Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas Validation of an UV spectrophotometric method for quantification of famotidine in capsules
<abstract language="eng">This work describes the validation of a method for quantification of famotidine in capsules by UV spectrophotometry using as solvents buffer solution at pH 2.5 and buffer solution at pH 4.5. The results show that the method is practical, selective, accurate,...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Química
2010-01-01
|
| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700028 |
| _version_ | 1818364928931659776 |
|---|---|
| author | Lívia Botacini Favoretto Juliana Maria Oliveira Souza Rudy Bonfilio Roberta de Cássia Pimentel Azevedo Magali Benjamim de Araújo |
| author_facet | Lívia Botacini Favoretto Juliana Maria Oliveira Souza Rudy Bonfilio Roberta de Cássia Pimentel Azevedo Magali Benjamim de Araújo |
| author_sort | Lívia Botacini Favoretto |
| collection | DOAJ |
| description | <abstract language="eng">This work describes the validation of a method for quantification of famotidine in capsules by UV spectrophotometry using as solvents buffer solution at pH 2.5 and buffer solution at pH 4.5. The results show that the method is practical, selective, accurate, precise and linear from 10.0 to 25.0 µg/mL of famotidine in both solvents. However, a variance analysis showed a lower mean percentage of famotidine when buffer at pH 2.5 is used. This effect was attributed to the instability of famotidine in acidic media. Therefore, the use of pH 4.5 buffer was considered suitable for analysis of famotidine in capsules. |
| first_indexed | 2024-12-13T22:12:10Z |
| format | Article |
| id | doaj.art-6d9f9f363f1b429a82f1150b308e8fdd |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-12-13T22:12:10Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-6d9f9f363f1b429a82f1150b308e8fdd2022-12-21T23:29:42ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642010-01-013371585158910.1590/S0100-40422010000700028Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas Validation of an UV spectrophotometric method for quantification of famotidine in capsulesLívia Botacini FavorettoJuliana Maria Oliveira SouzaRudy BonfilioRoberta de Cássia Pimentel AzevedoMagali Benjamim de Araújo<abstract language="eng">This work describes the validation of a method for quantification of famotidine in capsules by UV spectrophotometry using as solvents buffer solution at pH 2.5 and buffer solution at pH 4.5. The results show that the method is practical, selective, accurate, precise and linear from 10.0 to 25.0 µg/mL of famotidine in both solvents. However, a variance analysis showed a lower mean percentage of famotidine when buffer at pH 2.5 is used. This effect was attributed to the instability of famotidine in acidic media. Therefore, the use of pH 4.5 buffer was considered suitable for analysis of famotidine in capsules.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700028famotidinevalidationUV spectrophotometry |
| spellingShingle | Lívia Botacini Favoretto Juliana Maria Oliveira Souza Rudy Bonfilio Roberta de Cássia Pimentel Azevedo Magali Benjamim de Araújo Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas Validation of an UV spectrophotometric method for quantification of famotidine in capsules Química Nova famotidine validation UV spectrophotometry |
| title | Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas Validation of an UV spectrophotometric method for quantification of famotidine in capsules |
| title_full | Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas Validation of an UV spectrophotometric method for quantification of famotidine in capsules |
| title_fullStr | Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas Validation of an UV spectrophotometric method for quantification of famotidine in capsules |
| title_full_unstemmed | Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas Validation of an UV spectrophotometric method for quantification of famotidine in capsules |
| title_short | Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas Validation of an UV spectrophotometric method for quantification of famotidine in capsules |
| title_sort | validacao de metodo espectrofotometrico na regiao do uv para quantificacao de famotidina em capsulas validation of an uv spectrophotometric method for quantification of famotidine in capsules |
| topic | famotidine validation UV spectrophotometry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700028 |
| work_keys_str_mv | AT liviabotacinifavoretto validacaodemetodoespectrofotometriconaregiaodouvparaquantificacaodefamotidinaemcapsulasvalidationofanuvspectrophotometricmethodforquantificationoffamotidineincapsules AT julianamariaoliveirasouza validacaodemetodoespectrofotometriconaregiaodouvparaquantificacaodefamotidinaemcapsulasvalidationofanuvspectrophotometricmethodforquantificationoffamotidineincapsules AT rudybonfilio validacaodemetodoespectrofotometriconaregiaodouvparaquantificacaodefamotidinaemcapsulasvalidationofanuvspectrophotometricmethodforquantificationoffamotidineincapsules AT robertadecassiapimentelazevedo validacaodemetodoespectrofotometriconaregiaodouvparaquantificacaodefamotidinaemcapsulasvalidationofanuvspectrophotometricmethodforquantificationoffamotidineincapsules AT magalibenjamimdearaujo validacaodemetodoespectrofotometriconaregiaodouvparaquantificacaodefamotidinaemcapsulasvalidationofanuvspectrophotometricmethodforquantificationoffamotidineincapsules |