Studying the Gene Expressions of Bla OXA-51Like and Bla OXA-23Like in Acinetobacter baumannii
Genotypic detection of some Antibiotics Resistant genes by using polymerase chain reaction (PCR). (20) Isolates of Acinetobacter baumannii that showed resistance to (Ceftaxim, Cefotaxim, Cefepim and Imipenim) were selected. The results showed that 20 isolates of A. baumannii possess the bla...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Baghdad
2023-10-01
|
| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| Subjects: | |
| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3073 |
| _version_ | 1827787609467781120 |
|---|---|
| author | Rana Mujahid Abdullah Arkan Adnan Mahdi |
| author_facet | Rana Mujahid Abdullah Arkan Adnan Mahdi |
| author_sort | Rana Mujahid Abdullah |
| collection | DOAJ |
| description |
Genotypic detection of some Antibiotics Resistant genes by using polymerase chain reaction (PCR). (20) Isolates of Acinetobacter baumannii that showed resistance to (Ceftaxim, Cefotaxim, Cefepim and Imipenim) were selected. The results showed that 20 isolates of A. baumannii possess the bla-OXA23 like gene, and that all isolates possess this gene with a percentage (100%). With molecular weight 605 bp.
The current study showed that A. baumannii isolates carry 100% bla-OXA51like gene when studied with (20) isolates that are resistant to antibiotics (Imipenim Ceftazidime, Cifepime, Cifexime) that belong to this group of β-lactame with molecular weight 382 bp.
Gene expression was done of the bla-OXA23like and bla-OXA51like. The results of gene expression for bla-OXA23like gene show decrease the gene expression for bla-OXA23like gene when treated by Ceftazidim, whereas the bla-OXA51 like gene, showed increasing in gene expression when treated by Ceftazidime.
|
| first_indexed | 2024-03-11T16:51:22Z |
| format | Article |
| id | doaj.art-6d7e42e589df48a28ddf6495ae808003 |
| institution | Directory Open Access Journal |
| issn | 1609-4042 2521-3407 |
| language | English |
| last_indexed | 2024-03-11T16:51:22Z |
| publishDate | 2023-10-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj.art-6d7e42e589df48a28ddf6495ae8080032023-10-21T06:44:50ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072023-10-0136410.30526/36.4.3073Studying the Gene Expressions of Bla OXA-51Like and Bla OXA-23Like in Acinetobacter baumanniiRana Mujahid Abdullah 0Arkan Adnan Mahdi1Department of Biology, College of Education for Pure Science Ibn Al-Haitham, University of Baghdad, Baghdad, IraqDepartment of Biology, College of Education for Pure Science Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq Genotypic detection of some Antibiotics Resistant genes by using polymerase chain reaction (PCR). (20) Isolates of Acinetobacter baumannii that showed resistance to (Ceftaxim, Cefotaxim, Cefepim and Imipenim) were selected. The results showed that 20 isolates of A. baumannii possess the bla-OXA23 like gene, and that all isolates possess this gene with a percentage (100%). With molecular weight 605 bp. The current study showed that A. baumannii isolates carry 100% bla-OXA51like gene when studied with (20) isolates that are resistant to antibiotics (Imipenim Ceftazidime, Cifepime, Cifexime) that belong to this group of β-lactame with molecular weight 382 bp. Gene expression was done of the bla-OXA23like and bla-OXA51like. The results of gene expression for bla-OXA23like gene show decrease the gene expression for bla-OXA23like gene when treated by Ceftazidim, whereas the bla-OXA51 like gene, showed increasing in gene expression when treated by Ceftazidime. https://jih.uobaghdad.edu.iq/index.php/j/article/view/3073Key words:- Acinetobacter baumannii, resistance gene , gene expression |
| spellingShingle | Rana Mujahid Abdullah Arkan Adnan Mahdi Studying the Gene Expressions of Bla OXA-51Like and Bla OXA-23Like in Acinetobacter baumannii Ibn Al-Haitham Journal for Pure and Applied Sciences Key words:- Acinetobacter baumannii, resistance gene , gene expression |
| title | Studying the Gene Expressions of Bla OXA-51Like and Bla OXA-23Like in Acinetobacter baumannii |
| title_full | Studying the Gene Expressions of Bla OXA-51Like and Bla OXA-23Like in Acinetobacter baumannii |
| title_fullStr | Studying the Gene Expressions of Bla OXA-51Like and Bla OXA-23Like in Acinetobacter baumannii |
| title_full_unstemmed | Studying the Gene Expressions of Bla OXA-51Like and Bla OXA-23Like in Acinetobacter baumannii |
| title_short | Studying the Gene Expressions of Bla OXA-51Like and Bla OXA-23Like in Acinetobacter baumannii |
| title_sort | studying the gene expressions of bla oxa 51like and bla oxa 23like in acinetobacter baumannii |
| topic | Key words:- Acinetobacter baumannii, resistance gene , gene expression |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3073 |
| work_keys_str_mv | AT ranamujahidabdullah studyingthegeneexpressionsofblaoxa51likeandblaoxa23likeinacinetobacterbaumannii AT arkanadnanmahdi studyingthegeneexpressionsofblaoxa51likeandblaoxa23likeinacinetobacterbaumannii |