Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila
Summary: RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolut...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723007349 |
| _version_ | 1797791879941586944 |
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| author | Harrison A. Curnutte Xinyue Lan Manuel Sargen Si Man Ao Ieong Dylan Campbell Hyosik Kim Yijun Liao Sarah Bailah Lazar Tatjana Trcek |
| author_facet | Harrison A. Curnutte Xinyue Lan Manuel Sargen Si Man Ao Ieong Dylan Campbell Hyosik Kim Yijun Liao Sarah Bailah Lazar Tatjana Trcek |
| author_sort | Harrison A. Curnutte |
| collection | DOAJ |
| description | Summary: RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolution microscopy reveals that the number, size, and distribution of germ granules is precisely controlled. Surprisingly, germ granule mRNAs are not required for the nucleation or the persistence of germ granules but instead control their size and composition. Using an RNAi screen, we determine that RNA regulators, helicases, and mitochondrial proteins regulate germ granule number and size, while the proteins of the endoplasmic reticulum, nuclear pore complex, and cytoskeleton control their distribution. Therefore, the protein-driven formation of Drosophila germ granules is mechanistically distinct from the RNA-dependent condensation observed for other RNA granules such as stress granules and P-bodies. |
| first_indexed | 2024-03-13T02:25:08Z |
| format | Article |
| id | doaj.art-6d75b331520e48e0be9f822f751d38c8 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-13T02:25:08Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-6d75b331520e48e0be9f822f751d38c82023-06-30T04:21:54ZengElsevierCell Reports2211-12472023-07-01427112723Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in DrosophilaHarrison A. Curnutte0Xinyue Lan1Manuel Sargen2Si Man Ao Ieong3Dylan Campbell4Hyosik Kim5Yijun Liao6Sarah Bailah Lazar7Tatjana Trcek8Department of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USADepartment of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA; Corresponding authorSummary: RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolution microscopy reveals that the number, size, and distribution of germ granules is precisely controlled. Surprisingly, germ granule mRNAs are not required for the nucleation or the persistence of germ granules but instead control their size and composition. Using an RNAi screen, we determine that RNA regulators, helicases, and mitochondrial proteins regulate germ granule number and size, while the proteins of the endoplasmic reticulum, nuclear pore complex, and cytoskeleton control their distribution. Therefore, the protein-driven formation of Drosophila germ granules is mechanistically distinct from the RNA-dependent condensation observed for other RNA granules such as stress granules and P-bodies.http://www.sciencedirect.com/science/article/pii/S2211124723007349CP: Cell biology |
| spellingShingle | Harrison A. Curnutte Xinyue Lan Manuel Sargen Si Man Ao Ieong Dylan Campbell Hyosik Kim Yijun Liao Sarah Bailah Lazar Tatjana Trcek Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila Cell Reports CP: Cell biology |
| title | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
| title_full | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
| title_fullStr | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
| title_full_unstemmed | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
| title_short | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
| title_sort | proteins rather than mrnas regulate nucleation and persistence of oskar germ granules in drosophila |
| topic | CP: Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723007349 |
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