Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C
New nutrient media based on baker yeast autolizate were used for the first time for manufacturing of diagnostic preparation of plague bacteriophage L-413C. Experimental media provide high concentration of phage particles at the stage of propagation, and good survivability in lyophilization. Media in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2011-04-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/756 |
| _version_ | 1797220947365724160 |
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| author | T. V. Alenkina O. S. Zinina M. V. Antonycheva N. I. Vakhrushina A. K. Nikiforov |
| author_facet | T. V. Alenkina O. S. Zinina M. V. Antonycheva N. I. Vakhrushina A. K. Nikiforov |
| author_sort | T. V. Alenkina |
| collection | DOAJ |
| description | New nutrient media based on baker yeast autolizate were used for the first time for manufacturing of diagnostic preparation of plague bacteriophage L-413C. Experimental media provide high concentration of phage particles at the stage of propagation, and good survivability in lyophilization. Media in which yeast autolizate was a nutrient protein basis appeared to be more effective than those in which it was a stimulating additive. Phage preparations preserved stability of properties during storage at 4-8 °С, and at a higher temperature in the test of accelerated aging. Introduction of yeast nutrient media in technology of plague diagnostic bacteriophage L-413C manufacturing opens good prospects for increasing of production efficiency and decreasing of cost value of the preparation |
| first_indexed | 2024-03-08T13:44:29Z |
| format | Article |
| id | doaj.art-6d8d8a4e7fa2480a92766abb854f813e |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:57:38Z |
| publishDate | 2011-04-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-6d8d8a4e7fa2480a92766abb854f813e2024-04-05T16:47:07ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2011-04-0102(108)798210.21055/0370-1069-2011-2(108)-79-82740Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413CT. V. Alenkina0O. S. Zinina1M. V. Antonycheva2N. I. Vakhrushina3A. K. Nikiforov4Russian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeRussian Research Anti-Plague Institute MicrobeNew nutrient media based on baker yeast autolizate were used for the first time for manufacturing of diagnostic preparation of plague bacteriophage L-413C. Experimental media provide high concentration of phage particles at the stage of propagation, and good survivability in lyophilization. Media in which yeast autolizate was a nutrient protein basis appeared to be more effective than those in which it was a stimulating additive. Phage preparations preserved stability of properties during storage at 4-8 °С, and at a higher temperature in the test of accelerated aging. Introduction of yeast nutrient media in technology of plague diagnostic bacteriophage L-413C manufacturing opens good prospects for increasing of production efficiency and decreasing of cost value of the preparationhttps://journal.microbe.ru/jour/article/view/756diagnostic bacteriophagesplague bacteriophage l-413cbaker yeasts autolizatepropagation of phages |
| spellingShingle | T. V. Alenkina O. S. Zinina M. V. Antonycheva N. I. Vakhrushina A. K. Nikiforov Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C Проблемы особо опасных инфекций diagnostic bacteriophages plague bacteriophage l-413c baker yeasts autolizate propagation of phages |
| title | Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C |
| title_full | Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C |
| title_fullStr | Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C |
| title_full_unstemmed | Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C |
| title_short | Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C |
| title_sort | optimization of reproduction stage in technology of production of plague diagnostic bacteriophage l 413c |
| topic | diagnostic bacteriophages plague bacteriophage l-413c baker yeasts autolizate propagation of phages |
| url | https://journal.microbe.ru/jour/article/view/756 |
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