Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infection
Abstract Background Family with sequence similarity 26, member F (FAM26F) is an important innate immunity modulator playing a significant role in diverse immune responses, however, the association of FAM26F expression with HBV infection is not yet known. Thus, the current study aims to explore the d...
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-021-05888-0 |
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| author | Kehkshan Jabeen Uzma Malik Sajid Mansoor Shaheen Shahzad Saadia Zahid Aneela Javed |
| author_facet | Kehkshan Jabeen Uzma Malik Sajid Mansoor Shaheen Shahzad Saadia Zahid Aneela Javed |
| author_sort | Kehkshan Jabeen |
| collection | DOAJ |
| description | Abstract Background Family with sequence similarity 26, member F (FAM26F) is an important innate immunity modulator playing a significant role in diverse immune responses, however, the association of FAM26F expression with HBV infection is not yet known. Thus, the current study aims to explore the differential expression of FAM26F in vitro in HepAD38 and HepG2 cell lines upon HBV infection, and in vivo in HBV infected individuals. The effects of antioxidant and calcium inhibitors on the regulation of FAM26F expression were also evaluated. The expression of FAM26F was simultaneously determined with well-established HBV infection markers: IRF3, and IFN-β. Methods The expression of FAM26F and marker genes was analyzed through Real-time qPCR and western blot. Results Our results indicate that the differential expression of FAM26F followed the same trend as that of IRF3 and IFN-β. The in vitro study revealed that, in both HBV infected cell lines, FAM26F expression was significantly down-regulated as compared to uninfected control cells. Treatment of cells with N-acetyl-L-cysteine (NAC), EGTA-AM, BAPTA-AM, and Ru360 significantly upregulated the expression of FAM26F in both the cell lines. Moreover, in in vivo study, FAM26F expression was significantly downregulated in all HBV infected groups as compared to controls (p = 0.0007). The expression was higher in the HBV recovered cases, probably due to the decrease in infection and increase in the immunity of these individuals. Conclusion Our study is the first to show the association of FAM26F with HBV infection. It is proposed that FAM26F expression could be an early predictive marker for HBV infection, and thus is worthy of further investigation. |
| first_indexed | 2024-12-14T17:25:59Z |
| format | Article |
| id | doaj.art-6da68525a4c7493fbefe90f085649f18 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-14T17:25:59Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-6da68525a4c7493fbefe90f085649f182022-12-21T22:53:13ZengBMCBMC Infectious Diseases1471-23342021-02-0121111310.1186/s12879-021-05888-0Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infectionKehkshan Jabeen0Uzma Malik1Sajid Mansoor2Shaheen Shahzad3Saadia Zahid4Aneela Javed5Genomics Research Lab, Department of Biological Sciences, International Islamic University IslamabadDepartment of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST)Department of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST)Genomics Research Lab, Department of Biological Sciences, International Islamic University IslamabadDepartment of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST)Department of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST)Abstract Background Family with sequence similarity 26, member F (FAM26F) is an important innate immunity modulator playing a significant role in diverse immune responses, however, the association of FAM26F expression with HBV infection is not yet known. Thus, the current study aims to explore the differential expression of FAM26F in vitro in HepAD38 and HepG2 cell lines upon HBV infection, and in vivo in HBV infected individuals. The effects of antioxidant and calcium inhibitors on the regulation of FAM26F expression were also evaluated. The expression of FAM26F was simultaneously determined with well-established HBV infection markers: IRF3, and IFN-β. Methods The expression of FAM26F and marker genes was analyzed through Real-time qPCR and western blot. Results Our results indicate that the differential expression of FAM26F followed the same trend as that of IRF3 and IFN-β. The in vitro study revealed that, in both HBV infected cell lines, FAM26F expression was significantly down-regulated as compared to uninfected control cells. Treatment of cells with N-acetyl-L-cysteine (NAC), EGTA-AM, BAPTA-AM, and Ru360 significantly upregulated the expression of FAM26F in both the cell lines. Moreover, in in vivo study, FAM26F expression was significantly downregulated in all HBV infected groups as compared to controls (p = 0.0007). The expression was higher in the HBV recovered cases, probably due to the decrease in infection and increase in the immunity of these individuals. Conclusion Our study is the first to show the association of FAM26F with HBV infection. It is proposed that FAM26F expression could be an early predictive marker for HBV infection, and thus is worthy of further investigation.https://doi.org/10.1186/s12879-021-05888-0Family with sequence similarity 26 member F (FAM26F)Hepatitis B virus (HBV)Reactive oxygen species (ROS)Calcium deregulationCalcium chelatorsNAC |
| spellingShingle | Kehkshan Jabeen Uzma Malik Sajid Mansoor Shaheen Shahzad Saadia Zahid Aneela Javed Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infection BMC Infectious Diseases Family with sequence similarity 26 member F (FAM26F) Hepatitis B virus (HBV) Reactive oxygen species (ROS) Calcium deregulation Calcium chelators NAC |
| title | Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infection |
| title_full | Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infection |
| title_fullStr | Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infection |
| title_full_unstemmed | Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infection |
| title_short | Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infection |
| title_sort | effect of oxidative stress and calcium deregulation on fam26f calhm6 expression during hepatitis b virus infection |
| topic | Family with sequence similarity 26 member F (FAM26F) Hepatitis B virus (HBV) Reactive oxygen species (ROS) Calcium deregulation Calcium chelators NAC |
| url | https://doi.org/10.1186/s12879-021-05888-0 |
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