet | Duygu Aydemir Selcuk Surucu Ayse Nazli Basak Nuriye Nuray Ulusu |
| author_sort | Duygu Aydemir |
| collection | DOAJ |
| description | Amyotrophic lateral sclerosis (ALS) is the most common motor neuron disease. Since there are no pathognomonic tests for ALS prognoses; clinical diagnoses of the disease take time and are usually difficult. Prognostic biomarkers are urgently needed for rapid and effective ALS prognoses. Male albino rats were divided into ten groups based on age: 0 (40–45 days old), A (70–75 days old), B (90–95 days old), C (110–115 days old), and D (130–135 days old). Each group was divided into two subgroups according to its mutation status: wild type (SOD1<sup>WT</sup>) or mutated (SOD1<sup>G93A</sup>). Serum biochemistry and hematological parameters were measured in 90 rats to evaluate possible biomarkers for faster ALS diagnoses and prognoses. Weight loss, cholesterol, creatinine, glucose, total bilirubin (TBIL), blood urine nitrogen (BUN), c-peptide, glucagon, PYY, white blood cell (WBC), lymphocyte (LYM), monocyte (MID), granulocyte (GRAN), red cell distribution width with standard deviation (RDW-SD), red cell distribution width with the coefficient of variation (RDW-CV), platelet (PLT), mean platelet volume (MPV), platelet distribution width (PDW), and procalcitonin (PCT) levels were changed in the SOD1<sup>G93A</sup> rats compared to the SOD1<sup>WT</sup> rats independently from aging. For the first time in the literature, we showed promising hematological and serum biochemistry parameters in the pre-symptomatic and symptomatic stages of ALS by eliminating the effects of aging. Our results can be used for early diagnoses and prognoses of ALS, improving the quality of life and survival time of ALS patients. |
| first_indexed | 2024-03-09T18:25:25Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-09T18:25:25Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Cells |
| spelling | doaj.art-6d68ca7940a0467188abe1fdb15956e42023-11-24T07:57:33ZengMDPI AGCells2073-44092022-11-011122356910.3390/cells11223569Evaluation of the Hematological and Serum Biochemistry Parameters in the Pre-Symptomatic and Symptomatic Stages of ALS Disease to Support Early Diagnosis and PrognosisDuygu Aydemir0Selcuk Surucu1Ayse Nazli Basak2Nuriye Nuray Ulusu3Department of Medical Biochemistry, School of Medicine, Koc University, Sariyer, Istanbul 34450, TurkeyDepartment of Anatomy, School of Medicine, Koc University, Sariyer, Istanbul 34450, TurkeySuna and İnan Kıraç Foundation, Neurodegeneration Research Laboratory, NDAL-KUTTAM, School of Medicine, Koç University, Istanbul 34010, TurkeyDepartment of Medical Biochemistry, School of Medicine, Koc University, Sariyer, Istanbul 34450, TurkeyAmyotrophic lateral sclerosis (ALS) is the most common motor neuron disease. Since there are no pathognomonic tests for ALS prognoses; clinical diagnoses of the disease take time and are usually difficult. Prognostic biomarkers are urgently needed for rapid and effective ALS prognoses. Male albino rats were divided into ten groups based on age: 0 (40–45 days old), A (70–75 days old), B (90–95 days old), C (110–115 days old), and D (130–135 days old). Each group was divided into two subgroups according to its mutation status: wild type (SOD1<sup>WT</sup>) or mutated (SOD1<sup>G93A</sup>). Serum biochemistry and hematological parameters were measured in 90 rats to evaluate possible biomarkers for faster ALS diagnoses and prognoses. Weight loss, cholesterol, creatinine, glucose, total bilirubin (TBIL), blood urine nitrogen (BUN), c-peptide, glucagon, PYY, white blood cell (WBC), lymphocyte (LYM), monocyte (MID), granulocyte (GRAN), red cell distribution width with standard deviation (RDW-SD), red cell distribution width with the coefficient of variation (RDW-CV), platelet (PLT), mean platelet volume (MPV), platelet distribution width (PDW), and procalcitonin (PCT) levels were changed in the SOD1<sup>G93A</sup> rats compared to the SOD1<sup>WT</sup> rats independently from aging. For the first time in the literature, we showed promising hematological and serum biochemistry parameters in the pre-symptomatic and symptomatic stages of ALS by eliminating the effects of aging. Our results can be used for early diagnoses and prognoses of ALS, improving the quality of life and survival time of ALS patients.https://www.mdpi.com/2073-4409/11/22/3569ALSserum biochemistryhematological parametersearly diagnosispre-symptomaticsymptomatic |
| spellingShingle | Duygu Aydemir Selcuk Surucu Ayse Nazli Basak Nuriye Nuray Ulusu Evaluation of the Hematological and Serum Biochemistry Parameters in the Pre-Symptomatic and Symptomatic Stages of ALS Disease to Support Early Diagnosis and Prognosis Cells ALS serum biochemistry hematological parameters early diagnosis pre-symptomatic symptomatic |
| title | Evaluation of the Hematological and Serum Biochemistry Parameters in the Pre-Symptomatic and Symptomatic Stages of ALS Disease to Support Early Diagnosis and Prognosis |
| title_full | Evaluation of the Hematological and Serum Biochemistry Parameters in the Pre-Symptomatic and Symptomatic Stages of ALS Disease to Support Early Diagnosis and Prognosis |
| title_fullStr | Evaluation of the Hematological and Serum Biochemistry Parameters in the Pre-Symptomatic and Symptomatic Stages of ALS Disease to Support Early Diagnosis and Prognosis |
| title_full_unstemmed | Evaluation of the Hematological and Serum Biochemistry Parameters in the Pre-Symptomatic and Symptomatic Stages of ALS Disease to Support Early Diagnosis and Prognosis |
| title_short | Evaluation of the Hematological and Serum Biochemistry Parameters in the Pre-Symptomatic and Symptomatic Stages of ALS Disease to Support Early Diagnosis and Prognosis |
| title_sort | evaluation of the hematological and serum biochemistry parameters in the pre symptomatic and symptomatic stages of als disease to support early diagnosis and prognosis |
| topic | ALS serum biochemistry hematological parameters early diagnosis pre-symptomatic symptomatic |
| url | https://www.mdpi.com/2073-4409/11/22/3569 |
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