Value of urinary topoisomerase-IIA cell-free DNA for diagnosis of bladder cancer
Purpose: Topoisomerase-II alpha (TopoIIA ), a DNA gyrase isoform that plays an important role in the cell cycle, is present in normal tissues and various human cancers, and can show altered expression in both. The aim of the current study was to examine the value of urinary TopoIIA cell-free DNA a...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Korean Urological Association
2016-03-01
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| Series: | Investigative and Clinical Urology |
| Subjects: | |
| Online Access: | https://www.icurology.org/Synapse/Data/PDFData/2020ICU/icu-57-106.pdf |
| _version_ | 1818278792957788160 |
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| author | Ye-Hwan Kim Chunri Yan Il-Seok Lee Xuan-Mei Piao Young Joon Byun Pildu Jeong Won Tae Kim Seok-Joong Yun Wun-Jae Kim |
| author_facet | Ye-Hwan Kim Chunri Yan Il-Seok Lee Xuan-Mei Piao Young Joon Byun Pildu Jeong Won Tae Kim Seok-Joong Yun Wun-Jae Kim |
| author_sort | Ye-Hwan Kim |
| collection | DOAJ |
| description | Purpose: Topoisomerase-II alpha (TopoIIA ), a DNA gyrase isoform that plays an important role in the cell cycle, is present in normal
tissues and various human cancers, and can show altered expression in both. The aim of the current study was to examine the
value of urinary TopoIIA cell-free DNA as a noninvasive diagnosis of bladder cancer (BC).
Materials and Methods: Two patient cohorts were examined. Cohort 1 (73 BC patients and seven controls) provided bladder tissue
samples, whereas cohort 2 (83 BC patients, 54 nonmalignant hematuric patients, and 61 normal controls) provided urine samples.
Real-time quantitative polymerase chain reaction was used to measure expression of TopoIIA mRNA in tissues and TopoIIA
cell-free DNA in urine samples.
Results: The results showed that expression of TopoIIA mRNA in BC tissues was significantly higher than that in noncancer control
tissues (p<0.001). The expression of urinary TopoIIA cell-free DNA in BC patients was also significantly higher than that in noncancer
patient controls and hematuria patients (p < 0.001 and p < 0.001, respectively). High expression of urinary TopoIIA cell-free
DNA was also detected in muscle invasive bladder cancer (MIBC) when compared with nonmuscle invasive bladder cancer (NMIBC)
(p=0.002). Receiver operating characteristics (ROC) curve analysis was performed to examine the sensitivity/specificity of urinary
TopoIIA cell-free DNA for diagnosing BC, NMIBC, and MIBC. The areas under the ROC curve for BC, NMIBC, and MIBC were 0.741,
0.701, and 0.838, respectively.
Conclusions: In summary, the results of this study provide evidence that cell-free TopoIIA DNA may be a potential biomarker for
BC. |
| first_indexed | 2024-12-12T23:23:04Z |
| format | Article |
| id | doaj.art-6d894d6cee2044d3ae9ce28ee73a2f30 |
| institution | Directory Open Access Journal |
| issn | 2466-0493 2466-054X |
| language | English |
| last_indexed | 2024-12-12T23:23:04Z |
| publishDate | 2016-03-01 |
| publisher | Korean Urological Association |
| record_format | Article |
| series | Investigative and Clinical Urology |
| spelling | doaj.art-6d894d6cee2044d3ae9ce28ee73a2f302022-12-22T00:08:13ZengKorean Urological AssociationInvestigative and Clinical Urology2466-04932466-054X2016-03-0157210611210.4111/icu.2016.57.2.106Value of urinary topoisomerase-IIA cell-free DNA for diagnosis of bladder cancerYe-Hwan Kim0Chunri Yan1Il-Seok Lee2Xuan-Mei Piao3Young Joon Byun4Pildu Jeong5Won Tae Kim6Seok-Joong Yun7Wun-Jae Kim8Chungbuk National University College of MedicineChungbuk National University College of MedicineChungbuk National University College of MedicineChungbuk National University College of MedicineChungbuk National University College of MedicineChungbuk National University College of MedicineChungbuk National University College of MedicineChungbuk National University College of MedicineChungbuk National University College of MedicinePurpose: Topoisomerase-II alpha (TopoIIA ), a DNA gyrase isoform that plays an important role in the cell cycle, is present in normal tissues and various human cancers, and can show altered expression in both. The aim of the current study was to examine the value of urinary TopoIIA cell-free DNA as a noninvasive diagnosis of bladder cancer (BC). Materials and Methods: Two patient cohorts were examined. Cohort 1 (73 BC patients and seven controls) provided bladder tissue samples, whereas cohort 2 (83 BC patients, 54 nonmalignant hematuric patients, and 61 normal controls) provided urine samples. Real-time quantitative polymerase chain reaction was used to measure expression of TopoIIA mRNA in tissues and TopoIIA cell-free DNA in urine samples. Results: The results showed that expression of TopoIIA mRNA in BC tissues was significantly higher than that in noncancer control tissues (p<0.001). The expression of urinary TopoIIA cell-free DNA in BC patients was also significantly higher than that in noncancer patient controls and hematuria patients (p < 0.001 and p < 0.001, respectively). High expression of urinary TopoIIA cell-free DNA was also detected in muscle invasive bladder cancer (MIBC) when compared with nonmuscle invasive bladder cancer (NMIBC) (p=0.002). Receiver operating characteristics (ROC) curve analysis was performed to examine the sensitivity/specificity of urinary TopoIIA cell-free DNA for diagnosing BC, NMIBC, and MIBC. The areas under the ROC curve for BC, NMIBC, and MIBC were 0.741, 0.701, and 0.838, respectively. Conclusions: In summary, the results of this study provide evidence that cell-free TopoIIA DNA may be a potential biomarker for BC.https://www.icurology.org/Synapse/Data/PDFData/2020ICU/icu-57-106.pdfBiomarkers; DNA topoisomerase II alpha; Hematuria; Urinary bladder neoplasms; Urine |
| spellingShingle | Ye-Hwan Kim Chunri Yan Il-Seok Lee Xuan-Mei Piao Young Joon Byun Pildu Jeong Won Tae Kim Seok-Joong Yun Wun-Jae Kim Value of urinary topoisomerase-IIA cell-free DNA for diagnosis of bladder cancer Investigative and Clinical Urology Biomarkers; DNA topoisomerase II alpha; Hematuria; Urinary bladder neoplasms; Urine |
| title | Value of urinary topoisomerase-IIA cell-free DNA for diagnosis of bladder cancer |
| title_full | Value of urinary topoisomerase-IIA cell-free DNA for diagnosis of bladder cancer |
| title_fullStr | Value of urinary topoisomerase-IIA cell-free DNA for diagnosis of bladder cancer |
| title_full_unstemmed | Value of urinary topoisomerase-IIA cell-free DNA for diagnosis of bladder cancer |
| title_short | Value of urinary topoisomerase-IIA cell-free DNA for diagnosis of bladder cancer |
| title_sort | value of urinary topoisomerase iia cell free dna for diagnosis of bladder cancer |
| topic | Biomarkers; DNA topoisomerase II alpha; Hematuria; Urinary bladder neoplasms; Urine |
| url | https://www.icurology.org/Synapse/Data/PDFData/2020ICU/icu-57-106.pdf |
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