The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosis
Abstract Objective Endometriosis is a common and painful condition characterised by the formation of endometrial lesions within the peritoneal cavity. Previous studies have suggested a role for hedgehog signalling in the pathogenesis of endometriosis. We investigated the role of hedgehog signalling...
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | BMC Research Notes |
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| Online Access: | http://link.springer.com/article/10.1186/s13104-020-05299-5 |
| _version_ | 1819051495596752896 |
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| author | F. L. Cousins J. K. Farley R. Kerrigan S. Mukherjee S. Darzi C. E. Gargett J. A. Deane |
| author_facet | F. L. Cousins J. K. Farley R. Kerrigan S. Mukherjee S. Darzi C. E. Gargett J. A. Deane |
| author_sort | F. L. Cousins |
| collection | DOAJ |
| description | Abstract Objective Endometriosis is a common and painful condition characterised by the formation of endometrial lesions within the peritoneal cavity. Previous studies have suggested a role for hedgehog signalling in the pathogenesis of endometriosis. We investigated the role of hedgehog signalling in the establishment of endometriosis lesions using 5E1, a hedgehog ligand neutralising antibody, and a mouse model of endometriosis. To mimic the initiation of endometriosis by retrograde menstruation, which is believed to occur in humans, donor mice underwent an artificial menstruation protocol. Fragments of menstrual endometrium were injected into the peritoneal cavity of estrogen primed recipients. Recipients received twice weekly injections of 5E1 or an isotype matched control antibody for three weeks. Lesions were collected and analysed for markers of epithelium, proliferation and apoptosis by immunofluorescence microscopy. Results Treatment with 5E1 reduced the number of lesions found on the mesentery. No significant changes were found in the size of lesions, abundance of endometrial epithelial cells, proliferation or apoptosis. |
| first_indexed | 2024-12-21T12:04:51Z |
| format | Article |
| id | doaj.art-6d77f200d5724218a8853b91691a1ec2 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-21T12:04:51Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-6d77f200d5724218a8853b91691a1ec22022-12-21T19:04:43ZengBMCBMC Research Notes1756-05002020-09-011311610.1186/s13104-020-05299-5The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosisF. L. Cousins0J. K. Farley1R. Kerrigan2S. Mukherjee3S. Darzi4C. E. Gargett5J. A. Deane6The Ritchie Centre, Hudson Institute of Medical ResearchThe Ritchie Centre, Hudson Institute of Medical ResearchThe Ritchie Centre, Hudson Institute of Medical ResearchThe Ritchie Centre, Hudson Institute of Medical ResearchThe Ritchie Centre, Hudson Institute of Medical ResearchThe Ritchie Centre, Hudson Institute of Medical ResearchDepartment of Obstetrics and Gynaecology, School of Clinical Sciences At Monash Health, Monash University Faculty of MedicineAbstract Objective Endometriosis is a common and painful condition characterised by the formation of endometrial lesions within the peritoneal cavity. Previous studies have suggested a role for hedgehog signalling in the pathogenesis of endometriosis. We investigated the role of hedgehog signalling in the establishment of endometriosis lesions using 5E1, a hedgehog ligand neutralising antibody, and a mouse model of endometriosis. To mimic the initiation of endometriosis by retrograde menstruation, which is believed to occur in humans, donor mice underwent an artificial menstruation protocol. Fragments of menstrual endometrium were injected into the peritoneal cavity of estrogen primed recipients. Recipients received twice weekly injections of 5E1 or an isotype matched control antibody for three weeks. Lesions were collected and analysed for markers of epithelium, proliferation and apoptosis by immunofluorescence microscopy. Results Treatment with 5E1 reduced the number of lesions found on the mesentery. No significant changes were found in the size of lesions, abundance of endometrial epithelial cells, proliferation or apoptosis.http://link.springer.com/article/10.1186/s13104-020-05299-5EndometriumEndometriosisHedgehog signallingMouse model |
| spellingShingle | F. L. Cousins J. K. Farley R. Kerrigan S. Mukherjee S. Darzi C. E. Gargett J. A. Deane The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosis BMC Research Notes Endometrium Endometriosis Hedgehog signalling Mouse model |
| title | The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosis |
| title_full | The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosis |
| title_fullStr | The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosis |
| title_full_unstemmed | The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosis |
| title_short | The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosis |
| title_sort | effects of hedgehog ligand neutralising antibody 5e1 in a mouse model of endometriosis |
| topic | Endometrium Endometriosis Hedgehog signalling Mouse model |
| url | http://link.springer.com/article/10.1186/s13104-020-05299-5 |
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