The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle Cells
Plasma factor XIII (pFXIII) is a heterotetramer of FXIII-A and FXIII-B subunits. The cellular form (cFXIII), a dimer of FXIII-A, is present in a number of cell types. Activated FXIII (FXIIIa), a transglutaminase, plays an important role in clot stabilization, wound healing, angiogenesis and maintena...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5845 |
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| author | Réka Bogáti Éva Katona Amir H. Shemirani Enikő Balogh Helga Bárdos Viktória Jeney László Muszbek |
| author_facet | Réka Bogáti Éva Katona Amir H. Shemirani Enikő Balogh Helga Bárdos Viktória Jeney László Muszbek |
| author_sort | Réka Bogáti |
| collection | DOAJ |
| description | Plasma factor XIII (pFXIII) is a heterotetramer of FXIII-A and FXIII-B subunits. The cellular form (cFXIII), a dimer of FXIII-A, is present in a number of cell types. Activated FXIII (FXIIIa), a transglutaminase, plays an important role in clot stabilization, wound healing, angiogenesis and maintenance of pregnancy. It has a direct effect on vascular endothelial cells and fibroblasts, which have been implicated in the development of atherosclerotic plaques. Our aim was to explore the effect of FXIIIa on human aortic smooth muscle cells (HAoSMCs), another major cell type in the atherosclerotic plaque. Osteoblastic transformation induced by Pi and Ca<sup>2+</sup> failed to elicit the expression of cFXIII in HAoSMCs. EZ4U, CCK-8 and CytoSelect Wound Healing assays were used to investigate cell proliferation and migration. The Sircol Collagen Assay Kit was used to monitor collagen secretion. Thrombospondin-1 (TSP-1) levels were measured by ELISA. Cell-associated TSP-1 was detected by the immunofluorescence technique. The TSP-1 mRNA level was estimated by RT-qPCR. Activated recombinant cFXIII (rFXIIIa) increased cell proliferation and collagen secretion. In parallel, a 67% decrease in TSP-1 concentration in the medium and a 2.5-fold increase in cells were observed. TSP-1 mRNA did not change significantly. These effects of FXIIIa might contribute to the pathogenesis of atherosclerotic plaques. |
| first_indexed | 2024-03-10T03:42:30Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:42:30Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6d9dc0d3cd7744d7a205f0de512af8632023-11-23T11:29:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310584510.3390/ijms23105845The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle CellsRéka Bogáti0Éva Katona1Amir H. Shemirani2Enikő Balogh3Helga Bárdos4Viktória Jeney5László Muszbek6Division of Clinical Laboratory Science, Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, 98 Nagyerdei Krt, H-4032 Debrecen, HungaryDivision of Clinical Laboratory Science, Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, 98 Nagyerdei Krt, H-4032 Debrecen, HungaryDivision of Clinical Laboratory Science, Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, 98 Nagyerdei Krt, H-4032 Debrecen, HungaryResearch Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, 98 Nagyerdei Krt, H-4032 Debrecen, HungaryDepartment of Public Health and Epidemiology, Faculty of Medicine, University of Debrecen, Egyetem Tér 1, H-4032 Debrecen, HungaryResearch Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, 98 Nagyerdei Krt, H-4032 Debrecen, HungaryDivision of Clinical Laboratory Science, Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, 98 Nagyerdei Krt, H-4032 Debrecen, HungaryPlasma factor XIII (pFXIII) is a heterotetramer of FXIII-A and FXIII-B subunits. The cellular form (cFXIII), a dimer of FXIII-A, is present in a number of cell types. Activated FXIII (FXIIIa), a transglutaminase, plays an important role in clot stabilization, wound healing, angiogenesis and maintenance of pregnancy. It has a direct effect on vascular endothelial cells and fibroblasts, which have been implicated in the development of atherosclerotic plaques. Our aim was to explore the effect of FXIIIa on human aortic smooth muscle cells (HAoSMCs), another major cell type in the atherosclerotic plaque. Osteoblastic transformation induced by Pi and Ca<sup>2+</sup> failed to elicit the expression of cFXIII in HAoSMCs. EZ4U, CCK-8 and CytoSelect Wound Healing assays were used to investigate cell proliferation and migration. The Sircol Collagen Assay Kit was used to monitor collagen secretion. Thrombospondin-1 (TSP-1) levels were measured by ELISA. Cell-associated TSP-1 was detected by the immunofluorescence technique. The TSP-1 mRNA level was estimated by RT-qPCR. Activated recombinant cFXIII (rFXIIIa) increased cell proliferation and collagen secretion. In parallel, a 67% decrease in TSP-1 concentration in the medium and a 2.5-fold increase in cells were observed. TSP-1 mRNA did not change significantly. These effects of FXIIIa might contribute to the pathogenesis of atherosclerotic plaques.https://www.mdpi.com/1422-0067/23/10/5845factor XIIIvascular smooth muscle cellcell proliferationcollagen secretionthrombospondin-1 |
| spellingShingle | Réka Bogáti Éva Katona Amir H. Shemirani Enikő Balogh Helga Bárdos Viktória Jeney László Muszbek The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle Cells International Journal of Molecular Sciences factor XIII vascular smooth muscle cell cell proliferation collagen secretion thrombospondin-1 |
| title | The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle Cells |
| title_full | The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle Cells |
| title_fullStr | The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle Cells |
| title_full_unstemmed | The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle Cells |
| title_short | The Effect of Activated FXIII, a Transglutaminase, on Vascular Smooth Muscle Cells |
| title_sort | effect of activated fxiii a transglutaminase on vascular smooth muscle cells |
| topic | factor XIII vascular smooth muscle cell cell proliferation collagen secretion thrombospondin-1 |
| url | https://www.mdpi.com/1422-0067/23/10/5845 |
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