DETERMINAÇÃO ESPECTROFOTOMÉTRICA DE METILDOPA EM ENSAIO DE DISSOLUÇÃO DE COMPRIMIDOS UTILIZANDO EXTRATO DE RABANETE COMO FONTE DE PEROXIDASE
An enzymatic spectrophotometric method for the determination of methyldopa in a dissolution test of tablets was developed using peroxidase from radish (Raphanus sativus). The enzyme was extracted from radish roots using a phosphate buffer of pH 6.5 and partially purified through centrifugation. The...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2015-09-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000801107&lng=en&tlng=en |
| _version_ | 1818203734510927872 |
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| author | Natielle Gianine Bueno Airton Vicente Pereira |
| author_facet | Natielle Gianine Bueno Airton Vicente Pereira |
| author_sort | Natielle Gianine Bueno |
| collection | DOAJ |
| description | An enzymatic spectrophotometric method for the determination of methyldopa in a dissolution test of tablets was developed using peroxidase from radish (Raphanus sativus). The enzyme was extracted from radish roots using a phosphate buffer of pH 6.5 and partially purified through centrifugation. The supernatant was used as a source of peroxidase. The methyldopachrome resulting from the oxidation of methyldopa catalyzed by peroxidase was monitored at 480 nm. The enzymatic activity was stable for a period of at least 25 days when the extract was stored at 4 or -20 ºC. The method was validated according to RDC 899 and ICH guidelines. The calibration graph was linear in the range 200-800 µg mL-1, with a correlation coefficient of 0.9992. The limits of detection and quantification in the dissolution medium were 36 and 120 µg mL-1, respectively. Recovery was greater than 98.9%. This method can be applied for the determination of methyldopa in dissolution tests of tablets without interference from the excipients. |
| first_indexed | 2024-12-12T03:30:03Z |
| format | Article |
| id | doaj.art-6d6f276e6c53475fb64a1b2d324fc1ca |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-12-12T03:30:03Z |
| publishDate | 2015-09-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-6d6f276e6c53475fb64a1b2d324fc1ca2022-12-22T00:39:56ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642015-09-013881107111110.5935/0100-4042.20150091S0100-40422015000801107DETERMINAÇÃO ESPECTROFOTOMÉTRICA DE METILDOPA EM ENSAIO DE DISSOLUÇÃO DE COMPRIMIDOS UTILIZANDO EXTRATO DE RABANETE COMO FONTE DE PEROXIDASENatielle Gianine BuenoAirton Vicente PereiraAn enzymatic spectrophotometric method for the determination of methyldopa in a dissolution test of tablets was developed using peroxidase from radish (Raphanus sativus). The enzyme was extracted from radish roots using a phosphate buffer of pH 6.5 and partially purified through centrifugation. The supernatant was used as a source of peroxidase. The methyldopachrome resulting from the oxidation of methyldopa catalyzed by peroxidase was monitored at 480 nm. The enzymatic activity was stable for a period of at least 25 days when the extract was stored at 4 or -20 ºC. The method was validated according to RDC 899 and ICH guidelines. The calibration graph was linear in the range 200-800 µg mL-1, with a correlation coefficient of 0.9992. The limits of detection and quantification in the dissolution medium were 36 and 120 µg mL-1, respectively. Recovery was greater than 98.9%. This method can be applied for the determination of methyldopa in dissolution tests of tablets without interference from the excipients.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000801107&lng=en&tlng=enmethyldopadissolution testradish rootsperoxidase |
| spellingShingle | Natielle Gianine Bueno Airton Vicente Pereira DETERMINAÇÃO ESPECTROFOTOMÉTRICA DE METILDOPA EM ENSAIO DE DISSOLUÇÃO DE COMPRIMIDOS UTILIZANDO EXTRATO DE RABANETE COMO FONTE DE PEROXIDASE Química Nova methyldopa dissolution test radish roots peroxidase |
| title | DETERMINAÇÃO ESPECTROFOTOMÉTRICA DE METILDOPA EM ENSAIO DE DISSOLUÇÃO DE COMPRIMIDOS UTILIZANDO EXTRATO DE RABANETE COMO FONTE DE PEROXIDASE |
| title_full | DETERMINAÇÃO ESPECTROFOTOMÉTRICA DE METILDOPA EM ENSAIO DE DISSOLUÇÃO DE COMPRIMIDOS UTILIZANDO EXTRATO DE RABANETE COMO FONTE DE PEROXIDASE |
| title_fullStr | DETERMINAÇÃO ESPECTROFOTOMÉTRICA DE METILDOPA EM ENSAIO DE DISSOLUÇÃO DE COMPRIMIDOS UTILIZANDO EXTRATO DE RABANETE COMO FONTE DE PEROXIDASE |
| title_full_unstemmed | DETERMINAÇÃO ESPECTROFOTOMÉTRICA DE METILDOPA EM ENSAIO DE DISSOLUÇÃO DE COMPRIMIDOS UTILIZANDO EXTRATO DE RABANETE COMO FONTE DE PEROXIDASE |
| title_short | DETERMINAÇÃO ESPECTROFOTOMÉTRICA DE METILDOPA EM ENSAIO DE DISSOLUÇÃO DE COMPRIMIDOS UTILIZANDO EXTRATO DE RABANETE COMO FONTE DE PEROXIDASE |
| title_sort | determinacao espectrofotometrica de metildopa em ensaio de dissolucao de comprimidos utilizando extrato de rabanete como fonte de peroxidase |
| topic | methyldopa dissolution test radish roots peroxidase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000801107&lng=en&tlng=en |
| work_keys_str_mv | AT natiellegianinebueno determinacaoespectrofotometricademetildopaemensaiodedissolucaodecomprimidosutilizandoextratoderabanetecomofontedeperoxidase AT airtonvicentepereira determinacaoespectrofotometricademetildopaemensaiodedissolucaodecomprimidosutilizandoextratoderabanetecomofontedeperoxidase |