An Electrochemical Nano-immunosensor for Rapid Detection of α-Casein in Milk
Based on electrochemical biosensing technology, chitosan was used as bridge agent, combined with gold nanoparticles and horseradish peroxidase electrical signal amplification system, and FcεRI receptor protein was used as general molecular probe to adsorb anti-bovine milk α-casein antibody IgE, cons...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2022-08-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110173 |
| _version_ | 1798017584309731328 |
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| author | Dandan PENG Danyang LIU Lina LI Zeyang LIU Dingqiang LU Guangchang PANG |
| author_facet | Dandan PENG Danyang LIU Lina LI Zeyang LIU Dingqiang LU Guangchang PANG |
| author_sort | Dandan PENG |
| collection | DOAJ |
| description | Based on electrochemical biosensing technology, chitosan was used as bridge agent, combined with gold nanoparticles and horseradish peroxidase electrical signal amplification system, and FcεRI receptor protein was used as general molecular probe to adsorb anti-bovine milk α-casein antibody IgE, constructed a nano-gold immunosensor for the detection of allergen α-casein in bovine milk, and the milk α-casein was verified by time-current curve. The results showed that the kinetic equation of α-casein-IgE (immunoglobulins E) interaction conforms to the hyperbolic law, the results met the hyperbolic fitting criterion R2≥0.95, and had a substrate saturation effect similar to that of enzyme-substrate interaction. The value of the α-casein allosteric interaction constant (Ka) was 4.096×10−12 mol/L calculated by referring to the Michaelis constant. The biosensor developed in this study could bind to the specific IgE of allergens and realize ultrasensitive detection of allergens. The detection method had high sensitivity, simple operation and low cost, and could provide a new detection method for allergic reactions caused by low-dose allergens. |
| first_indexed | 2024-04-11T16:09:38Z |
| format | Article |
| id | doaj.art-6da2578c501a42658926653d2be4404c |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:09:38Z |
| publishDate | 2022-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-6da2578c501a42658926653d2be4404c2022-12-22T04:14:43ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-08-01431630731410.13386/j.issn1002-0306.20211101732021110173-16An Electrochemical Nano-immunosensor for Rapid Detection of α-Casein in MilkDandan PENG0Danyang LIU1Lina LI2Zeyang LIU3Dingqiang LU4Guangchang PANG5School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaSchool of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaSchool of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaSchool of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaSchool of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaSchool of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, ChinaBased on electrochemical biosensing technology, chitosan was used as bridge agent, combined with gold nanoparticles and horseradish peroxidase electrical signal amplification system, and FcεRI receptor protein was used as general molecular probe to adsorb anti-bovine milk α-casein antibody IgE, constructed a nano-gold immunosensor for the detection of allergen α-casein in bovine milk, and the milk α-casein was verified by time-current curve. The results showed that the kinetic equation of α-casein-IgE (immunoglobulins E) interaction conforms to the hyperbolic law, the results met the hyperbolic fitting criterion R2≥0.95, and had a substrate saturation effect similar to that of enzyme-substrate interaction. The value of the α-casein allosteric interaction constant (Ka) was 4.096×10−12 mol/L calculated by referring to the Michaelis constant. The biosensor developed in this study could bind to the specific IgE of allergens and realize ultrasensitive detection of allergens. The detection method had high sensitivity, simple operation and low cost, and could provide a new detection method for allergic reactions caused by low-dose allergens.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110173electrochemical sensorα-caseinfcεri receptorgold nanoparticlesallergen detection |
| spellingShingle | Dandan PENG Danyang LIU Lina LI Zeyang LIU Dingqiang LU Guangchang PANG An Electrochemical Nano-immunosensor for Rapid Detection of α-Casein in Milk Shipin gongye ke-ji electrochemical sensor α-casein fcεri receptor gold nanoparticles allergen detection |
| title | An Electrochemical Nano-immunosensor for Rapid Detection of α-Casein in Milk |
| title_full | An Electrochemical Nano-immunosensor for Rapid Detection of α-Casein in Milk |
| title_fullStr | An Electrochemical Nano-immunosensor for Rapid Detection of α-Casein in Milk |
| title_full_unstemmed | An Electrochemical Nano-immunosensor for Rapid Detection of α-Casein in Milk |
| title_short | An Electrochemical Nano-immunosensor for Rapid Detection of α-Casein in Milk |
| title_sort | electrochemical nano immunosensor for rapid detection of α casein in milk |
| topic | electrochemical sensor α-casein fcεri receptor gold nanoparticles allergen detection |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110173 |
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