Characterization of Differentially Expressed Genes under Salt Stress in Olive
Climate change, currently taking place worldwide and also in the Mediterranean area, is leading to a reduction in water availability and to groundwater salinization. Olive represents one of the most efficient tree crops to face these scenarios, thanks to its natural ability to tolerate moderate sali...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/1/154 |
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| author | Soraya Mousavi Roberto Mariotti Maria Cristina Valeri Luca Regni Emanuele Lilli Emidio Albertini Primo Proietti Daniela Businelli Luciana Baldoni |
| author_facet | Soraya Mousavi Roberto Mariotti Maria Cristina Valeri Luca Regni Emanuele Lilli Emidio Albertini Primo Proietti Daniela Businelli Luciana Baldoni |
| author_sort | Soraya Mousavi |
| collection | DOAJ |
| description | Climate change, currently taking place worldwide and also in the Mediterranean area, is leading to a reduction in water availability and to groundwater salinization. Olive represents one of the most efficient tree crops to face these scenarios, thanks to its natural ability to tolerate moderate salinity and drought. In the present work, four olive cultivars (Koroneiki, Picual, Royal de Cazorla and Fadak86) were exposed to high salt stress conditions (200 mM of NaCl) in greenhouse, in order to evaluate their tolerance level and to identify key genes involved in salt stress response. Molecular and physiological parameters, as well as plant growth and leaves’ ions Na<sup>+</sup> and K<sup>+</sup> content were measured. Results of the physiological measurements showed Royal de Cazorla as the most tolerant cultivar, and Fadak86 and Picual as the most susceptible ones. Ten candidate genes were analyzed and their complete genomic, CDS and protein sequences were identified. The expression analysis of their transcripts through reverse transcriptase quantitative PCR (RT-qPCR) demonstrated that only <i>OeNHX7</i>, <i>OeP5CS</i>, <i>OeRD19A</i> and <i>OePetD</i> were upregulated in tolerant cultivars, thus suggesting their key role in the activation of a salt tolerance mechanism. |
| first_indexed | 2024-03-10T03:39:37Z |
| format | Article |
| id | doaj.art-6da3cdeb66d744e99a719c3f70e0e23a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:39:37Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6da3cdeb66d744e99a719c3f70e0e23a2023-11-23T11:35:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0123115410.3390/ijms23010154Characterization of Differentially Expressed Genes under Salt Stress in OliveSoraya Mousavi0Roberto Mariotti1Maria Cristina Valeri2Luca Regni3Emanuele Lilli4Emidio Albertini5Primo Proietti6Daniela Businelli7Luciana Baldoni8Institute of Biosciences and Bioresources, National Research Council, 06128 Perugia, ItalyInstitute of Biosciences and Bioresources, National Research Council, 06128 Perugia, ItalyInstitute of Biosciences and Bioresources, National Research Council, 06128 Perugia, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyInstitute of Biosciences and Bioresources, National Research Council, 06128 Perugia, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyInstitute of Biosciences and Bioresources, National Research Council, 06128 Perugia, ItalyClimate change, currently taking place worldwide and also in the Mediterranean area, is leading to a reduction in water availability and to groundwater salinization. Olive represents one of the most efficient tree crops to face these scenarios, thanks to its natural ability to tolerate moderate salinity and drought. In the present work, four olive cultivars (Koroneiki, Picual, Royal de Cazorla and Fadak86) were exposed to high salt stress conditions (200 mM of NaCl) in greenhouse, in order to evaluate their tolerance level and to identify key genes involved in salt stress response. Molecular and physiological parameters, as well as plant growth and leaves’ ions Na<sup>+</sup> and K<sup>+</sup> content were measured. Results of the physiological measurements showed Royal de Cazorla as the most tolerant cultivar, and Fadak86 and Picual as the most susceptible ones. Ten candidate genes were analyzed and their complete genomic, CDS and protein sequences were identified. The expression analysis of their transcripts through reverse transcriptase quantitative PCR (RT-qPCR) demonstrated that only <i>OeNHX7</i>, <i>OeP5CS</i>, <i>OeRD19A</i> and <i>OePetD</i> were upregulated in tolerant cultivars, thus suggesting their key role in the activation of a salt tolerance mechanism.https://www.mdpi.com/1422-0067/23/1/154salt stress genes<i>Olea europaea</i>gene expressionclimate changesalt toleranceolive cultivars |
| spellingShingle | Soraya Mousavi Roberto Mariotti Maria Cristina Valeri Luca Regni Emanuele Lilli Emidio Albertini Primo Proietti Daniela Businelli Luciana Baldoni Characterization of Differentially Expressed Genes under Salt Stress in Olive International Journal of Molecular Sciences salt stress genes <i>Olea europaea</i> gene expression climate change salt tolerance olive cultivars |
| title | Characterization of Differentially Expressed Genes under Salt Stress in Olive |
| title_full | Characterization of Differentially Expressed Genes under Salt Stress in Olive |
| title_fullStr | Characterization of Differentially Expressed Genes under Salt Stress in Olive |
| title_full_unstemmed | Characterization of Differentially Expressed Genes under Salt Stress in Olive |
| title_short | Characterization of Differentially Expressed Genes under Salt Stress in Olive |
| title_sort | characterization of differentially expressed genes under salt stress in olive |
| topic | salt stress genes <i>Olea europaea</i> gene expression climate change salt tolerance olive cultivars |
| url | https://www.mdpi.com/1422-0067/23/1/154 |
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