High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip
Single-cell proteomics is an emerging technology but protein coverage, throughput and quantitation accuracy are often still insufficient. Here, the authors develop a nested nanowell chip that improves protein recovery, throughput and robustness of isobaric labeling-based quantitative single-cell pro...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-26514-2 |
| _version_ | 1819024895356436480 |
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| author | Jongmin Woo Sarah M. Williams Lye Meng Markillie Song Feng Chai-Feng Tsai Victor Aguilera-Vazquez Ryan L. Sontag Ronald J. Moore Dehong Hu Hardeep S. Mehta Joshua Cantlon-Bruce Tao Liu Joshua N. Adkins Richard D. Smith Geremy C. Clair Ljiljana Pasa-Tolic Ying Zhu |
| author_facet | Jongmin Woo Sarah M. Williams Lye Meng Markillie Song Feng Chai-Feng Tsai Victor Aguilera-Vazquez Ryan L. Sontag Ronald J. Moore Dehong Hu Hardeep S. Mehta Joshua Cantlon-Bruce Tao Liu Joshua N. Adkins Richard D. Smith Geremy C. Clair Ljiljana Pasa-Tolic Ying Zhu |
| author_sort | Jongmin Woo |
| collection | DOAJ |
| description | Single-cell proteomics is an emerging technology but protein coverage, throughput and quantitation accuracy are often still insufficient. Here, the authors develop a nested nanowell chip that improves protein recovery, throughput and robustness of isobaric labeling-based quantitative single-cell proteomics. |
| first_indexed | 2024-12-21T05:02:03Z |
| format | Article |
| id | doaj.art-6d95da1c95fe4f8f92f03a0bae481111 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-21T05:02:03Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-6d95da1c95fe4f8f92f03a0bae4811112022-12-21T19:15:13ZengNature PortfolioNature Communications2041-17232021-10-0112111110.1038/s41467-021-26514-2High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chipJongmin Woo0Sarah M. Williams1Lye Meng Markillie2Song Feng3Chai-Feng Tsai4Victor Aguilera-Vazquez5Ryan L. Sontag6Ronald J. Moore7Dehong Hu8Hardeep S. Mehta9Joshua Cantlon-Bruce10Tao Liu11Joshua N. Adkins12Richard D. Smith13Geremy C. Clair14Ljiljana Pasa-Tolic15Ying Zhu16Environmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryEnvironmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryEnvironmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryEnvironmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryEnvironmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryEnvironmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryScienion AGBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryEnvironmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryEnvironmental Molecular Sciences Laboratory, Pacific Northwest National LaboratorySingle-cell proteomics is an emerging technology but protein coverage, throughput and quantitation accuracy are often still insufficient. Here, the authors develop a nested nanowell chip that improves protein recovery, throughput and robustness of isobaric labeling-based quantitative single-cell proteomics.https://doi.org/10.1038/s41467-021-26514-2 |
| spellingShingle | Jongmin Woo Sarah M. Williams Lye Meng Markillie Song Feng Chai-Feng Tsai Victor Aguilera-Vazquez Ryan L. Sontag Ronald J. Moore Dehong Hu Hardeep S. Mehta Joshua Cantlon-Bruce Tao Liu Joshua N. Adkins Richard D. Smith Geremy C. Clair Ljiljana Pasa-Tolic Ying Zhu High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip Nature Communications |
| title | High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip |
| title_full | High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip |
| title_fullStr | High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip |
| title_full_unstemmed | High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip |
| title_short | High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip |
| title_sort | high throughput and high efficiency sample preparation for single cell proteomics using a nested nanowell chip |
| url | https://doi.org/10.1038/s41467-021-26514-2 |
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