Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth.
We examined the thymoquinone induced inhibition of purified F1 or membrane bound F1FO E. coli ATP synthase. Both purified F1 and membrane bound F1FO were completely inhibited by thymoquinone with no residual ATPase activity. The process of inhibition was fully reversible and identical in both membra...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4440651?pdf=render |
| _version_ | 1818201065233842176 |
|---|---|
| author | Zulfiqar Ahmad Thomas F Laughlin Ismail O Kady |
| author_facet | Zulfiqar Ahmad Thomas F Laughlin Ismail O Kady |
| author_sort | Zulfiqar Ahmad |
| collection | DOAJ |
| description | We examined the thymoquinone induced inhibition of purified F1 or membrane bound F1FO E. coli ATP synthase. Both purified F1 and membrane bound F1FO were completely inhibited by thymoquinone with no residual ATPase activity. The process of inhibition was fully reversible and identical in both membrane bound F1Fo and purified F1 preparations. Moreover, thymoquinone induced inhibition of ATP synthase expressing wild-type E. coli cell growth and non-inhibition of ATPase gene deleted null control cells demonstrates that ATP synthase is a molecular target for thymoquinone. This also links the beneficial dietary based antimicrobial and anticancer effects of thymoquinone to its inhibitory action on ATP synthase. |
| first_indexed | 2024-12-12T02:47:37Z |
| format | Article |
| id | doaj.art-6d823fa5428244a9944b364a396bd2a1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T02:47:37Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6d823fa5428244a9944b364a396bd2a12022-12-22T00:40:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012780210.1371/journal.pone.0127802Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth.Zulfiqar AhmadThomas F LaughlinIsmail O KadyWe examined the thymoquinone induced inhibition of purified F1 or membrane bound F1FO E. coli ATP synthase. Both purified F1 and membrane bound F1FO were completely inhibited by thymoquinone with no residual ATPase activity. The process of inhibition was fully reversible and identical in both membrane bound F1Fo and purified F1 preparations. Moreover, thymoquinone induced inhibition of ATP synthase expressing wild-type E. coli cell growth and non-inhibition of ATPase gene deleted null control cells demonstrates that ATP synthase is a molecular target for thymoquinone. This also links the beneficial dietary based antimicrobial and anticancer effects of thymoquinone to its inhibitory action on ATP synthase.http://europepmc.org/articles/PMC4440651?pdf=render |
| spellingShingle | Zulfiqar Ahmad Thomas F Laughlin Ismail O Kady Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth. PLoS ONE |
| title | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth. |
| title_full | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth. |
| title_fullStr | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth. |
| title_full_unstemmed | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth. |
| title_short | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth. |
| title_sort | thymoquinone inhibits escherichia coli atp synthase and cell growth |
| url | http://europepmc.org/articles/PMC4440651?pdf=render |
| work_keys_str_mv | AT zulfiqarahmad thymoquinoneinhibitsescherichiacoliatpsynthaseandcellgrowth AT thomasflaughlin thymoquinoneinhibitsescherichiacoliatpsynthaseandcellgrowth AT ismailokady thymoquinoneinhibitsescherichiacoliatpsynthaseandcellgrowth |