Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk
The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was pe...
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MDPI AG
2016-09-01
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Series: | Toxins |
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Online Access: | http://www.mdpi.com/2072-6651/8/9/268 |
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author | Yacine Nia Mélanie Rodriguez Reinhard Zeleny Sabine Herbin Frédéric Auvray Uwe Fiebig Marc-André Avondet Amalia Munoz Jacques-Antoine Hennekinne |
author_facet | Yacine Nia Mélanie Rodriguez Reinhard Zeleny Sabine Herbin Frédéric Auvray Uwe Fiebig Marc-André Avondet Amalia Munoz Jacques-Antoine Hennekinne |
author_sort | Yacine Nia |
collection | DOAJ |
description | The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was performed using SDS-PAGE and SE-specific ELISA, and amino acid analysis was used to assign its protein concentration. The solution was then used to prepare six PT materials (four milk and two buffer batches) at a ng/g toxin level, which included one blank and one SEA-containing milk as specificity control. Suitable material homogeneity and stability were assessed using screening and quantitative ELISAs. Among the methods used by the participants, ELISA-based methods demonstrated their efficiency for the detection of SEB in both simple and complex matrices. The results serve as a basis for further improving the detection capabilities in expert laboratories and can therefore be considered as a contribution to biopreparedness. |
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institution | Directory Open Access Journal |
issn | 2072-6651 |
language | English |
last_indexed | 2024-04-11T13:06:03Z |
publishDate | 2016-09-01 |
publisher | MDPI AG |
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series | Toxins |
spelling | doaj.art-6d7ef0ef85d44cd1b90efbb13dc749ae2022-12-22T04:22:46ZengMDPI AGToxins2072-66512016-09-018926810.3390/toxins8090268toxins8090268Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and MilkYacine Nia0Mélanie Rodriguez1Reinhard Zeleny2Sabine Herbin3Frédéric Auvray4Uwe Fiebig5Marc-André Avondet6Amalia Munoz7Jacques-Antoine Hennekinne8Laboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceLaboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceJoint Research Centre, Directorate for Health, Consumers and Reference Materials, European Commission, Retieseweg 111, Geel 2440, BelgiumLaboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceLaboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceCentre for Biological Threats and Special Pathogens Biological Toxins, Robert Koch Institute, Berlin 13353, GermanyLABOR SPIEZ, Eidgenössisches Departement für Verteidigung, Bevölkerungsschutz und Sport VBS Bundesamt für Bevölkerungsschutz BABS, Spiez 3700, SwitzerlandJoint Research Centre, Directorate for Health, Consumers and Reference Materials, European Commission, Retieseweg 111, Geel 2440, BelgiumLaboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceThe aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was performed using SDS-PAGE and SE-specific ELISA, and amino acid analysis was used to assign its protein concentration. The solution was then used to prepare six PT materials (four milk and two buffer batches) at a ng/g toxin level, which included one blank and one SEA-containing milk as specificity control. Suitable material homogeneity and stability were assessed using screening and quantitative ELISAs. Among the methods used by the participants, ELISA-based methods demonstrated their efficiency for the detection of SEB in both simple and complex matrices. The results serve as a basis for further improving the detection capabilities in expert laboratories and can therefore be considered as a contribution to biopreparedness.http://www.mdpi.com/2072-6651/8/9/268staphylococcal enterotoxin Bbioterrorism agentproficiency testELISA methods |
spellingShingle | Yacine Nia Mélanie Rodriguez Reinhard Zeleny Sabine Herbin Frédéric Auvray Uwe Fiebig Marc-André Avondet Amalia Munoz Jacques-Antoine Hennekinne Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk Toxins staphylococcal enterotoxin B bioterrorism agent proficiency test ELISA methods |
title | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_full | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_fullStr | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_full_unstemmed | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_short | Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk |
title_sort | organization and elisa based results of the first proficiency testing to evaluate the ability of european union laboratories to detect staphylococcal enterotoxin type b seb in buffer and milk |
topic | staphylococcal enterotoxin B bioterrorism agent proficiency test ELISA methods |
url | http://www.mdpi.com/2072-6651/8/9/268 |
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