Isolation and Purification of Chitosan Oligosaccharides (Mw ≤ 1000) and Their Protective Effect on Acute Liver Injury Caused by CCl<sub>4</sub>
Chitosan oligosaccharides are the degradation products of chitin obtained from the shell extracts of shrimps and crabs. Compared with chitosan, chitosan oligosaccharides have better solubility and a wider application range. In this study, high-molecular-weight chitosan oligosaccharides (COST, chitos...
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MDPI AG
2024-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/22/3/128 |
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| author | Kai Wang Dawei Yu Yan Bai Hua Cao Jiao Guo Zhengquan Su |
| author_facet | Kai Wang Dawei Yu Yan Bai Hua Cao Jiao Guo Zhengquan Su |
| author_sort | Kai Wang |
| collection | DOAJ |
| description | Chitosan oligosaccharides are the degradation products of chitin obtained from the shell extracts of shrimps and crabs. Compared with chitosan, chitosan oligosaccharides have better solubility and a wider application range. In this study, high-molecular-weight chitosan oligosaccharides (COST, chitosan oligosaccharides, MW ≤ 1000) were isolated and purified by a GPC gel column, and the molecular weight range was further reduced to obtain high-purity and low-molecular-weight chitosan (COS46). Compared with COST, COS46 is better at inhibiting CCl<sub>4</sub>-induced cell death, improving cell morphology, reducing ALT content, and improving cell antioxidant capacity. The effects of COST and COS46 on CCl<sub>4</sub>-induced acute liver injury were further verified in mice. Both COS46 and COST improved the appearance of the liver induced by CCl<sub>4</sub>, decreased the levels of ALT and AST in serum, and decreased the oxidation/antioxidant index in the liver. From the liver pathological section, the effect of COS46 was better. In addition, some indicators of COS46 showed a dose-dependent effect. In conclusion, compared with COST, low-molecular-weight COS46 has better antioxidant capacity and a better therapeutic effect on CCl<sub>4</sub>-induced acute liver injury. |
| first_indexed | 2024-04-24T18:03:53Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-24T18:03:53Z |
| publishDate | 2024-03-01 |
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| series | Marine Drugs |
| spelling | doaj.art-6d78314098884c9db7703821b023dec72024-03-27T13:52:13ZengMDPI AGMarine Drugs1660-33972024-03-0122312810.3390/md22030128Isolation and Purification of Chitosan Oligosaccharides (Mw ≤ 1000) and Their Protective Effect on Acute Liver Injury Caused by CCl<sub>4</sub>Kai Wang0Dawei Yu1Yan Bai2Hua Cao3Jiao Guo4Zhengquan Su5Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaGuangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, ChinaSchool of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458, ChinaGuangdong Metabolic Disease Research Center of Integrated Chinese and Western Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaGuangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaChitosan oligosaccharides are the degradation products of chitin obtained from the shell extracts of shrimps and crabs. Compared with chitosan, chitosan oligosaccharides have better solubility and a wider application range. In this study, high-molecular-weight chitosan oligosaccharides (COST, chitosan oligosaccharides, MW ≤ 1000) were isolated and purified by a GPC gel column, and the molecular weight range was further reduced to obtain high-purity and low-molecular-weight chitosan (COS46). Compared with COST, COS46 is better at inhibiting CCl<sub>4</sub>-induced cell death, improving cell morphology, reducing ALT content, and improving cell antioxidant capacity. The effects of COST and COS46 on CCl<sub>4</sub>-induced acute liver injury were further verified in mice. Both COS46 and COST improved the appearance of the liver induced by CCl<sub>4</sub>, decreased the levels of ALT and AST in serum, and decreased the oxidation/antioxidant index in the liver. From the liver pathological section, the effect of COS46 was better. In addition, some indicators of COS46 showed a dose-dependent effect. In conclusion, compared with COST, low-molecular-weight COS46 has better antioxidant capacity and a better therapeutic effect on CCl<sub>4</sub>-induced acute liver injury.https://www.mdpi.com/1660-3397/22/3/128acute liver injuryCCl<sub>4</sub>chitosan oligosaccharideoxidation resistance |
| spellingShingle | Kai Wang Dawei Yu Yan Bai Hua Cao Jiao Guo Zhengquan Su Isolation and Purification of Chitosan Oligosaccharides (Mw ≤ 1000) and Their Protective Effect on Acute Liver Injury Caused by CCl<sub>4</sub> Marine Drugs acute liver injury CCl<sub>4</sub> chitosan oligosaccharide oxidation resistance |
| title | Isolation and Purification of Chitosan Oligosaccharides (Mw ≤ 1000) and Their Protective Effect on Acute Liver Injury Caused by CCl<sub>4</sub> |
| title_full | Isolation and Purification of Chitosan Oligosaccharides (Mw ≤ 1000) and Their Protective Effect on Acute Liver Injury Caused by CCl<sub>4</sub> |
| title_fullStr | Isolation and Purification of Chitosan Oligosaccharides (Mw ≤ 1000) and Their Protective Effect on Acute Liver Injury Caused by CCl<sub>4</sub> |
| title_full_unstemmed | Isolation and Purification of Chitosan Oligosaccharides (Mw ≤ 1000) and Their Protective Effect on Acute Liver Injury Caused by CCl<sub>4</sub> |
| title_short | Isolation and Purification of Chitosan Oligosaccharides (Mw ≤ 1000) and Their Protective Effect on Acute Liver Injury Caused by CCl<sub>4</sub> |
| title_sort | isolation and purification of chitosan oligosaccharides mw ≤ 1000 and their protective effect on acute liver injury caused by ccl sub 4 sub |
| topic | acute liver injury CCl<sub>4</sub> chitosan oligosaccharide oxidation resistance |
| url | https://www.mdpi.com/1660-3397/22/3/128 |
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