Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection Leishmania mexicana amazonensis: heterogeneidade da 5’-Nucleotidase e da peroxidase em fagócitos mononucleares durante infecção in vivo e in vitro
The degree of maturation of cells of the Mononuclear Phagocyte System (MPS), during in vivo and in vitro infection by Leishmania mexicana amazonenesis, was evaluated in this study. The macrophages' differentiation was assayed by cytochemical characterization at the ultrastrctural level, using t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1988-03-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100015 |
| _version_ | 1797718362272301056 |
|---|---|
| author | Suzana Côrte-Real Gabriel Grimaldi Junior Maria de Nazareth Leal de Meirelles |
| author_facet | Suzana Côrte-Real Gabriel Grimaldi Junior Maria de Nazareth Leal de Meirelles |
| author_sort | Suzana Côrte-Real |
| collection | DOAJ |
| description | The degree of maturation of cells of the Mononuclear Phagocyte System (MPS), during in vivo and in vitro infection by Leishmania mexicana amazonenesis, was evaluated in this study. The macrophages' differentiation was assayed by cytochemical characterization at the ultrastrctural level, using two well-established markers: 5'-nucleotidase enzyme activity, for revealing the mature cells, and the peroxidase activity present in the cell granules to demonstrate immature mononuclear phagocytes. only a few mcrophages, demonstrating 5'-nucleotidase positive reaction in both the plasma membrane and within their cytoplasmic vesicles, were found scattered in the chronic inflammation at the L. m. amazonensis lesions in albino mice. However, by the peroxidase activity analysis, we were also able to demonstrate the presence of immature MPS cells, which predominate, together with parasitized vacuolated macrophages, in chronic lesions induced in this systemby L. m. amazonensis. The implications of these results on the pathogenesis of murine cutaneous leishmaniasis are discussed.<br>Um estudo sobre o grau de maturação das células do Sistema Fagocítico Mononuclear foi realizado durante a infecção in vivo e in vitro com a Leishmania mexicana amazonensis. A caracterização da diferenciação das células fagocíticas foi obtida com a localização ultraestrutural de dois marcadores enzimáticos bam conhecidos: a enzima 5'-Nucleotidase marcadora de membrana plasmática de células maduras e a enzima peroxidase, presente em grânulos, marcadora de células imaturas. A atividade da enzima 5'-Nucleotidase foi encontrada apenas em alguns macrófagos, presentes no foco inflamatório, em projeções da membrana plasmática e em algumas vesículas citoplasmáticas. Macrófagos peritoneais de camundongo apresentaram a mesma reatividade para este marcador. Contudo a análise da atividade peroxidásica demonstrou a predominância da presença de fagócitos mononucleares imaturos nas lesões crônicas induzidas neste sistema por Leishmania mexicana amazonensis. |
| first_indexed | 2024-03-12T08:49:54Z |
| format | Article |
| id | doaj.art-6da9688ed9a145128f58e29640434be7 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:49:54Z |
| publishDate | 1988-03-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-6da9688ed9a145128f58e29640434be72023-09-02T16:21:37ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601988-03-0183111312110.1590/S0074-02761988000100015Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection Leishmania mexicana amazonensis: heterogeneidade da 5’-Nucleotidase e da peroxidase em fagócitos mononucleares durante infecção in vivo e in vitroSuzana Côrte-RealGabriel Grimaldi JuniorMaria de Nazareth Leal de MeirellesThe degree of maturation of cells of the Mononuclear Phagocyte System (MPS), during in vivo and in vitro infection by Leishmania mexicana amazonenesis, was evaluated in this study. The macrophages' differentiation was assayed by cytochemical characterization at the ultrastrctural level, using two well-established markers: 5'-nucleotidase enzyme activity, for revealing the mature cells, and the peroxidase activity present in the cell granules to demonstrate immature mononuclear phagocytes. only a few mcrophages, demonstrating 5'-nucleotidase positive reaction in both the plasma membrane and within their cytoplasmic vesicles, were found scattered in the chronic inflammation at the L. m. amazonensis lesions in albino mice. However, by the peroxidase activity analysis, we were also able to demonstrate the presence of immature MPS cells, which predominate, together with parasitized vacuolated macrophages, in chronic lesions induced in this systemby L. m. amazonensis. The implications of these results on the pathogenesis of murine cutaneous leishmaniasis are discussed.