Microbial Community Structure and Function Succession of Puffer Fish (Takifugu obscurus) During Refrigeration
Objective: To reveal the microbial community and its function succession principles of puffer fish (Takifugu obscurus), and to identify the dominant microbial genera on puffer fish during refrigerated storage. Methods: High-throughput sequencing was employed to investigate the microbial composition...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-07-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100165 |
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| author | Longfei YAN Wenjing YAN Fanwei DAI Jiahong LI Yulong CHEN Feiping CHEN Ling WANG Zheng LUO Mingqiang YE Jianhao ZHANG |
| author_facet | Longfei YAN Wenjing YAN Fanwei DAI Jiahong LI Yulong CHEN Feiping CHEN Ling WANG Zheng LUO Mingqiang YE Jianhao ZHANG |
| author_sort | Longfei YAN |
| collection | DOAJ |
| description | Objective: To reveal the microbial community and its function succession principles of puffer fish (Takifugu obscurus), and to identify the dominant microbial genera on puffer fish during refrigerated storage. Methods: High-throughput sequencing was employed to investigate the microbial composition of puffer fish during refrigeration (4 ℃, 8 d), the community function was predicted by Tax4fun2, and the total volatile basic-nitrogen (TVB-N), pH and organoleptic values of puffer fish were measured as well. Results: The high-throughput sequencing demonstrated that during refrigeration the bacterial community of puffer fish succeed significantly, which were mainly composed of genera Acinetobacter, Lactobacillus, Aeromonas and Pseudomonas at the initial stage, while at the last stage composed almost of genera Pseudomonas (>91.7%), which was considered as the dominant genera on puffer fish. The α-diversity index of the community was higher at initial stage, while decreased during the storage. The microbial community function prediction results showed that the relative abundance of the amino-acid metabolism pathway and the cellular communication pathway increased at the last stage of the storage. As for the physicochemical properties, TVB-N, pH and total microbial counts increased while sensory scores decreased during the storage. Conclusion: The microbial community and its functional profile changed significantly during refrigeration, Pseudomonas was the dominant genera of puffer fish, and the above conclusion would provide theoretic guidelines for developing novel preserving technology for puffer fish. |
| first_indexed | 2024-03-12T13:34:23Z |
| format | Article |
| id | doaj.art-6d8c880e574d4422961be8df85993e8c |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:23Z |
| publishDate | 2023-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-6d8c880e574d4422961be8df85993e8c2023-08-24T06:00:30ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-07-01441436336910.13386/j.issn1002-0306.20221001652022100165-14Microbial Community Structure and Function Succession of Puffer Fish (Takifugu obscurus) During RefrigerationLongfei YAN0Wenjing YAN1Fanwei DAI2Jiahong LI3Yulong CHEN4Feiping CHEN5Ling WANG6Zheng LUO7Mingqiang YE8Jianhao ZHANG9College of Foods Science and Technology, Nanjing Agriculture University, Nanjing 210095, ChinaCollege of Foods Science and Technology, Nanjing Agriculture University, Nanjing 210095, ChinaSericulture & Agri-Food Research Institute Guangdong Academy of Agriculture Science, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericulture & Agri-Food Research Institute Guangdong Academy of Agriculture Science, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericulture & Agri-Food Research Institute Guangdong Academy of Agriculture Science, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericulture & Agri-Food Research Institute Guangdong Academy of Agriculture Science, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericulture & Agri-Food Research Institute Guangdong Academy of Agriculture Science, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericulture & Agri-Food Research Institute Guangdong Academy of Agriculture Science, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericulture & Agri-Food Research Institute Guangdong Academy of Agriculture Science, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaCollege of Foods Science and Technology, Nanjing Agriculture University, Nanjing 210095, ChinaObjective: To reveal the microbial community and its function succession principles of puffer fish (Takifugu obscurus), and to identify the dominant microbial genera on puffer fish during refrigerated storage. Methods: High-throughput sequencing was employed to investigate the microbial composition of puffer fish during refrigeration (4 ℃, 8 d), the community function was predicted by Tax4fun2, and the total volatile basic-nitrogen (TVB-N), pH and organoleptic values of puffer fish were measured as well. Results: The high-throughput sequencing demonstrated that during refrigeration the bacterial community of puffer fish succeed significantly, which were mainly composed of genera Acinetobacter, Lactobacillus, Aeromonas and Pseudomonas at the initial stage, while at the last stage composed almost of genera Pseudomonas (>91.7%), which was considered as the dominant genera on puffer fish. The α-diversity index of the community was higher at initial stage, while decreased during the storage. The microbial community function prediction results showed that the relative abundance of the amino-acid metabolism pathway and the cellular communication pathway increased at the last stage of the storage. As for the physicochemical properties, TVB-N, pH and total microbial counts increased while sensory scores decreased during the storage. Conclusion: The microbial community and its functional profile changed significantly during refrigeration, Pseudomonas was the dominant genera of puffer fish, and the above conclusion would provide theoretic guidelines for developing novel preserving technology for puffer fish.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100165puffer fish (takifugu obscurus)high-throughput sequencingpseudomonasspoilagerefrigerated preserving |
| spellingShingle | Longfei YAN Wenjing YAN Fanwei DAI Jiahong LI Yulong CHEN Feiping CHEN Ling WANG Zheng LUO Mingqiang YE Jianhao ZHANG Microbial Community Structure and Function Succession of Puffer Fish (Takifugu obscurus) During Refrigeration Shipin gongye ke-ji puffer fish (takifugu obscurus) high-throughput sequencing pseudomonas spoilage refrigerated preserving |
| title | Microbial Community Structure and Function Succession of Puffer Fish (Takifugu obscurus) During Refrigeration |
| title_full | Microbial Community Structure and Function Succession of Puffer Fish (Takifugu obscurus) During Refrigeration |
| title_fullStr | Microbial Community Structure and Function Succession of Puffer Fish (Takifugu obscurus) During Refrigeration |
| title_full_unstemmed | Microbial Community Structure and Function Succession of Puffer Fish (Takifugu obscurus) During Refrigeration |
| title_short | Microbial Community Structure and Function Succession of Puffer Fish (Takifugu obscurus) During Refrigeration |
| title_sort | microbial community structure and function succession of puffer fish takifugu obscurus during refrigeration |
| topic | puffer fish (takifugu obscurus) high-throughput sequencing pseudomonas spoilage refrigerated preserving |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100165 |
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