RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue
During the early postnatal period, lambs have the ability to thermoregulate body temperature via non-shivering thermogenesis through brown adipose tissue (BAT), which soon after birth begins to transform into white adipose tissue. An RNA seq approach was used to characterize the transcriptome of BAT...
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MDPI AG
2022-10-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/10/996 |
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| author | Andrea Graña-Baumgartner Venkata S. R. Dukkipati Paul R. Kenyon Hugh T. Blair Nicolás López-Villalobos Kristene Gedye Patrick J. Biggs |
| author_facet | Andrea Graña-Baumgartner Venkata S. R. Dukkipati Paul R. Kenyon Hugh T. Blair Nicolás López-Villalobos Kristene Gedye Patrick J. Biggs |
| author_sort | Andrea Graña-Baumgartner |
| collection | DOAJ |
| description | During the early postnatal period, lambs have the ability to thermoregulate body temperature via non-shivering thermogenesis through brown adipose tissue (BAT), which soon after birth begins to transform into white adipose tissue. An RNA seq approach was used to characterize the transcriptome of BAT and thyroid tissue in newborn lambs exposed to cold conditions. Fifteen newborn Romney lambs were selected and divided into three groups: group 1 (<i>n</i> = 3) was a control, and groups 2 and 3 (<i>n</i> = 6 each) were kept indoors for two days at an ambient temperature (20–22 °C) or at a cold temperature (4 °C), respectively. Sequencing was performed using a paired-end strategy through the BGISEQ-500 platform, followed by the identification of differentially expressed genes using DESeq2 and an enrichment analysis by g:Profiler. This study provides an in-depth expression network of the main characters involved in the thermogenesis and fat-whitening mechanisms that take place in the newborn lamb. Data revealed no significant differential expression of key thermogenic factors such as uncoupling protein 1, suggesting that the heat production peak under cold exposure might occur so rapidly and in such an immediate way that it may seem undetectable in BAT by day three of life. Moreover, these changes in expression might indicate the start of the whitening process of the adipose tissue, concluding the non-shivering thermogenesis period. |
| first_indexed | 2024-03-09T19:49:14Z |
| format | Article |
| id | doaj.art-6d73f447485a4a2fba94f1b8d5e8f264 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T19:49:14Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-6d73f447485a4a2fba94f1b8d5e8f2642023-11-24T01:16:35ZengMDPI AGMetabolites2218-19892022-10-01121099610.3390/metabo12100996RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose TissueAndrea Graña-Baumgartner0Venkata S. R. Dukkipati1Paul R. Kenyon2Hugh T. Blair3Nicolás López-Villalobos4Kristene Gedye5Patrick J. Biggs6School of Agriculture and Environment, Massey University, Private Bag 11 222, Palmerston North 4442, New ZealandSchool of Agriculture and Environment, Massey University, Private Bag 11 222, Palmerston North 4442, New ZealandSchool of Agriculture and Environment, Massey University, Private Bag 11 222, Palmerston North 4442, New ZealandSchool of Agriculture and Environment, Massey University, Private Bag 11 222, Palmerston North 4442, New ZealandSchool of Agriculture and Environment, Massey University, Private Bag 11 222, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Private Bag 11 222, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Private Bag 11 222, Palmerston North 4442, New ZealandDuring the early postnatal period, lambs have the ability to thermoregulate body temperature via non-shivering thermogenesis through brown adipose tissue (BAT), which soon after birth begins to transform into white adipose tissue. An RNA seq approach was used to characterize the transcriptome of BAT and thyroid tissue in newborn lambs exposed to cold conditions. Fifteen newborn Romney lambs were selected and divided into three groups: group 1 (<i>n</i> = 3) was a control, and groups 2 and 3 (<i>n</i> = 6 each) were kept indoors for two days at an ambient temperature (20–22 °C) or at a cold temperature (4 °C), respectively. Sequencing was performed using a paired-end strategy through the BGISEQ-500 platform, followed by the identification of differentially expressed genes using DESeq2 and an enrichment analysis by g:Profiler. This study provides an in-depth expression network of the main characters involved in the thermogenesis and fat-whitening mechanisms that take place in the newborn lamb. Data revealed no significant differential expression of key thermogenic factors such as uncoupling protein 1, suggesting that the heat production peak under cold exposure might occur so rapidly and in such an immediate way that it may seem undetectable in BAT by day three of life. Moreover, these changes in expression might indicate the start of the whitening process of the adipose tissue, concluding the non-shivering thermogenesis period.https://www.mdpi.com/2218-1989/12/10/996RNAseqBATthermogenesisfat whiteninglambs |
| spellingShingle | Andrea Graña-Baumgartner Venkata S. R. Dukkipati Paul R. Kenyon Hugh T. Blair Nicolás López-Villalobos Kristene Gedye Patrick J. Biggs RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue Metabolites RNAseq BAT thermogenesis fat whitening lambs |
| title | RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue |
| title_full | RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue |
| title_fullStr | RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue |
| title_full_unstemmed | RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue |
| title_short | RNAseq Analysis of Brown Adipose Tissue and Thyroid of Newborn Lambs Subjected to Short-Term Cold Exposure Reveals Signs of Early Whitening of Adipose Tissue |
| title_sort | rnaseq analysis of brown adipose tissue and thyroid of newborn lambs subjected to short term cold exposure reveals signs of early whitening of adipose tissue |
| topic | RNAseq BAT thermogenesis fat whitening lambs |
| url | https://www.mdpi.com/2218-1989/12/10/996 |
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