Ultrastructural characterization and quantification of hemocytes in engorged female Amblyomma sculptum ticks
Amblyomma sculptum (formerly Amblyomma cajennense) ticks have been implicated in the transmission of pathogens that cause diseases in animals and humans. Their wide geographic distribution and high impact on animal health and zoonotic disease transmission highlight the importance of studying and imp...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Ticks and Tick-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X24000050 |
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| author | Andressa Aparecida de Lima Reis Barbara Rauta de Avelar Marisa Beatriz da Silva Rocha Debora Azevedo Borges Diefrey Ribeiro Campos Jessica Fiorotti Patrícia Silva Golo Fabio Barbour Scott |
| author_facet | Andressa Aparecida de Lima Reis Barbara Rauta de Avelar Marisa Beatriz da Silva Rocha Debora Azevedo Borges Diefrey Ribeiro Campos Jessica Fiorotti Patrícia Silva Golo Fabio Barbour Scott |
| author_sort | Andressa Aparecida de Lima Reis |
| collection | DOAJ |
| description | Amblyomma sculptum (formerly Amblyomma cajennense) ticks have been implicated in the transmission of pathogens that cause diseases in animals and humans. Their wide geographic distribution and high impact on animal health and zoonotic disease transmission highlight the importance of studying and implementing effective control measures to mitigate the risks associated with this tick species. The aim of this study was to quantify and characterize the morphology and the ultrastructure of different types of hemocytes in the hemolymph in engorged A. sculptum females fed on rabbits. The hemolymph samples were collected by perforation of the cuticle in the dorsal region. Hemocyte types, sizes, and differential counts were determined using light microscopy, while ultrastructural analysis of hemocytes was performed using transmission electron microscopy. The average number of total hemocytes in the hemolymph was 1024 ± 597.6 cells µL−1. Five morphologically distinct cell types were identified in A. sculptum females: prohemocytes (6 % ± 8.8), plasmatocytes (10 % ± 7.7), granulocytes (78 % ± 12.2), spherulocytes (5 % ± 4.48), and oenocytoids (1 % ± 1.6). In general, prohemocytes were the smallest hemocytes. The ultrastructural morphology of A. sculptum hemocytes described in the present study agrees with the findings for other hard ticks. This is the first study to investigate ultrastructural characteristics of hemocytes of female A. sculptum ticks. |
| first_indexed | 2024-03-08T10:30:29Z |
| format | Article |
| id | doaj.art-6d9b90e3789741cdba165cb05617b3c4 |
| institution | Directory Open Access Journal |
| issn | 1877-9603 |
| language | English |
| last_indexed | 2024-04-24T20:25:54Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj.art-6d9b90e3789741cdba165cb05617b3c42024-03-22T05:39:03ZengElsevierTicks and Tick-Borne Diseases1877-96032024-05-01153102312Ultrastructural characterization and quantification of hemocytes in engorged female Amblyomma sculptum ticksAndressa Aparecida de Lima Reis0Barbara Rauta de Avelar1Marisa Beatriz da Silva Rocha2Debora Azevedo Borges3Diefrey Ribeiro Campos4Jessica Fiorotti5Patrícia Silva Golo6Fabio Barbour Scott7Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Km 07, Zona Rural, BR-465, Seropédica, RJ 23890-000, Brazil; Corresponding author.Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Km 07, Zona Rural, BR-465, Seropédica, RJ 23890-000, BrazilDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Km 07, Zona Rural, BR-465, Seropédica, RJ 23890-000, BrazilDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Km 07, Zona Rural, BR-465, Seropédica, RJ 23890-000, BrazilDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Km 07, Zona Rural, BR-465, Seropédica, RJ 23890-000, BrazilDepartment of Biochemistry and Immunology, Ribeirão Preto School of Medicine, University of São Paulo, São Paulo 14040-900, BrazilDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Km 07, Zona Rural, BR-465, Seropédica, RJ 23890-000, BrazilDepartment of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Km 07, Zona Rural, BR-465, Seropédica, RJ 23890-000, BrazilAmblyomma sculptum (formerly Amblyomma cajennense) ticks have been implicated in the transmission of pathogens that cause diseases in animals and humans. Their wide geographic distribution and high impact on animal health and zoonotic disease transmission highlight the importance of studying and implementing effective control measures to mitigate the risks associated with this tick species. The aim of this study was to quantify and characterize the morphology and the ultrastructure of different types of hemocytes in the hemolymph in engorged A. sculptum females fed on rabbits. The hemolymph samples were collected by perforation of the cuticle in the dorsal region. Hemocyte types, sizes, and differential counts were determined using light microscopy, while ultrastructural analysis of hemocytes was performed using transmission electron microscopy. The average number of total hemocytes in the hemolymph was 1024 ± 597.6 cells µL−1. Five morphologically distinct cell types were identified in A. sculptum females: prohemocytes (6 % ± 8.8), plasmatocytes (10 % ± 7.7), granulocytes (78 % ± 12.2), spherulocytes (5 % ± 4.48), and oenocytoids (1 % ± 1.6). In general, prohemocytes were the smallest hemocytes. The ultrastructural morphology of A. sculptum hemocytes described in the present study agrees with the findings for other hard ticks. This is the first study to investigate ultrastructural characteristics of hemocytes of female A. sculptum ticks.http://www.sciencedirect.com/science/article/pii/S1877959X24000050HemocytesTick cell quantificationTick immunityMorphologyUltrastructureTransmission electron microscopy |
| spellingShingle | Andressa Aparecida de Lima Reis Barbara Rauta de Avelar Marisa Beatriz da Silva Rocha Debora Azevedo Borges Diefrey Ribeiro Campos Jessica Fiorotti Patrícia Silva Golo Fabio Barbour Scott Ultrastructural characterization and quantification of hemocytes in engorged female Amblyomma sculptum ticks Ticks and Tick-Borne Diseases Hemocytes Tick cell quantification Tick immunity Morphology Ultrastructure Transmission electron microscopy |
| title | Ultrastructural characterization and quantification of hemocytes in engorged female Amblyomma sculptum ticks |
| title_full | Ultrastructural characterization and quantification of hemocytes in engorged female Amblyomma sculptum ticks |
| title_fullStr | Ultrastructural characterization and quantification of hemocytes in engorged female Amblyomma sculptum ticks |
| title_full_unstemmed | Ultrastructural characterization and quantification of hemocytes in engorged female Amblyomma sculptum ticks |
| title_short | Ultrastructural characterization and quantification of hemocytes in engorged female Amblyomma sculptum ticks |
| title_sort | ultrastructural characterization and quantification of hemocytes in engorged female amblyomma sculptum ticks |
| topic | Hemocytes Tick cell quantification Tick immunity Morphology Ultrastructure Transmission electron microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X24000050 |
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