<br>Um estudo sobre o grau de maturação das células do Sistema Fagocítico Mononuclear foi realizado durante a infecção in vivo e in vitro com a Leishmania mexicana amazonensis. A caracterização da diferenciação das células fagocíticas foi obtida com a localização ultraestrutural de dois marcadores enzimáticos bam conhecidos: a enzima 5'-Nucleotidase marcadora de membrana plasmática de células maduras e a enzima peroxidase, presente em grânulos, marcadora de células imaturas. A atividade da enzima 5'-Nucleotidase foi encontrada apenas em alguns macrófagos, presentes no foco inflamatório, em projeções da membrana plasmática e em algumas vesículas citoplasmáticas. Macrófagos peritoneais de camundongo apresentaram a mesma reatividade para este marcador. Contudo a análise da atividade peroxidásica demonstrou a predominância da presença de fagócitos mononucleares imaturos nas lesões crônicas induzidas neste sistema por Leishmania mexicana amazonensis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100015Leishmania mexicana amazonensis (L. m. amazonensis)fagócito mononuclear imaturomacrófago maturomacrófago peritoneallesões cutâneas5'Nucleotidase (E. C. 3. 1. 3. 5)Peroxidase (E. C. 1. 11. 1. 7)Leishmania mexicana amazonensis (L. m. amazonensis)immature mononuclear phagocytemature macrophagesperiotoneal macrophagescutaneous lesions5'Nucleotidase (E. C. 3. 1. 3. 5)Peroxidase (E. C. 1. 11. 1. 7) |
| spellingShingle | Suzana Côrte-Real Gabriel Grimaldi Junior Maria de Nazareth Leal de Meirelles Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection Leishmania mexicana amazonensis: heterogeneidade da 5’-Nucleotidase e da peroxidase em fagócitos mononucleares durante infecção in vivo e in vitro Memorias do Instituto Oswaldo Cruz Leishmania mexicana amazonensis (L. m. amazonensis) fagócito mononuclear imaturo macrófago maturo macrófago peritoneal lesões cutâneas 5'Nucleotidase (E. C. 3. 1. 3. 5) Peroxidase (E. C. 1. 11. 1. 7) Leishmania mexicana amazonensis (L. m. amazonensis) immature mononuclear phagocyte mature macrophages periotoneal macrophages cutaneous lesions 5'Nucleotidase (E. C. 3. 1. 3. 5) Peroxidase (E. C. 1. 11. 1. 7) |
| title | Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection Leishmania mexicana amazonensis: heterogeneidade da 5’-Nucleotidase e da peroxidase em fagócitos mononucleares durante infecção in vivo e in vitro |
| title_full | Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection Leishmania mexicana amazonensis: heterogeneidade da 5’-Nucleotidase e da peroxidase em fagócitos mononucleares durante infecção in vivo e in vitro |
| title_fullStr | Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection Leishmania mexicana amazonensis: heterogeneidade da 5’-Nucleotidase e da peroxidase em fagócitos mononucleares durante infecção in vivo e in vitro |
| title_full_unstemmed | Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection Leishmania mexicana amazonensis: heterogeneidade da 5’-Nucleotidase e da peroxidase em fagócitos mononucleares durante infecção in vivo e in vitro |
| title_short | Leishmania mexicana amazonensis: heterogeneity in 5-nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection Leishmania mexicana amazonensis: heterogeneidade da 5’-Nucleotidase e da peroxidase em fagócitos mononucleares durante infecção in vivo e in vitro |
| title_sort | leishmania mexicana amazonensis heterogeneity in 5 nucleotidase and peroxidase activities of mononuclear phagocytes during in vivo and in vitro infection leishmania mexicana amazonensis heterogeneidade da 5 nucleotidase e da peroxidase em fagocitos mononucleares durante infeccao in vivo e in vitro |
| topic | Leishmania mexicana amazonensis (L. m. amazonensis) fagócito mononuclear imaturo macrófago maturo macrófago peritoneal lesões cutâneas 5'Nucleotidase (E. C. 3. 1. 3. 5) Peroxidase (E. C. 1. 11. 1. 7) Leishmania mexicana amazonensis (L. m. amazonensis) immature mononuclear phagocyte mature macrophages periotoneal macrophages cutaneous lesions 5'Nucleotidase (E. C. 3. 1. 3. 5) Peroxidase (E. C. 1. 11. 1. 7) |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100015 |
| work_keys_str_mv | AT suzanacortereal leishmaniamexicanaamazonensisheterogeneityin5nucleotidaseandperoxidaseactivitiesofmononuclearphagocytesduringinvivoandinvitroinfectionleishmaniamexicanaamazonensisheterogeneidadeda5nucleotidaseedaperoxidaseemfagocitosmononuclearesduranteinfeccaoinvivoeinv AT gabrielgrimaldijunior leishmaniamexicanaamazonensisheterogeneityin5nucleotidaseandperoxidaseactivitiesofmononuclearphagocytesduringinvivoandinvitroinfectionleishmaniamexicanaamazonensisheterogeneidadeda5nucleotidaseedaperoxidaseemfagocitosmononuclearesduranteinfeccaoinvivoeinv AT mariadenazarethlealdemeirelles leishmaniamexicanaamazonensisheterogeneityin5nucleotidaseandperoxidaseactivitiesofmononuclearphagocytesduringinvivoandinvitroinfectionleishmaniamexicanaamazonensisheterogeneidadeda5nucleotidaseedaperoxidaseemfagocitosmononuclearesduranteinfeccaoinvivoeinv |