Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium
Abstract Microorganisms play an essential role in sulfide removal. Alkaline absorption solution facilitates the sulfide’s dissolution and oxidative degradation, so haloalkaliphile is a prospective source for environmental-friendly and cost-effective biodesulfurization. In this research, 484 sulfide...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-10-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-021-01302-9 |
| _version_ | 1819003823077720064 |
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| author | Manqi Zhang Qiong Xue Shengjie Zhang Heng Zhou Tong Xu Jian Zhou Yanning Zheng Ming Li Sumit Kumar Dahe Zhao Hua Xiang |
| author_facet | Manqi Zhang Qiong Xue Shengjie Zhang Heng Zhou Tong Xu Jian Zhou Yanning Zheng Ming Li Sumit Kumar Dahe Zhao Hua Xiang |
| author_sort | Manqi Zhang |
| collection | DOAJ |
| description | Abstract Microorganisms play an essential role in sulfide removal. Alkaline absorption solution facilitates the sulfide’s dissolution and oxidative degradation, so haloalkaliphile is a prospective source for environmental-friendly and cost-effective biodesulfurization. In this research, 484 sulfide oxidation genes were identified from the metagenomes of the soda-saline lakes and a haloalkaliphilic heterotrophic bacterium Halomonas salifodinae IM328 (=CGMCC 22183) was isolated from the same habitat as the host for expression of a representative sequence. The genetic manipulation was successfully achieved through the conjugation transformation method, and sulfide: quinone oxidoreductase gene (sqr) was expressed via pBBR1MCS derivative plasmid. Furthermore, a whole-cell catalyst system was developed by using the engineered strain that exhibited a higher rate of sulfide oxidation under the optimal alkaline pH of 9.0. The whole-cell catalyst could be recycled six times to maintain the sulfide oxidation rates from 41.451 to 80.216 µmol·min−1·g−1 dry cell mass. To summarize, a whole-cell catalyst system based on the engineered haloalkaliphilic bacterium is potentiated to be applied in the sulfide treatment at a reduced cost. |
| first_indexed | 2024-12-20T23:27:07Z |
| format | Article |
| id | doaj.art-6d88f033647242188477387bb0be185b |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-20T23:27:07Z |
| publishDate | 2021-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-6d88f033647242188477387bb0be185b2022-12-21T19:23:22ZengSpringerOpenAMB Express2191-08552021-10-0111111410.1186/s13568-021-01302-9Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacteriumManqi Zhang0Qiong Xue1Shengjie Zhang2Heng Zhou3Tong Xu4Jian Zhou5Yanning Zheng6Ming Li7Sumit Kumar8Dahe Zhao9Hua Xiang10State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesEnzyme and Microbial Biochemistry Lab, Department of Chemistry, Indian Institute of TechnologyState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesAbstract Microorganisms play an essential role in sulfide removal. Alkaline absorption solution facilitates the sulfide’s dissolution and oxidative degradation, so haloalkaliphile is a prospective source for environmental-friendly and cost-effective biodesulfurization. In this research, 484 sulfide oxidation genes were identified from the metagenomes of the soda-saline lakes and a haloalkaliphilic heterotrophic bacterium Halomonas salifodinae IM328 (=CGMCC 22183) was isolated from the same habitat as the host for expression of a representative sequence. The genetic manipulation was successfully achieved through the conjugation transformation method, and sulfide: quinone oxidoreductase gene (sqr) was expressed via pBBR1MCS derivative plasmid. Furthermore, a whole-cell catalyst system was developed by using the engineered strain that exhibited a higher rate of sulfide oxidation under the optimal alkaline pH of 9.0. The whole-cell catalyst could be recycled six times to maintain the sulfide oxidation rates from 41.451 to 80.216 µmol·min−1·g−1 dry cell mass. To summarize, a whole-cell catalyst system based on the engineered haloalkaliphilic bacterium is potentiated to be applied in the sulfide treatment at a reduced cost.https://doi.org/10.1186/s13568-021-01302-9Hydrogen sulfide treatmentSulfide: quinone oxidoreductaseHaloalkaliphilic heterotrophic bacteriumGenetic modificationWhole-cell catalysisProcess optimization |
| spellingShingle | Manqi Zhang Qiong Xue Shengjie Zhang Heng Zhou Tong Xu Jian Zhou Yanning Zheng Ming Li Sumit Kumar Dahe Zhao Hua Xiang Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium AMB Express Hydrogen sulfide treatment Sulfide: quinone oxidoreductase Haloalkaliphilic heterotrophic bacterium Genetic modification Whole-cell catalysis Process optimization |
| title | Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium |
| title_full | Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium |
| title_fullStr | Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium |
| title_full_unstemmed | Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium |
| title_short | Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium |
| title_sort | development of whole cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium |
| topic | Hydrogen sulfide treatment Sulfide: quinone oxidoreductase Haloalkaliphilic heterotrophic bacterium Genetic modification Whole-cell catalysis Process optimization |
| url | https://doi.org/10.1186/s13568-021-01302-9 |
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