Deciphering Differential Life Stage Radioinduced Reproductive Decline in <i>Caenorhabditis elegans</i> through Lipid Analysis
Wildlife is chronically exposed to various sources of ionizing radiations, both environmental or anthropic, due to nuclear energy use, which can induce several defects in organisms. In invertebrates, reproduction, which directly impacts population dynamics, has been found to be the most radiosensiti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/19/10277 |
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| author | Elizabeth Dufourcq-Sekatcheff Stephan Cuiné Yonghua Li-Beisson Loïc Quevarec Myriam Richaud Simon Galas Sandrine Frelon |
| author_facet | Elizabeth Dufourcq-Sekatcheff Stephan Cuiné Yonghua Li-Beisson Loïc Quevarec Myriam Richaud Simon Galas Sandrine Frelon |
| author_sort | Elizabeth Dufourcq-Sekatcheff |
| collection | DOAJ |
| description | Wildlife is chronically exposed to various sources of ionizing radiations, both environmental or anthropic, due to nuclear energy use, which can induce several defects in organisms. In invertebrates, reproduction, which directly impacts population dynamics, has been found to be the most radiosensitive endpoint. Understanding the underlying molecular pathways inducing this reproduction decrease can help in predicting the effects at larger scales (i.e., population). In this study, we used a life stage dependent approach in order to better understand the molecular determinants of reproduction decrease in the roundworm <i>C. elegans</i>. Worms were chronically exposed to 50 mGy·h<sup>−1</sup> external gamma ionizing radiations throughout different developmental periods (namely embryogenesis, gametogenesis, and full development). Then, in addition to reproduction parameters, we performed a wide analysis of lipids (different class and fatty acid via FAMES), which are both important signaling molecules for reproduction and molecular targets of oxidative stress. Our results showed that reproductive defects are life stage dependent, that lipids are differently misregulated according to the considered exposure (e.g., upon embryogenesis and full development) and do not fully explain radiation induced reproductive defects. Finally, our results enable us to propose a conceptual model of lipid signaling after radiation stress in which both the soma and the germline participate. |
| first_indexed | 2024-03-10T07:00:47Z |
| format | Article |
| id | doaj.art-6d97b3f4aef2444bb9dd5a34d7c8e29d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T07:00:47Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6d97b3f4aef2444bb9dd5a34d7c8e29d2023-11-22T16:07:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-0122191027710.3390/ijms221910277Deciphering Differential Life Stage Radioinduced Reproductive Decline in <i>Caenorhabditis elegans</i> through Lipid AnalysisElizabeth Dufourcq-Sekatcheff0Stephan Cuiné1Yonghua Li-Beisson2Loïc Quevarec3Myriam Richaud4Simon Galas5Sandrine Frelon6IRSN PSE-ENV Laboratoire de Recherche sur les Effets des Radionucléides sur L’environnement, Cadarache, 13115 Saint Paul-Lez-Durance, FranceInstitut de Biosciences et Biotechnologies Aix-Marseille, Aix Marseille University, CEA, CNRS, BIAM, CEA Cadarache, 13108 Saint Paul-Lez-Durance, FranceInstitut de Biosciences et Biotechnologies Aix-Marseille, Aix Marseille University, CEA, CNRS, BIAM, CEA Cadarache, 13108 Saint Paul-Lez-Durance, FranceIRSN PSE-ENV Laboratoire de Recherche sur les Effets des Radionucléides sur L’environnement, Cadarache, 13115 Saint Paul-Lez-Durance, FranceCNRS, ENSCM, IBMM Université de Montpellier, 34093 Montpellier, FranceCNRS, ENSCM, IBMM Université de Montpellier, 34093 Montpellier, FranceIRSN PSE-ENV Laboratoire de Recherche sur les Effets des Radionucléides sur L’environnement, Cadarache, 13115 Saint Paul-Lez-Durance, FranceWildlife is chronically exposed to various sources of ionizing radiations, both environmental or anthropic, due to nuclear energy use, which can induce several defects in organisms. In invertebrates, reproduction, which directly impacts population dynamics, has been found to be the most radiosensitive endpoint. Understanding the underlying molecular pathways inducing this reproduction decrease can help in predicting the effects at larger scales (i.e., population). In this study, we used a life stage dependent approach in order to better understand the molecular determinants of reproduction decrease in the roundworm <i>C. elegans</i>. Worms were chronically exposed to 50 mGy·h<sup>−1</sup> external gamma ionizing radiations throughout different developmental periods (namely embryogenesis, gametogenesis, and full development). Then, in addition to reproduction parameters, we performed a wide analysis of lipids (different class and fatty acid via FAMES), which are both important signaling molecules for reproduction and molecular targets of oxidative stress. Our results showed that reproductive defects are life stage dependent, that lipids are differently misregulated according to the considered exposure (e.g., upon embryogenesis and full development) and do not fully explain radiation induced reproductive defects. Finally, our results enable us to propose a conceptual model of lipid signaling after radiation stress in which both the soma and the germline participate.https://www.mdpi.com/1422-0067/22/19/10277ionizing radiationoxidative stresslipidsreproduction<i>Caenorhabditis elegans</i>chronic exposure |
| spellingShingle | Elizabeth Dufourcq-Sekatcheff Stephan Cuiné Yonghua Li-Beisson Loïc Quevarec Myriam Richaud Simon Galas Sandrine Frelon Deciphering Differential Life Stage Radioinduced Reproductive Decline in <i>Caenorhabditis elegans</i> through Lipid Analysis International Journal of Molecular Sciences ionizing radiation oxidative stress lipids reproduction <i>Caenorhabditis elegans</i> chronic exposure |
| title | Deciphering Differential Life Stage Radioinduced Reproductive Decline in <i>Caenorhabditis elegans</i> through Lipid Analysis |
| title_full | Deciphering Differential Life Stage Radioinduced Reproductive Decline in <i>Caenorhabditis elegans</i> through Lipid Analysis |
| title_fullStr | Deciphering Differential Life Stage Radioinduced Reproductive Decline in <i>Caenorhabditis elegans</i> through Lipid Analysis |
| title_full_unstemmed | Deciphering Differential Life Stage Radioinduced Reproductive Decline in <i>Caenorhabditis elegans</i> through Lipid Analysis |
| title_short | Deciphering Differential Life Stage Radioinduced Reproductive Decline in <i>Caenorhabditis elegans</i> through Lipid Analysis |
| title_sort | deciphering differential life stage radioinduced reproductive decline in i caenorhabditis elegans i through lipid analysis |
| topic | ionizing radiation oxidative stress lipids reproduction <i>Caenorhabditis elegans</i> chronic exposure |
| url | https://www.mdpi.com/1422-0067/22/19/10277 |
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