Comparative Evaluation of Three Herbal Solutions on the Disinfection of Guttapercha Cones: An In vitro Study
Introduction: Guttapercha (GP) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. Supplementary decontamination of GP cones is critical. As...
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| Format: | Article |
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JCDR Research and Publications Private Limited
2017-03-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/9487/25208_CE[Ra1]_F(GH)_PF1(PrG_RK)_PFA(GH)_PF2(NE_SY_DK).pdf |
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| author | Pratap Kumar Mukka Samba Shiva Rao Pola Nanda Kumar Kommineni Mayuri Sailaja Pachalla Anand Ramtheerth Karne Karthik Labishetty Supriya Kommineni Praveen Chilakabathini |
| author_facet | Pratap Kumar Mukka Samba Shiva Rao Pola Nanda Kumar Kommineni Mayuri Sailaja Pachalla Anand Ramtheerth Karne Karthik Labishetty Supriya Kommineni Praveen Chilakabathini |
| author_sort | Pratap Kumar Mukka |
| collection | DOAJ |
| description | Introduction: Guttapercha (GP) cones are usually supplied in
aseptic, sealed packages from the manufacturers, but once
exposed to the dental office environment or even by handling;
they can be contaminated by a number of microorganisms.
Supplementary decontamination of GP cones is critical. As
they cannot be sterilized by moist or dry heat, cold sterilization,
using disinfectants should be used.
Aim: The aim of the study was to evaluate rapid, reliable,
convenient and effective method of disinfection of GP cones
using readily available herbal solutions such as Aloevera Juice,
Amla Juice and Pancha Tulsi.
Materials and Methods: Ninety GP cones were selected for
the study. Based on the contaminants used, 80 GP cones
were divided into two groups: Group A and Group B with 40
cones in each group and the remaining 10 cones which were
uncontaminated served as negative control. The cones from
Group A were contaminated with Staphylococcus aureus (S.
aureus) and Group B were contaminated with Enterococcus
faecalis (E. faecalis). The contaminated cones from both groups
were subdivided into four groups with 10 cones in each group.
Group I cones were disinfected with Aloevera Juice, Group II
with Amla Juice and Group III with Pancha Tulsi. The fourth
group consisted of GP cones without any disinfection which
served as positive control. All the cones were then incubated
in thioglycollate media for seven days. The thioglycollate media
was subcultured and colony forming units were counted. The
data were analyzed by one way ANOVA (analysis of variance)
and Post-hoc Tukey test using SPSS 17.0 software.
Results: Mean colony forming units were compared in all
the groups and there was a statistically significant difference
present among the groups (p<0.01). Pancha Tulsi was found to
be most effective disinfectant followed by Amla Juice.
Conclusion: All the herbal solutions were found to be effective in
the disinfection of GP points. However, Pancha Tulsi possesses
superior antibacterial activity when compared with Aloevera
Juice and Amla juice. |
| first_indexed | 2024-12-10T23:40:04Z |
| format | Article |
| id | doaj.art-6d99aead0b7b4bba90e9d551771e8e4e |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-10T23:40:04Z |
| publishDate | 2017-03-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-6d99aead0b7b4bba90e9d551771e8e4e2022-12-22T01:29:03ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-03-01113ZC21ZC2410.7860/JCDR/2017/25208.9487Comparative Evaluation of Three Herbal Solutions on the Disinfection of Guttapercha Cones: An In vitro StudyPratap Kumar Mukka0Samba Shiva Rao Pola1Nanda Kumar Kommineni2Mayuri Sailaja Pachalla3Anand Ramtheerth Karne4Karthik Labishetty5Supriya Kommineni6Praveen Chilakabathini7Professor, Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.Professor, Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.Professor and Head, Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.Postgraduate Student, Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.Senior Lecturer, Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.Senior Lecturer, Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.Postgraduate Student, Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.Postgraduate Student, Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.Introduction: Guttapercha (GP) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. Supplementary decontamination of GP cones is critical. As they cannot be sterilized by moist or dry heat, cold sterilization, using disinfectants should be used. Aim: The aim of the study was to evaluate rapid, reliable, convenient and effective method of disinfection of GP cones using readily available herbal solutions such as Aloevera Juice, Amla Juice and Pancha Tulsi. Materials and Methods: Ninety GP cones were selected for the study. Based on the contaminants used, 80 GP cones were divided into two groups: Group A and Group B with 40 cones in each group and the remaining 10 cones which were uncontaminated served as negative control. The cones from Group A were contaminated with Staphylococcus aureus (S. aureus) and Group B were contaminated with Enterococcus faecalis (E. faecalis). The contaminated cones from both groups were subdivided into four groups with 10 cones in each group. Group I cones were disinfected with Aloevera Juice, Group II with Amla Juice and Group III with Pancha Tulsi. The fourth group consisted of GP cones without any disinfection which served as positive control. All the cones were then incubated in thioglycollate media for seven days. The thioglycollate media was subcultured and colony forming units were counted. The data were analyzed by one way ANOVA (analysis of variance) and Post-hoc Tukey test using SPSS 17.0 software. Results: Mean colony forming units were compared in all the groups and there was a statistically significant difference present among the groups (p<0.01). Pancha Tulsi was found to be most effective disinfectant followed by Amla Juice. Conclusion: All the herbal solutions were found to be effective in the disinfection of GP points. However, Pancha Tulsi possesses superior antibacterial activity when compared with Aloevera Juice and Amla juice.https://jcdr.net/articles/PDF/9487/25208_CE[Ra1]_F(GH)_PF1(PrG_RK)_PFA(GH)_PF2(NE_SY_DK).pdfaloevera juiceamla juicedigital colony counterpancha tulsi |
| spellingShingle | Pratap Kumar Mukka Samba Shiva Rao Pola Nanda Kumar Kommineni Mayuri Sailaja Pachalla Anand Ramtheerth Karne Karthik Labishetty Supriya Kommineni Praveen Chilakabathini Comparative Evaluation of Three Herbal Solutions on the Disinfection of Guttapercha Cones: An In vitro Study Journal of Clinical and Diagnostic Research aloevera juice amla juice digital colony counter pancha tulsi |
| title | Comparative Evaluation of Three Herbal Solutions on the Disinfection of Guttapercha Cones: An In vitro Study |
| title_full | Comparative Evaluation of Three Herbal Solutions on the Disinfection of Guttapercha Cones: An In vitro Study |
| title_fullStr | Comparative Evaluation of Three Herbal Solutions on the Disinfection of Guttapercha Cones: An In vitro Study |
| title_full_unstemmed | Comparative Evaluation of Three Herbal Solutions on the Disinfection of Guttapercha Cones: An In vitro Study |
| title_short | Comparative Evaluation of Three Herbal Solutions on the Disinfection of Guttapercha Cones: An In vitro Study |
| title_sort | comparative evaluation of three herbal solutions on the disinfection of guttapercha cones an in vitro study |
| topic | aloevera juice amla juice digital colony counter pancha tulsi |
| url | https://jcdr.net/articles/PDF/9487/25208_CE[Ra1]_F(GH)_PF1(PrG_RK)_PFA(GH)_PF2(NE_SY_DK).pdf |
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