Intrathecal injection of spironolactone attenuates radicular pain by inhibition of spinal microglia activation in a rat model.
Microglia might play an important role in nociceptive processing and hyperalgesia by neuroinflammatory process. Mineralocorticoid receptor (MR) expressed on microglia might play a central role in the modulation of microglia activity. However the roles of microglia and MR in radicular pain were not w...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3387194?pdf=render |
| _version_ | 1818063298625536000 |
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| author | Yu-e Sun Liangyu Peng Xiaofeng Sun Jinhua Bo Dong Yang Yaguo Zheng Chenglong Liu Beibei Zhu Zhengliang Ma Xiaoping Gu |
| author_facet | Yu-e Sun Liangyu Peng Xiaofeng Sun Jinhua Bo Dong Yang Yaguo Zheng Chenglong Liu Beibei Zhu Zhengliang Ma Xiaoping Gu |
| author_sort | Yu-e Sun |
| collection | DOAJ |
| description | Microglia might play an important role in nociceptive processing and hyperalgesia by neuroinflammatory process. Mineralocorticoid receptor (MR) expressed on microglia might play a central role in the modulation of microglia activity. However the roles of microglia and MR in radicular pain were not well understood. This study sought to investigate whether selective MR antagonist spironolactone develop antinociceptive effects on radicular pain by inhibition neuroinflammation induced by spinal microglia activation.Radicular pain was produced by chronic compression of the dorsal root ganglia with SURGIFLO™. The expression of microglia, interleukin beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), NR1 subunit of the NMDA receptor (t-NR1), and NR1 subunit phosphorylated at Ser896 (p-NR1) were also markedly up-regulated. Intrathecal injection of spironolactone significantly attenuated pain behaviors as well as the expression of microglia, IL-1β, TNF-α, t-NR1, and p-NR1, whereas the production of IL-6 wasn't affected.These results suggest that intrathecal delivery spironolactone has therapeutic effects on radicular pain in rats. Decreasing the activation of glial cells, the production of proinflammatory cytokines and down-regulating the expression and phosphorylation of NMDA receptors in the spinal dorsal horn and dorsal root ganglia are the main mechanisms contributing to its beneficial effects. |
| first_indexed | 2024-12-10T14:17:53Z |
| format | Article |
| id | doaj.art-6d92de30c73f491b9cc33e9773ba14c8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T14:17:53Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6d92de30c73f491b9cc33e9773ba14c82022-12-22T01:45:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3989710.1371/journal.pone.0039897Intrathecal injection of spironolactone attenuates radicular pain by inhibition of spinal microglia activation in a rat model.Yu-e SunLiangyu PengXiaofeng SunJinhua BoDong YangYaguo ZhengChenglong LiuBeibei ZhuZhengliang MaXiaoping GuMicroglia might play an important role in nociceptive processing and hyperalgesia by neuroinflammatory process. Mineralocorticoid receptor (MR) expressed on microglia might play a central role in the modulation of microglia activity. However the roles of microglia and MR in radicular pain were not well understood. This study sought to investigate whether selective MR antagonist spironolactone develop antinociceptive effects on radicular pain by inhibition neuroinflammation induced by spinal microglia activation.Radicular pain was produced by chronic compression of the dorsal root ganglia with SURGIFLO™. The expression of microglia, interleukin beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), NR1 subunit of the NMDA receptor (t-NR1), and NR1 subunit phosphorylated at Ser896 (p-NR1) were also markedly up-regulated. Intrathecal injection of spironolactone significantly attenuated pain behaviors as well as the expression of microglia, IL-1β, TNF-α, t-NR1, and p-NR1, whereas the production of IL-6 wasn't affected.These results suggest that intrathecal delivery spironolactone has therapeutic effects on radicular pain in rats. Decreasing the activation of glial cells, the production of proinflammatory cytokines and down-regulating the expression and phosphorylation of NMDA receptors in the spinal dorsal horn and dorsal root ganglia are the main mechanisms contributing to its beneficial effects.http://europepmc.org/articles/PMC3387194?pdf=render |
| spellingShingle | Yu-e Sun Liangyu Peng Xiaofeng Sun Jinhua Bo Dong Yang Yaguo Zheng Chenglong Liu Beibei Zhu Zhengliang Ma Xiaoping Gu Intrathecal injection of spironolactone attenuates radicular pain by inhibition of spinal microglia activation in a rat model. PLoS ONE |
| title | Intrathecal injection of spironolactone attenuates radicular pain by inhibition of spinal microglia activation in a rat model. |
| title_full | Intrathecal injection of spironolactone attenuates radicular pain by inhibition of spinal microglia activation in a rat model. |
| title_fullStr | Intrathecal injection of spironolactone attenuates radicular pain by inhibition of spinal microglia activation in a rat model. |
| title_full_unstemmed | Intrathecal injection of spironolactone attenuates radicular pain by inhibition of spinal microglia activation in a rat model. |
| title_short | Intrathecal injection of spironolactone attenuates radicular pain by inhibition of spinal microglia activation in a rat model. |
| title_sort | intrathecal injection of spironolactone attenuates radicular pain by inhibition of spinal microglia activation in a rat model |
| url | http://europepmc.org/articles/PMC3387194?pdf=render |
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