Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers
Background and Objective: The healthy people’s fecal flora in the community represents a large potential reservoir. Therefore, the current study aimed to detect antibiotic resistance patterns and virulence factors in Klebsiella pneumoniae isolated from healthy volunteers’ feces. Materials and Method...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Farname
2021-12-01
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| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://ijmm.ir/article-1-1482-en.html |
| _version_ | 1819349381018550272 |
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| author | Amine Dalir Shabnam Razavi Malihe Talebi Faramarz Masjedian Jazi Abed Zahedi Bialvaei Maryam Mirshekar Vahid Lohrasbi |
| author_facet | Amine Dalir Shabnam Razavi Malihe Talebi Faramarz Masjedian Jazi Abed Zahedi Bialvaei Maryam Mirshekar Vahid Lohrasbi |
| author_sort | Amine Dalir |
| collection | DOAJ |
| description | Background and Objective: The healthy people’s fecal flora in the community represents a large potential reservoir. Therefore, the current study aimed to detect antibiotic resistance patterns and virulence factors in Klebsiella pneumoniae isolated from healthy volunteers’ feces.
Materials and Methods: Three hundred and fifty stool specimens were collected from sales rep healthy individuals referring to the Northwest Tehran Health Centers to get a health card. Bacterial isolation, identification, and antimicrobial susceptibility testing were conducted according to the routine instructions. In addition, polymerase chain reaction (PCR) was used to detect the genetic factors responsible for producing extended-spectrum β-lactamases (ESBLs: SHV, TEM, and CTX-M) blaKPC and other virulence genes.
Results: Among fecal samples analyzed, 60 (17.1%) K. pneumoniae were isolated. The results demonstrated that the highest resistance rate was related to piperacillin-tazobactam (n=25, 41.6%), followed by and meropenem (n=17, 28.8%) and co-trimoxazole (n=11, 18.3%), respectively. Also, all strains were susceptible to amikacin, gentamicin, and imipenem. The PCR results of the virulence gene showed that 95% (n=57) of isolates were positive for fimH gene, 93.33% (n=56) for BssS gene, 27 (45%) for rmpA gene. The PCR results for antibiotic resistance genes showed that 41.66% (n=25) had blaTEM gene, 38.33% (n=23) blaCTX-M gene, 35% (n=21) blaSHV gene and 3.33% (n=2) isolates had blaKPC gene, and none of these isolates carried magA gene.
Conclusion: Antibiotic resistance was common among K. pneumoniae isolated from healthy volunteers’ feces who participated in this study. Transmission of resistant bacteria and plasmids through oral-fecal sources threats to the public, which could complicate treatment options for community-acquired infections caused by K. pneumoniae. |
| first_indexed | 2024-12-24T18:59:37Z |
| format | Article |
| id | doaj.art-6d80fbe100d041acb4d62e1401f89b31 |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-12-24T18:59:37Z |
| publishDate | 2021-12-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-6d80fbe100d041acb4d62e1401f89b312022-12-21T16:43:15ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422021-12-01156676683Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy VolunteersAmine Dalir0Shabnam Razavi1Malihe Talebi2Faramarz Masjedian Jazi3Abed Zahedi Bialvaei4Maryam Mirshekar5Vahid Lohrasbi6 Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Background and Objective: The healthy people’s fecal flora in the community represents a large potential reservoir. Therefore, the current study aimed to detect antibiotic resistance patterns and virulence factors in Klebsiella pneumoniae isolated from healthy volunteers’ feces. Materials and Methods: Three hundred and fifty stool specimens were collected from sales rep healthy individuals referring to the Northwest Tehran Health Centers to get a health card. Bacterial isolation, identification, and antimicrobial susceptibility testing were conducted according to the routine instructions. In addition, polymerase chain reaction (PCR) was used to detect the genetic factors responsible for producing extended-spectrum β-lactamases (ESBLs: SHV, TEM, and CTX-M) blaKPC and other virulence genes. Results: Among fecal samples analyzed, 60 (17.1%) K. pneumoniae were isolated. The results demonstrated that the highest resistance rate was related to piperacillin-tazobactam (n=25, 41.6%), followed by and meropenem (n=17, 28.8%) and co-trimoxazole (n=11, 18.3%), respectively. Also, all strains were susceptible to amikacin, gentamicin, and imipenem. The PCR results of the virulence gene showed that 95% (n=57) of isolates were positive for fimH gene, 93.33% (n=56) for BssS gene, 27 (45%) for rmpA gene. The PCR results for antibiotic resistance genes showed that 41.66% (n=25) had blaTEM gene, 38.33% (n=23) blaCTX-M gene, 35% (n=21) blaSHV gene and 3.33% (n=2) isolates had blaKPC gene, and none of these isolates carried magA gene. Conclusion: Antibiotic resistance was common among K. pneumoniae isolated from healthy volunteers’ feces who participated in this study. Transmission of resistant bacteria and plasmids through oral-fecal sources threats to the public, which could complicate treatment options for community-acquired infections caused by K. pneumoniae.http://ijmm.ir/article-1-1482-en.htmlantibiotic resistanceesblklebsiella pneumoniaevirulence genes |
| spellingShingle | Amine Dalir Shabnam Razavi Malihe Talebi Faramarz Masjedian Jazi Abed Zahedi Bialvaei Maryam Mirshekar Vahid Lohrasbi Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers Iranian Journal of Medical Microbiology antibiotic resistance esbl klebsiella pneumoniae virulence genes |
| title | Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers |
| title_full | Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers |
| title_fullStr | Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers |
| title_full_unstemmed | Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers |
| title_short | Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers |
| title_sort | antibiotic susceptibility pattern and distribution of virulence factors among klebsiella pneumoniae isolated from healthy volunteers |
| topic | antibiotic resistance esbl klebsiella pneumoniae virulence genes |
| url | http://ijmm.ir/article-1-1482-en.html |
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