Eugenyl-2-Hydroxypropyl Methacrylate-Incorporated Experimental Dental Composite: Degree of Polymerization and In Vitro Cytotoxicity Evaluation
The aim of this study was to evaluate the properties of new dental formulations containing eugenyl-2-hydroxypropyl methacrylate (EgGMA) monomer, as restorative dental material, in terms of their degree of photopolymerization and cytotoxicity. The target model composites (TBEg0, TBEg2.5, and TBEg5) w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/2/277 |
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| author | Abdel-Basit Al-Odayni Dalal H. Alotaibi Waseem Sharaf Saeed Abdullah Al-Kahtani Ali Assiri Fahad M. Alkhtani Ali Alrahlah |
| author_facet | Abdel-Basit Al-Odayni Dalal H. Alotaibi Waseem Sharaf Saeed Abdullah Al-Kahtani Ali Assiri Fahad M. Alkhtani Ali Alrahlah |
| author_sort | Abdel-Basit Al-Odayni |
| collection | DOAJ |
| description | The aim of this study was to evaluate the properties of new dental formulations containing eugenyl-2-hydroxypropyl methacrylate (EgGMA) monomer, as restorative dental material, in terms of their degree of photopolymerization and cytotoxicity. The target model composites (TBEg0, TBEg2.5, and TBEg5) were prepared by mixing 35% organic matrix (TEGDMA/BisGMA (50/50 wt%) of which 0, 2.5, and 5 wt%, respectively, were replaced with EgGMA monomer) with 65% filler (silanized hydroxyapatite (HA)/zinc oxide (ZnO<sub>2</sub>), 4:3 by weight). The vinylic double-bond conversion (DC) after light-curing was studied using Fourier transform infrared technique whereas cell viability was in vitro tested using primary human gingival fibroblasts cells over 7 days by means of AlamarBlue colorimetric assay. The obtained data were statistically analyzed using ANOVA and Tukey post-hoc tests. The results revealed no significant difference in DC between TBEg2.5 (66.49%) and control (TBEg0; 68.74%), whereas both differ significantly with TBEg5, likely due to the inhibitory effect of eugenol moiety at high concentration. The cell viability test indicated that all the composites are biocompatible. No significant difference was counted between TBEg2.5 and TBEg5, however, both differed significantly from the control (TBEg0). Thus, even though its apparent negative effect on polymerization, EgGMA is potentially safer than bisphenol-derived monomers. Such potential properties may encourage further investigations on term of EgGMA amount optimization, compatibility with other dental resins, and antimicrobial activity. |
| first_indexed | 2024-03-10T00:40:19Z |
| format | Article |
| id | doaj.art-6d9da87ebce543e1bab6e003a24cdb7d |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T00:40:19Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-6d9da87ebce543e1bab6e003a24cdb7d2023-11-23T15:08:55ZengMDPI AGPolymers2073-43602022-01-0114227710.3390/polym14020277Eugenyl-2-Hydroxypropyl Methacrylate-Incorporated Experimental Dental Composite: Degree of Polymerization and In Vitro Cytotoxicity EvaluationAbdel-Basit Al-Odayni0Dalal H. Alotaibi1Waseem Sharaf Saeed2Abdullah Al-Kahtani3Ali Assiri4Fahad M. Alkhtani5Ali Alrahlah6Engineer Abdullah Bugshan Research Chair for Dental and Oral Rehabilitation, College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaDepartment of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaEngineer Abdullah Bugshan Research Chair for Dental and Oral Rehabilitation, College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaChemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaCollege of Dentistry Research Center (CDRC), College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaDepartment of Prosthodontics, College of Dentistry, Prince Sattam Bin Abdulaziz University, Alkharj 11924, Saudi ArabiaEngineer Abdullah Bugshan Research Chair for Dental and Oral Rehabilitation, College of Dentistry, King Saud University, Riyadh 11545, Saudi ArabiaThe aim of this study was to evaluate the properties of new dental formulations containing eugenyl-2-hydroxypropyl methacrylate (EgGMA) monomer, as restorative dental material, in terms of their degree of photopolymerization and cytotoxicity. The target model composites (TBEg0, TBEg2.5, and TBEg5) were prepared by mixing 35% organic matrix (TEGDMA/BisGMA (50/50 wt%) of which 0, 2.5, and 5 wt%, respectively, were replaced with EgGMA monomer) with 65% filler (silanized hydroxyapatite (HA)/zinc oxide (ZnO<sub>2</sub>), 4:3 by weight). The vinylic double-bond conversion (DC) after light-curing was studied using Fourier transform infrared technique whereas cell viability was in vitro tested using primary human gingival fibroblasts cells over 7 days by means of AlamarBlue colorimetric assay. The obtained data were statistically analyzed using ANOVA and Tukey post-hoc tests. The results revealed no significant difference in DC between TBEg2.5 (66.49%) and control (TBEg0; 68.74%), whereas both differ significantly with TBEg5, likely due to the inhibitory effect of eugenol moiety at high concentration. The cell viability test indicated that all the composites are biocompatible. No significant difference was counted between TBEg2.5 and TBEg5, however, both differed significantly from the control (TBEg0). Thus, even though its apparent negative effect on polymerization, EgGMA is potentially safer than bisphenol-derived monomers. Such potential properties may encourage further investigations on term of EgGMA amount optimization, compatibility with other dental resins, and antimicrobial activity.https://www.mdpi.com/2073-4360/14/2/277eugenol derivativepolymerizable eugenoldental compositecytotoxicity |
| spellingShingle | Abdel-Basit Al-Odayni Dalal H. Alotaibi Waseem Sharaf Saeed Abdullah Al-Kahtani Ali Assiri Fahad M. Alkhtani Ali Alrahlah Eugenyl-2-Hydroxypropyl Methacrylate-Incorporated Experimental Dental Composite: Degree of Polymerization and In Vitro Cytotoxicity Evaluation Polymers eugenol derivative polymerizable eugenol dental composite cytotoxicity |
| title | Eugenyl-2-Hydroxypropyl Methacrylate-Incorporated Experimental Dental Composite: Degree of Polymerization and In Vitro Cytotoxicity Evaluation |
| title_full | Eugenyl-2-Hydroxypropyl Methacrylate-Incorporated Experimental Dental Composite: Degree of Polymerization and In Vitro Cytotoxicity Evaluation |
| title_fullStr | Eugenyl-2-Hydroxypropyl Methacrylate-Incorporated Experimental Dental Composite: Degree of Polymerization and In Vitro Cytotoxicity Evaluation |
| title_full_unstemmed | Eugenyl-2-Hydroxypropyl Methacrylate-Incorporated Experimental Dental Composite: Degree of Polymerization and In Vitro Cytotoxicity Evaluation |
| title_short | Eugenyl-2-Hydroxypropyl Methacrylate-Incorporated Experimental Dental Composite: Degree of Polymerization and In Vitro Cytotoxicity Evaluation |
| title_sort | eugenyl 2 hydroxypropyl methacrylate incorporated experimental dental composite degree of polymerization and in vitro cytotoxicity evaluation |
| topic | eugenol derivative polymerizable eugenol dental composite cytotoxicity |
| url | https://www.mdpi.com/2073-4360/14/2/277 |
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