Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium

Abstract Microorganisms play an essential role in sulfide removal. Alkaline absorption solution facilitates the sulfide’s dissolution and oxidative degradation, so haloalkaliphile is a prospective source for environmental-friendly and cost-effective biodesulfurization. In this research, 484 sulfide...

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Bibliographic Details
Main Authors: Manqi Zhang, Qiong Xue, Shengjie Zhang, Heng Zhou, Tong Xu, Jian Zhou, Yanning Zheng, Ming Li, Sumit Kumar, Dahe Zhao, Hua Xiang
Format: Article
Language:English
Published: SpringerOpen 2021-10-01
Series:AMB Express
Subjects:
Online Access:https://doi.org/10.1186/s13568-021-01302-9
Description
Summary:Abstract Microorganisms play an essential role in sulfide removal. Alkaline absorption solution facilitates the sulfide’s dissolution and oxidative degradation, so haloalkaliphile is a prospective source for environmental-friendly and cost-effective biodesulfurization. In this research, 484 sulfide oxidation genes were identified from the metagenomes of the soda-saline lakes and a haloalkaliphilic heterotrophic bacterium Halomonas salifodinae IM328 (=CGMCC 22183) was isolated from the same habitat as the host for expression of a representative sequence. The genetic manipulation was successfully achieved through the conjugation transformation method, and sulfide: quinone oxidoreductase gene (sqr) was expressed via pBBR1MCS derivative plasmid. Furthermore, a whole-cell catalyst system was developed by using the engineered strain that exhibited a higher rate of sulfide oxidation under the optimal alkaline pH of 9.0. The whole-cell catalyst could be recycled six times to maintain the sulfide oxidation rates from 41.451 to 80.216 µmol·min−1·g−1 dry cell mass. To summarize, a whole-cell catalyst system based on the engineered haloalkaliphilic bacterium is potentiated to be applied in the sulfide treatment at a reduced cost.
ISSN:2191-0855