SIMULTANEOUS IDENTIFICATION, QUANTIFICATION, AND ANALYSIS OF MAIN COMPONENTS OF “HAIRY” ROOT EXTRACTS OF Artemisia annua AND Artemisia tilesii PLANTS
Aim. The profiles of polyphenolic phytochemicals in extracts of “hairy” roots of Artemisia tilesii Ledeb. and Artemisia annua L. were studied. Analytical separation and quantification of main components in extracts were evaluated. Methods. “hairy” roots were grown in vitro on Murashige and Skoog...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2021-12-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/stories/6_2021/kobylinska_6_2021.pdf |
| _version_ | 1797757967566635008 |
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| author | N. Kobylinska T. Bohdanovych V. Duplij Pashchenko N. Matvieieva |
| author_facet | N. Kobylinska T. Bohdanovych V. Duplij Pashchenko N. Matvieieva |
| author_sort | N. Kobylinska |
| collection | DOAJ |
| description | Aim. The profiles of polyphenolic phytochemicals in extracts of “hairy” roots of Artemisia tilesii Ledeb. and Artemisia annua L. were studied. Analytical separation and quantification of main components in extracts were evaluated.
Methods. “hairy” roots were grown in vitro on Murashige and Skoog medium. High-performance chromatography coupled with different types of detection (photo diode array detection (DAD) and electrospray ionization with ultra-high resolution Qq-Time-of-Flight mass spectrometry) was used to identify and quantify the main biologically active components in ethanol extracts of “hairy” roots.
Results. The amount of flavonoids was 94.71–144.33 mg RE/g DW and 33.52–78.00 mg RE/g DW in “hairy” roots of A. annua and A. tilesii, respectively. In most samples of “hairy” roots, the amount of flavonoids was higher than the content in the control plant roots. The presence of Apigenin (0.168 ± 0.003 mg/L and 0.178 ± 0.006 mg/L), Quercetin (0.282 ± 0.005 mg/L and 0.174 ± 0.005 mg/L) in the extracts of A. annua and A. tilesii was shown by reverse-phase HPLC-DAD method. Chlorogenic acid, Kaempferol, and other flavonoids were detected.
Conclusions. The developed HPLC-DAD method demonstrated the high percentage of recovery, low limit of detection and quantification (9,11 ng/ml ≤ LOQ ≤16,51 ng/ml), accuracy and correctness. Thus, the method is suitable for the simultaneous quantification of phenolic acids and flavonoids in various plant extracts with short time and high efficiency. |
| first_indexed | 2024-03-12T18:23:07Z |
| format | Article |
| id | doaj.art-6d6d641b70c741948a55bde5c5008c5f |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T18:23:07Z |
| publishDate | 2021-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-6d6d641b70c741948a55bde5c5008c5f2023-08-02T08:41:20ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2021-12-01146607010.15407/biotech14.06.060SIMULTANEOUS IDENTIFICATION, QUANTIFICATION, AND ANALYSIS OF MAIN COMPONENTS OF “HAIRY” ROOT EXTRACTS OF Artemisia annua AND Artemisia tilesii PLANTSN. Kobylinska0T. Bohdanovych1V. Duplij 2Pashchenko 3N. Matvieieva4Dumansky Institute of Colloid and Water Chemistry of of the National Academy of Sciences of Ukraine, KyivInstitute of Cell Biology and Genetic Engineering of of the National Academy of Sciences of Ukraine, KyivInstitute of Cell Biology and Genetic Engineering of of the National Academy of Sciences of Ukraine, Kyiv Kyiv National University of Technologies and Design, UkraineDumansky Institute of Colloid and Water Chemistry of of the National Academy of Sciences of Ukraine, KyivAim. The profiles of polyphenolic phytochemicals in extracts of “hairy” roots of Artemisia tilesii Ledeb. and Artemisia annua L. were studied. Analytical separation and quantification of main components in extracts were evaluated. Methods. “hairy” roots were grown in vitro on Murashige and Skoog medium. High-performance chromatography coupled with different types of detection (photo diode array detection (DAD) and electrospray ionization with ultra-high resolution Qq-Time-of-Flight mass spectrometry) was used to identify and quantify the main biologically active components in ethanol extracts of “hairy” roots. Results. The amount of flavonoids was 94.71–144.33 mg RE/g DW and 33.52–78.00 mg RE/g DW in “hairy” roots of A. annua and A. tilesii, respectively. In most samples of “hairy” roots, the amount of flavonoids was higher than the content in the control plant roots. The presence of Apigenin (0.168 ± 0.003 mg/L and 0.178 ± 0.006 mg/L), Quercetin (0.282 ± 0.005 mg/L and 0.174 ± 0.005 mg/L) in the extracts of A. annua and A. tilesii was shown by reverse-phase HPLC-DAD method. Chlorogenic acid, Kaempferol, and other flavonoids were detected. Conclusions. The developed HPLC-DAD method demonstrated the high percentage of recovery, low limit of detection and quantification (9,11 ng/ml ≤ LOQ ≤16,51 ng/ml), accuracy and correctness. Thus, the method is suitable for the simultaneous quantification of phenolic acids and flavonoids in various plant extracts with short time and high efficiency.http://biotechnology.kiev.ua/images/stories/6_2021/kobylinska_6_2021.pdfartemisia tilesiiartemisia annuapolyphenolsflavonoids“hairy” rootsreversed-phase hplc with diode matrix detector. |
| spellingShingle | N. Kobylinska T. Bohdanovych V. Duplij Pashchenko N. Matvieieva SIMULTANEOUS IDENTIFICATION, QUANTIFICATION, AND ANALYSIS OF MAIN COMPONENTS OF “HAIRY” ROOT EXTRACTS OF Artemisia annua AND Artemisia tilesii PLANTS Biotechnologia Acta artemisia tilesii artemisia annua polyphenols flavonoids “hairy” roots reversed-phase hplc with diode matrix detector. |
| title | SIMULTANEOUS IDENTIFICATION, QUANTIFICATION, AND ANALYSIS OF MAIN COMPONENTS OF “HAIRY” ROOT EXTRACTS OF Artemisia annua AND Artemisia tilesii PLANTS |
| title_full | SIMULTANEOUS IDENTIFICATION, QUANTIFICATION, AND ANALYSIS OF MAIN COMPONENTS OF “HAIRY” ROOT EXTRACTS OF Artemisia annua AND Artemisia tilesii PLANTS |
| title_fullStr | SIMULTANEOUS IDENTIFICATION, QUANTIFICATION, AND ANALYSIS OF MAIN COMPONENTS OF “HAIRY” ROOT EXTRACTS OF Artemisia annua AND Artemisia tilesii PLANTS |
| title_full_unstemmed | SIMULTANEOUS IDENTIFICATION, QUANTIFICATION, AND ANALYSIS OF MAIN COMPONENTS OF “HAIRY” ROOT EXTRACTS OF Artemisia annua AND Artemisia tilesii PLANTS |
| title_short | SIMULTANEOUS IDENTIFICATION, QUANTIFICATION, AND ANALYSIS OF MAIN COMPONENTS OF “HAIRY” ROOT EXTRACTS OF Artemisia annua AND Artemisia tilesii PLANTS |
| title_sort | simultaneous identification quantification and analysis of main components of hairy root extracts of artemisia annua and artemisia tilesii plants |
| topic | artemisia tilesii artemisia annua polyphenols flavonoids “hairy” roots reversed-phase hplc with diode matrix detector. |
| url | http://biotechnology.kiev.ua/images/stories/6_2021/kobylinska_6_2021.pdf |
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