Discovery of exolytic heparinases and their catalytic mechanism and potential application
Exolytic heparinases are needed for sequencing of heparin and heparan sulfate (HP), but have not yet been reported. Here, the authors identify exolytic heparinases from different bacteria and show that the heparinases preferentially digest HP, determine the crystal structure of the exoheparinase Ble...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-21441-8 |
| _version_ | 1819202162464391168 |
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| author | Qingdong Zhang Hai-Yan Cao Lin Wei Danrong Lu Min Du Min Yuan Deling Shi Xiangxue Chen Peng Wang Xiu-Lan Chen Lianli Chi Yu-Zhong Zhang Fuchuan Li |
| author_facet | Qingdong Zhang Hai-Yan Cao Lin Wei Danrong Lu Min Du Min Yuan Deling Shi Xiangxue Chen Peng Wang Xiu-Lan Chen Lianli Chi Yu-Zhong Zhang Fuchuan Li |
| author_sort | Qingdong Zhang |
| collection | DOAJ |
| description | Exolytic heparinases are needed for sequencing of heparin and heparan sulfate (HP), but have not yet been reported. Here, the authors identify exolytic heparinases from different bacteria and show that the heparinases preferentially digest HP, determine the crystal structure of the exoheparinase BlexoHep and perform sequencing of HP octasaccharides using the enzyme. |
| first_indexed | 2024-12-23T03:59:38Z |
| format | Article |
| id | doaj.art-6d8a8b43feb24d34a0e24a72e67a1fa4 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-23T03:59:38Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-6d8a8b43feb24d34a0e24a72e67a1fa42022-12-21T18:00:45ZengNature PortfolioNature Communications2041-17232021-02-0112111310.1038/s41467-021-21441-8Discovery of exolytic heparinases and their catalytic mechanism and potential applicationQingdong Zhang0Hai-Yan Cao1Lin Wei2Danrong Lu3Min Du4Min Yuan5Deling Shi6Xiangxue Chen7Peng Wang8Xiu-Lan Chen9Lianli Chi10Yu-Zhong Zhang11Fuchuan Li12National Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong UniversityNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong UniversityNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong UniversityNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong UniversityNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong UniversityTiandong PharmaCollege of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of ChinaState Key Laboratory of Microbial Technology, Shandong UniversityNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong UniversityCollege of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of ChinaNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong UniversityExolytic heparinases are needed for sequencing of heparin and heparan sulfate (HP), but have not yet been reported. Here, the authors identify exolytic heparinases from different bacteria and show that the heparinases preferentially digest HP, determine the crystal structure of the exoheparinase BlexoHep and perform sequencing of HP octasaccharides using the enzyme.https://doi.org/10.1038/s41467-021-21441-8 |
| spellingShingle | Qingdong Zhang Hai-Yan Cao Lin Wei Danrong Lu Min Du Min Yuan Deling Shi Xiangxue Chen Peng Wang Xiu-Lan Chen Lianli Chi Yu-Zhong Zhang Fuchuan Li Discovery of exolytic heparinases and their catalytic mechanism and potential application Nature Communications |
| title | Discovery of exolytic heparinases and their catalytic mechanism and potential application |
| title_full | Discovery of exolytic heparinases and their catalytic mechanism and potential application |
| title_fullStr | Discovery of exolytic heparinases and their catalytic mechanism and potential application |
| title_full_unstemmed | Discovery of exolytic heparinases and their catalytic mechanism and potential application |
| title_short | Discovery of exolytic heparinases and their catalytic mechanism and potential application |
| title_sort | discovery of exolytic heparinases and their catalytic mechanism and potential application |
| url | https://doi.org/10.1038/s41467-021-21441-8 |
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