H2O2-sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assay
Photoacoustic (PA) imaging with much deeper tissue penetration and better spatial resolution had been widely employed for the prevention and diagnosis of many diseases. In this study, a new type of hydrogen peroxide (H2O[Formula: see text]-activated photoacoustic nanoprobe [Mn-AH nanoscale coordinat...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2022-09-01
|
| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545822500262 |
| _version_ | 1798019563367956480 |
|---|---|
| author | Jiayue Zhao Fei Gong Nailin Yang Huali Lei Zhihui Han Yuqi Yang Liang Cheng |
| author_facet | Jiayue Zhao Fei Gong Nailin Yang Huali Lei Zhihui Han Yuqi Yang Liang Cheng |
| author_sort | Jiayue Zhao |
| collection | DOAJ |
| description | Photoacoustic (PA) imaging with much deeper tissue penetration and better spatial resolution had been widely employed for the prevention and diagnosis of many diseases. In this study, a new type of hydrogen peroxide (H2O[Formula: see text]-activated photoacoustic nanoprobe [Mn-AH nanoscale coordination polymer nanodots (NCPs)] was successfully synthesized by a simple one-step method in water phase containing 2,2[Formula: see text]-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), horse radish peroxidase (HRP), and manganese ion (Mn[Formula: see text]. After modification by polyethylene glycol (PEG), Mn-AH NCPs exhibited excellent stability and biocompatibility for in vivo H2O2-responsive chromogenic assay with great specificity and sensitivity. In the presence of H2O2, colorless ABTS would be converted by HRP into the oxidized form with strong near-infrared (NIR) absorbance, enabling photoacoustic detection of endogenous H2O2. Using H2O2-activated Mn-AH NCPs, we have successfully performed PA imaging and H2O2 detection of subcutaneous murine colon CT26 tumor and deep-seated orthotopic bladder tumor. Due to the inherent Mn element existence inside the Mn-AH, this nanoprobe also serves as a good T1-weighted magnetic resonance imaging (MRI) contrast agent simultaneously. Lastly, after accomplishing its imaging functions, the Mn-AH NCPs could be cleared out from the body without any long-term toxicity, providing a new opportunity for cancer diagnosis and treatment. |
| first_indexed | 2024-04-11T16:42:36Z |
| format | Article |
| id | doaj.art-6d96737cec8c4aad82d5645546d55d08 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-04-11T16:42:36Z |
| publishDate | 2022-09-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-6d96737cec8c4aad82d5645546d55d082022-12-22T04:13:38ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052022-09-01150510.1142/S1793545822500262H2O2-sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assayJiayue Zhao0Fei Gong1Nailin Yang2Huali Lei3Zhihui Han4Yuqi Yang5Liang Cheng6Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123 Jiangsu, P. R. ChinaJiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123 Jiangsu, P. R. ChinaJiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123 Jiangsu, P. R. ChinaJiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123 Jiangsu, P. R. ChinaJiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123 Jiangsu, P. R. ChinaJiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123 Jiangsu, P. R. ChinaJiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123 Jiangsu, P. R. ChinaPhotoacoustic (PA) imaging with much deeper tissue penetration and better spatial resolution had been widely employed for the prevention and diagnosis of many diseases. In this study, a new type of hydrogen peroxide (H2O[Formula: see text]-activated photoacoustic nanoprobe [Mn-AH nanoscale coordination polymer nanodots (NCPs)] was successfully synthesized by a simple one-step method in water phase containing 2,2[Formula: see text]-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), horse radish peroxidase (HRP), and manganese ion (Mn[Formula: see text]. After modification by polyethylene glycol (PEG), Mn-AH NCPs exhibited excellent stability and biocompatibility for in vivo H2O2-responsive chromogenic assay with great specificity and sensitivity. In the presence of H2O2, colorless ABTS would be converted by HRP into the oxidized form with strong near-infrared (NIR) absorbance, enabling photoacoustic detection of endogenous H2O2. Using H2O2-activated Mn-AH NCPs, we have successfully performed PA imaging and H2O2 detection of subcutaneous murine colon CT26 tumor and deep-seated orthotopic bladder tumor. Due to the inherent Mn element existence inside the Mn-AH, this nanoprobe also serves as a good T1-weighted magnetic resonance imaging (MRI) contrast agent simultaneously. Lastly, after accomplishing its imaging functions, the Mn-AH NCPs could be cleared out from the body without any long-term toxicity, providing a new opportunity for cancer diagnosis and treatment.https://www.worldscientific.com/doi/10.1142/S1793545822500262Nanoscale coordination polymers (NCPs)PA imagingMRIH2O2 detectionmetabolic nanoprobes |
| spellingShingle | Jiayue Zhao Fei Gong Nailin Yang Huali Lei Zhihui Han Yuqi Yang Liang Cheng H2O2-sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assay Journal of Innovative Optical Health Sciences Nanoscale coordination polymers (NCPs) PA imaging MRI H2O2 detection metabolic nanoprobes |
| title | H2O2-sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assay |
| title_full | H2O2-sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assay |
| title_fullStr | H2O2-sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assay |
| title_full_unstemmed | H2O2-sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assay |
| title_short | H2O2-sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assay |
| title_sort | h2o2 sensitive nanoscale coordination polymers for photoacoustic tumors imaging via in vivo chromogenic assay |
| topic | Nanoscale coordination polymers (NCPs) PA imaging MRI H2O2 detection metabolic nanoprobes |
| url | https://www.worldscientific.com/doi/10.1142/S1793545822500262 |
| work_keys_str_mv | AT jiayuezhao h2o2sensitivenanoscalecoordinationpolymersforphotoacoustictumorsimagingviainvivochromogenicassay AT feigong h2o2sensitivenanoscalecoordinationpolymersforphotoacoustictumorsimagingviainvivochromogenicassay AT nailinyang h2o2sensitivenanoscalecoordinationpolymersforphotoacoustictumorsimagingviainvivochromogenicassay AT hualilei h2o2sensitivenanoscalecoordinationpolymersforphotoacoustictumorsimagingviainvivochromogenicassay AT zhihuihan h2o2sensitivenanoscalecoordinationpolymersforphotoacoustictumorsimagingviainvivochromogenicassay AT yuqiyang h2o2sensitivenanoscalecoordinationpolymersforphotoacoustictumorsimagingviainvivochromogenicassay AT liangcheng h2o2sensitivenanoscalecoordinationpolymersforphotoacoustictumorsimagingviainvivochromogenicassay |