Molecular characterization of the lipL41 gene of Leptospira interrogans vaccinal serovars in Iran
Leptospirosis caused by infection with pathogenic leptospires, which is the most prevalent zoonotic disease in the world. The outer membrane proteins (OMPs) of pathogenic leptospires such as LipL41 play a crucial role in pathogenesis of this disease. Therefore a major challenge to develop an effec...
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Razi Vaccine and Serum Research Institute
2015-10-01
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| Series: | Archives of Razi Institute |
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| Online Access: | http://www.archrazi.com/browse.php?a_id=575&sid=1&slc_lang=en |
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| author | M.S. Soltani P. Khaki S. Moradi bidhendi M.H. Shahhosseiny |
| author_facet | M.S. Soltani P. Khaki S. Moradi bidhendi M.H. Shahhosseiny |
| author_sort | M.S. Soltani |
| collection | DOAJ |
| description | Leptospirosis caused by infection with pathogenic leptospires, which is the most prevalent zoonotic disease in
the world. The outer membrane proteins (OMPs) of pathogenic leptospires such as LipL41 play a crucial role
in pathogenesis of this disease. Therefore a major challenge to develop an effective vaccine against
leptospirosis is application of basic research on the OMPs of leptospires to improve vaccine development. The
aim of this study was cloning and analyzing of the lipL41 gene from vaccinal serovars of leptospires in Iran, in
order to identify genetic conservation of this gene. Three vaccinal serovars of Leptospira were used in this
study. The lipL41 gene of these serovars were amplified and cloned in the pTZ57R/T vector. The recombinant
clones were confirmed by colony-PCR and sequencing. The sequenced genes were analyzed for their
homology between them and other submitted sequences in Genbank database using the BLAST and
MegAlign program. PCR amplification of the lipL41 gene resulted in the 1065 bp gene product in vaccinal
serovars tested. In our study, nucleotide sequencing results showed high similarity (>94%) within the
leptospiral vaccinal serovars. The genetic conservation of the lipL41gene among different serovars of Leptospira
indicated the capacity of utilization of this gene for development of recombinant vaccine against leptospirosis. |
| first_indexed | 2024-12-11T12:00:45Z |
| format | Article |
| id | doaj.art-6d9845e6180e4344939990d61ecf1fa3 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-11T12:00:45Z |
| publishDate | 2015-10-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-6d9845e6180e4344939990d61ecf1fa32022-12-22T01:08:05ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722015-10-017003145150Molecular characterization of the lipL41 gene of Leptospira interrogans vaccinal serovars in IranM.S. Soltani0P. Khaki1S. Moradi bidhendi 2M.H. Shahhosseiny3Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranLeptospira Reference Laboratory, Department of Microbiology, Razi Vaccine & Serum Research Institute, Karaj, IranLeptospira Reference Laboratory, Department of Microbiology, Razi Vaccine & Serum Research Institute, Karaj, IranDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranLeptospirosis caused by infection with pathogenic leptospires, which is the most prevalent zoonotic disease in the world. The outer membrane proteins (OMPs) of pathogenic leptospires such as LipL41 play a crucial role in pathogenesis of this disease. Therefore a major challenge to develop an effective vaccine against leptospirosis is application of basic research on the OMPs of leptospires to improve vaccine development. The aim of this study was cloning and analyzing of the lipL41 gene from vaccinal serovars of leptospires in Iran, in order to identify genetic conservation of this gene. Three vaccinal serovars of Leptospira were used in this study. The lipL41 gene of these serovars were amplified and cloned in the pTZ57R/T vector. The recombinant clones were confirmed by colony-PCR and sequencing. The sequenced genes were analyzed for their homology between them and other submitted sequences in Genbank database using the BLAST and MegAlign program. PCR amplification of the lipL41 gene resulted in the 1065 bp gene product in vaccinal serovars tested. In our study, nucleotide sequencing results showed high similarity (>94%) within the leptospiral vaccinal serovars. The genetic conservation of the lipL41gene among different serovars of Leptospira indicated the capacity of utilization of this gene for development of recombinant vaccine against leptospirosis.http://www.archrazi.com/browse.php?a_id=575&sid=1&slc_lang=enLeptospirosislipL41 geneMolecular characterizationSequencingVaccinal serovars of LeptospiVaccinal serovars of Leptospira |
| spellingShingle | M.S. Soltani P. Khaki S. Moradi bidhendi M.H. Shahhosseiny Molecular characterization of the lipL41 gene of Leptospira interrogans vaccinal serovars in Iran Archives of Razi Institute Leptospirosis lipL41 gene Molecular characterization Sequencing Vaccinal serovars of Leptospi Vaccinal serovars of Leptospira |
| title | Molecular characterization of the lipL41 gene of Leptospira interrogans vaccinal serovars in Iran |
| title_full | Molecular characterization of the lipL41 gene of Leptospira interrogans vaccinal serovars in Iran |
| title_fullStr | Molecular characterization of the lipL41 gene of Leptospira interrogans vaccinal serovars in Iran |
| title_full_unstemmed | Molecular characterization of the lipL41 gene of Leptospira interrogans vaccinal serovars in Iran |
| title_short | Molecular characterization of the lipL41 gene of Leptospira interrogans vaccinal serovars in Iran |
| title_sort | molecular characterization of the lipl41 gene of leptospira interrogans vaccinal serovars in iran |
| topic | Leptospirosis lipL41 gene Molecular characterization Sequencing Vaccinal serovars of Leptospi Vaccinal serovars of Leptospira |
| url | http://www.archrazi.com/browse.php?a_id=575&sid=1&slc_lang=en |
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