Characterization of the complete mitochondrial genome of Spirobolus grahami (Diplopoda: Spirobolidae) with phylogenetic analysis
Abstract Diplopoda is one of the most diverse and important groups of soil arthropods, but little research has been done on their phylogenetic relationship and evolution. Here, we sequenced and annotated the complete mitochondrial genomes of Spirobolus grahami. The total mitogenome of S. grahami was...
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-57421-3 |
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| author | Wenwen Zhang Tianyi Gan Tangjun Xu Peng Wang Jingzhe Tai Fangzhou Ma |
| author_facet | Wenwen Zhang Tianyi Gan Tangjun Xu Peng Wang Jingzhe Tai Fangzhou Ma |
| author_sort | Wenwen Zhang |
| collection | DOAJ |
| description | Abstract Diplopoda is one of the most diverse and important groups of soil arthropods, but little research has been done on their phylogenetic relationship and evolution. Here, we sequenced and annotated the complete mitochondrial genomes of Spirobolus grahami. The total mitogenome of S. grahami was typical circular, double-stranded molecules, with 14,875 bp in length, including 13 protein-coding genes, 22 tRNAs, two rRNAs, and one control region. Base composition analysis suggested that the mitochondrial sequences were biased toward A and T, with A + T content of 58.68%. The mitogenomes of S. grahami exhibited negative AT and positive GC skews. Most of the 13 PCGs had ATN as the start codon, except COX1 start with CGA, and most PCGs ended with the T stop codon. The dN/dS values for most PCGs were lower than 1, suggesting that purifying selection was likely the main driver of mitochondrial PCG evolution. Phylogenetic analyses based on 13 PCGs using BI and ML methods support the classification of genus Spirobolus and Tropostreptus. Glomeridesmus spelaeus is distantly related to the other Diplopoda species. |
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| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T16:19:47Z |
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| spelling | doaj.art-6d9dc467efbe436eab11c09e9972f8762024-03-31T11:16:53ZengNature PortfolioScientific Reports2045-23222024-03-011411910.1038/s41598-024-57421-3Characterization of the complete mitochondrial genome of Spirobolus grahami (Diplopoda: Spirobolidae) with phylogenetic analysisWenwen Zhang0Tianyi Gan1Tangjun Xu2Peng Wang3Jingzhe Tai4Fangzhou Ma5Research Center for Biodiversity Conservation and Biosafety/State Environmental Protection Scientific Observation and Research Station for Ecological Environment of Wuyi Mountains/Biodiversity Comprehensive Observation Station for Wuyi Mountains/State Environmental Protection Key Laboratory on Biosafety, Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment of ChinaCollege of Plant Health and Medicine, Qingdao Agricultural UniversityCollege of Life Science, Nanjing Forestry UniversityCollege of Plant Health and Medicine, Qingdao Agricultural UniversityCollege of Life Science, Nanjing Forestry UniversityResearch Center for Biodiversity Conservation and Biosafety/State Environmental Protection Scientific Observation and Research Station for Ecological Environment of Wuyi Mountains/Biodiversity Comprehensive Observation Station for Wuyi Mountains/State Environmental Protection Key Laboratory on Biosafety, Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment of ChinaAbstract Diplopoda is one of the most diverse and important groups of soil arthropods, but little research has been done on their phylogenetic relationship and evolution. Here, we sequenced and annotated the complete mitochondrial genomes of Spirobolus grahami. The total mitogenome of S. grahami was typical circular, double-stranded molecules, with 14,875 bp in length, including 13 protein-coding genes, 22 tRNAs, two rRNAs, and one control region. Base composition analysis suggested that the mitochondrial sequences were biased toward A and T, with A + T content of 58.68%. The mitogenomes of S. grahami exhibited negative AT and positive GC skews. Most of the 13 PCGs had ATN as the start codon, except COX1 start with CGA, and most PCGs ended with the T stop codon. The dN/dS values for most PCGs were lower than 1, suggesting that purifying selection was likely the main driver of mitochondrial PCG evolution. Phylogenetic analyses based on 13 PCGs using BI and ML methods support the classification of genus Spirobolus and Tropostreptus. Glomeridesmus spelaeus is distantly related to the other Diplopoda species.https://doi.org/10.1038/s41598-024-57421-3Mitochondrial genomeDiplopodaSpirobolus grahamiPhylogenetic analysis |
| spellingShingle | Wenwen Zhang Tianyi Gan Tangjun Xu Peng Wang Jingzhe Tai Fangzhou Ma Characterization of the complete mitochondrial genome of Spirobolus grahami (Diplopoda: Spirobolidae) with phylogenetic analysis Scientific Reports Mitochondrial genome Diplopoda Spirobolus grahami Phylogenetic analysis |
| title | Characterization of the complete mitochondrial genome of Spirobolus grahami (Diplopoda: Spirobolidae) with phylogenetic analysis |
| title_full | Characterization of the complete mitochondrial genome of Spirobolus grahami (Diplopoda: Spirobolidae) with phylogenetic analysis |
| title_fullStr | Characterization of the complete mitochondrial genome of Spirobolus grahami (Diplopoda: Spirobolidae) with phylogenetic analysis |
| title_full_unstemmed | Characterization of the complete mitochondrial genome of Spirobolus grahami (Diplopoda: Spirobolidae) with phylogenetic analysis |
| title_short | Characterization of the complete mitochondrial genome of Spirobolus grahami (Diplopoda: Spirobolidae) with phylogenetic analysis |
| title_sort | characterization of the complete mitochondrial genome of spirobolus grahami diplopoda spirobolidae with phylogenetic analysis |
| topic | Mitochondrial genome Diplopoda Spirobolus grahami Phylogenetic analysis |
| url | https://doi.org/10.1038/s41598-024-57421-3 |
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