Phytochemical characterization, antioxidant potential and antimicrobial activity of Averrhoa carambola L. (Oxalidaceae) against multiresistant pathogens
Abstract The objective of this work was to perform the phytochemical characterization, to determine total phenols, antioxidant (AAO%) and antimicrobial potential of the ethanolic extracts of carambola. The phytochemical study was carried out through a qualitative analysis of the chemical constituent...
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| Language: | English |
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Instituto Internacional de Ecologia
2020-08-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842021000300509&tlng=en |
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| author | K. B. Silva C. T. S. Pinheiro C. R. M. Soares M. A. Souza T. J. Matos-Rocha S. A. Fonseca J. M. S. J. Pavão J. G. Costa L. L. S. Pires A. F. Santos |
| author_facet | K. B. Silva C. T. S. Pinheiro C. R. M. Soares M. A. Souza T. J. Matos-Rocha S. A. Fonseca J. M. S. J. Pavão J. G. Costa L. L. S. Pires A. F. Santos |
| author_sort | K. B. Silva |
| collection | DOAJ |
| description | Abstract The objective of this work was to perform the phytochemical characterization, to determine total phenols, antioxidant (AAO%) and antimicrobial potential of the ethanolic extracts of carambola. The phytochemical study was carried out through a qualitative analysis of the chemical constituents and quantitative determination of the phenol content By the Folin-Ciocalteu test. Qualitative and quantitative antioxidant tests were performed using the DPPH method (2,2 diphenyl-1-picryl-hydrazila) and iron reduction (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The presence of pyrogallic tannins, steroids and saponins has been identified. The highest total phenol content, quantified in the samples, was found in the stem bark (0.0866 mgEAG/g) and in the fruit (0.0734 mgEAG/g). In the antioxidant evaluation, the extracts of the green fruit bagasse (AAO% 71.9%,) and stem bark at 50 μg/mL (AAO% 94%) with CE50 23.7 μg/mL. Leaf extracts, stem bark, ripe fruit bagasse and green fruit bagasse presented MICs of 100 μg/mL against multiresistant pathogenic bacteria and fungi. |
| first_indexed | 2024-12-18T02:49:47Z |
| format | Article |
| id | doaj.art-6d9cebb9cbb54449aaa0d46859cbea74 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-18T02:49:47Z |
| publishDate | 2020-08-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-6d9cebb9cbb54449aaa0d46859cbea742022-12-21T21:23:29ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752020-08-0181350951510.1590/1519-6984.220259Phytochemical characterization, antioxidant potential and antimicrobial activity of Averrhoa carambola L. (Oxalidaceae) against multiresistant pathogensK. B. SilvaC. T. S. PinheiroC. R. M. SoaresM. A. Souzahttps://orcid.org/0000-0003-0823-1957T. J. Matos-Rochahttps://orcid.org/0000-0001-5153-6583S. A. Fonsecahttps://orcid.org/0000-0003-2091-7951J. M. S. J. Pavãohttps://orcid.org/0000-0002-5217-3857J. G. Costahttps://orcid.org/0000-0002-0761-0755L. L. S. Pireshttps://orcid.org/0000-0003-1230-4549A. F. Santoshttps://orcid.org/0000-0001-6049-9446Abstract The objective of this work was to perform the phytochemical characterization, to determine total phenols, antioxidant (AAO%) and antimicrobial potential of the ethanolic extracts of carambola. The phytochemical study was carried out through a qualitative analysis of the chemical constituents and quantitative determination of the phenol content By the Folin-Ciocalteu test. Qualitative and quantitative antioxidant tests were performed using the DPPH method (2,2 diphenyl-1-picryl-hydrazila) and iron reduction (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The presence of pyrogallic tannins, steroids and saponins has been identified. The highest total phenol content, quantified in the samples, was found in the stem bark (0.0866 mgEAG/g) and in the fruit (0.0734 mgEAG/g). In the antioxidant evaluation, the extracts of the green fruit bagasse (AAO% 71.9%,) and stem bark at 50 μg/mL (AAO% 94%) with CE50 23.7 μg/mL. Leaf extracts, stem bark, ripe fruit bagasse and green fruit bagasse presented MICs of 100 μg/mL against multiresistant pathogenic bacteria and fungi.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842021000300509&tlng=enphytochemical screeningtotal phenolsphenolic compoundscarambola |
| spellingShingle | K. B. Silva C. T. S. Pinheiro C. R. M. Soares M. A. Souza T. J. Matos-Rocha S. A. Fonseca J. M. S. J. Pavão J. G. Costa L. L. S. Pires A. F. Santos Phytochemical characterization, antioxidant potential and antimicrobial activity of Averrhoa carambola L. (Oxalidaceae) against multiresistant pathogens Brazilian Journal of Biology phytochemical screening total phenols phenolic compounds carambola |
| title | Phytochemical characterization, antioxidant potential and antimicrobial activity of Averrhoa carambola L. (Oxalidaceae) against multiresistant pathogens |
| title_full | Phytochemical characterization, antioxidant potential and antimicrobial activity of Averrhoa carambola L. (Oxalidaceae) against multiresistant pathogens |
| title_fullStr | Phytochemical characterization, antioxidant potential and antimicrobial activity of Averrhoa carambola L. (Oxalidaceae) against multiresistant pathogens |
| title_full_unstemmed | Phytochemical characterization, antioxidant potential and antimicrobial activity of Averrhoa carambola L. (Oxalidaceae) against multiresistant pathogens |
| title_short | Phytochemical characterization, antioxidant potential and antimicrobial activity of Averrhoa carambola L. (Oxalidaceae) against multiresistant pathogens |
| title_sort | phytochemical characterization antioxidant potential and antimicrobial activity of averrhoa carambola l oxalidaceae against multiresistant pathogens |
| topic | phytochemical screening total phenols phenolic compounds carambola |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842021000300509&tlng=en |
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