Combination effects of DNase I and tobramycin on survival and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae isolated from chronic rhinosinusitis patients
This study aimed to investigate the effects of DNase I in combination with tobramycin (TOB) on drug susceptibility and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae. Biofilm formation, eDNA production and drug susceptibilities of P. aeruginosa and K. pneumoniae in both...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2022-10-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://sjst.psu.ac.th/journal/44-5/20.pdf |
| _version_ | 1797839731628703744 |
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| author | Chitchanok Anutrakunchai Saharut Wongkaewkhiaw Sakawrat Kanthawong Sorujsiri Chareonsudjai Rattiyaphorn Pakkulnan Suwimol Taweechaisupapong |
| author_facet | Chitchanok Anutrakunchai Saharut Wongkaewkhiaw Sakawrat Kanthawong Sorujsiri Chareonsudjai Rattiyaphorn Pakkulnan Suwimol Taweechaisupapong |
| author_sort | Chitchanok Anutrakunchai |
| collection | DOAJ |
| description | This study aimed to investigate the effects of DNase I in combination with tobramycin (TOB) on drug susceptibility
and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae. Biofilm formation, eDNA production and
drug susceptibilities of P. aeruginosa and K. pneumoniae in both planktonic and biofilm forms were evaluated under different
nutrient concentrations. The biofilm architectures of both bacteria were evaluated using confocal laser scanning microscopy. The
results showed that the biofilm formations and eDNA quantities of both bacteria were reduced in a nutrient-limited condition.
The eDNA concentrations in the biofilms were correlated with the amount of biofilm formed. Moreover, a combination with
DNase I can enhance the bactericidal activity of TOB and decrease biofilm formation of both bacteria compared with TOB alone.
These results suggest that the combined effects of DNase I and TOB allows prevention of biofilm formation and subsequent
penetration of the drug resulting in an increased anti-biofilm effect. |
| first_indexed | 2024-04-09T16:02:53Z |
| format | Article |
| id | doaj.art-6d6d45d2736f4940a5ffffe40d16cab1 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-09T16:02:53Z |
| publishDate | 2022-10-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-6d6d45d2736f4940a5ffffe40d16cab12023-04-25T09:32:39ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952022-10-014451306131310.14456/sjst-psu.2022.170Combination effects of DNase I and tobramycin on survival and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae isolated from chronic rhinosinusitis patientsChitchanok Anutrakunchai0Saharut Wongkaewkhiaw1Sakawrat Kanthawong2Sorujsiri Chareonsudjai3Rattiyaphorn Pakkulnan4Suwimol Taweechaisupapong5Biofilm Research Group, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandBiofilm Research Group, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandBiofilm Research Group, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandBiofilm Research Group, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandBiofilm Research Group, Faculty of Dentistry, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandThis study aimed to investigate the effects of DNase I in combination with tobramycin (TOB) on drug susceptibility and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae. Biofilm formation, eDNA production and drug susceptibilities of P. aeruginosa and K. pneumoniae in both planktonic and biofilm forms were evaluated under different nutrient concentrations. The biofilm architectures of both bacteria were evaluated using confocal laser scanning microscopy. The results showed that the biofilm formations and eDNA quantities of both bacteria were reduced in a nutrient-limited condition. The eDNA concentrations in the biofilms were correlated with the amount of biofilm formed. Moreover, a combination with DNase I can enhance the bactericidal activity of TOB and decrease biofilm formation of both bacteria compared with TOB alone. These results suggest that the combined effects of DNase I and TOB allows prevention of biofilm formation and subsequent penetration of the drug resulting in an increased anti-biofilm effect.https://sjst.psu.ac.th/journal/44-5/20.pdfdnase iednaklebsiella pneumoniapseudomonas aeruginosatobramycin |
| spellingShingle | Chitchanok Anutrakunchai Saharut Wongkaewkhiaw Sakawrat Kanthawong Sorujsiri Chareonsudjai Rattiyaphorn Pakkulnan Suwimol Taweechaisupapong Combination effects of DNase I and tobramycin on survival and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae isolated from chronic rhinosinusitis patients Songklanakarin Journal of Science and Technology (SJST) dnase i edna klebsiella pneumonia pseudomonas aeruginosa tobramycin |
| title | Combination effects of DNase I and tobramycin on survival and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae isolated from chronic rhinosinusitis patients |
| title_full | Combination effects of DNase I and tobramycin on survival and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae isolated from chronic rhinosinusitis patients |
| title_fullStr | Combination effects of DNase I and tobramycin on survival and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae isolated from chronic rhinosinusitis patients |
| title_full_unstemmed | Combination effects of DNase I and tobramycin on survival and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae isolated from chronic rhinosinusitis patients |
| title_short | Combination effects of DNase I and tobramycin on survival and biofilm architectures of Pseudomonas aeruginosa and Klebsiella pneumoniae isolated from chronic rhinosinusitis patients |
| title_sort | combination effects of dnase i and tobramycin on survival and biofilm architectures of pseudomonas aeruginosa and klebsiella pneumoniae isolated from chronic rhinosinusitis patients |
| topic | dnase i edna klebsiella pneumonia pseudomonas aeruginosa tobramycin |
| url | https://sjst.psu.ac.th/journal/44-5/20.pdf |
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