MYC Participates In Lipopolysaccharide-Induced Sepsis Via Promoting Cell Proliferation And Inhibiting Apoptosis
Objective This study aimed to explore the potential mechanism of MYC proto-oncogene, BHLH Transcription Factor (MYC) gene, on sepsis. Materials And Methods In this experimental study, rat-derived H9C2 cardiomyocyte cells were cultured in vitro, followed by lipopolysaccharide (LPS) treatment wit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2020-09-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://celljournal.org/journal/article/fulltext/myc-participates-in-lps-induced-sepsis-via-promoting-cell-proliferation-and-inhibiting-apoptosis.pdf |
| _version_ | 1818060376770609152 |
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| author | Yin Li Chengqi Kong Lei Feng Wenliang Tang Mengwei Chen Zhiyuan Zheng |
| author_facet | Yin Li Chengqi Kong Lei Feng Wenliang Tang Mengwei Chen Zhiyuan Zheng |
| author_sort | Yin Li |
| collection | DOAJ |
| description | Objective
This study aimed to explore the potential mechanism of MYC proto-oncogene, BHLH Transcription Factor (MYC) gene, on sepsis.
Materials And Methods
In this experimental study, rat-derived H9C2 cardiomyocyte cells were cultured in vitro, followed by lipopolysaccharide (LPS) treatment with different concentration gradients. The cholecystokinin octapeptide (CCK-8) assay, enzyme-linked immunoassay (ELISA) assay, quantitative reverse transcription polymerase chain reaction (qRT-PCR), cell transfection, Western blot and flow cytometry were used to observe the cellular apoptosis and proliferation of cells in both treated LPS groups and normal control group.
Results
The result of CCK-8 assay showed that silencing MYC inhibited cellular proliferation of sepsis in absence or presence of LPS treatment. ELISA assay showed that the expressions of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were decreased in MYC silenced group, but they were increased after LPS treatment. Moreover, Flow cytometry assay showed that MYC silencing contributed to the apoptosis of sepsis cells. Furthermore, the expression of inflammatory factors showed that MYC silencing elevated the expression of inflammation factors.
Conclusion
MYC might take part in the process of LPS induced sepsis through suppressing apoptosis and inducing cell proliferation. Moreover, MYC might reduce inflammation during the progression of LPS induced sepsis. |
| first_indexed | 2024-12-10T13:31:26Z |
| format | Article |
| id | doaj.art-6dac171eebf14793ae4410176cdc81e9 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-10T13:31:26Z |
| publishDate | 2020-09-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-6dac171eebf14793ae4410176cdc81e92022-12-22T01:46:58ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142020-09-0122suppl 1687310.22074/cellj.2020.6961MYC Participates In Lipopolysaccharide-Induced Sepsis Via Promoting Cell Proliferation And Inhibiting ApoptosisYin Li0Chengqi Kong1Lei Feng2Wenliang Tang3Mengwei Chen4Zhiyuan Zheng5Emergency Department of Huadong Hospital, Fudan University, Yan’an Xi Road, Shanghai, ChinaCardiovascular Department of Huadong Hospital, Fudan University, Shanghai, ChinaEmergency Department of Huadong Hospital, Fudan University, Yan’an Xi Road, Shanghai, ChinaEmergency Department of Huadong Hospital, Fudan University, Yan’an Xi Road, Shanghai, ChinaCardiovascular Department of Huadong Hospital, Fudan University, Shanghai, ChinaCardiovascular Department of Huadong Hospital, Fudan University, Shanghai, China. Emails:andychenmw@hotmail.com,dora_zzy@hotmail.comObjective This study aimed to explore the potential mechanism of MYC proto-oncogene, BHLH Transcription Factor (MYC) gene, on sepsis. Materials And Methods In this experimental study, rat-derived H9C2 cardiomyocyte cells were cultured in vitro, followed by lipopolysaccharide (LPS) treatment with different concentration gradients. The cholecystokinin octapeptide (CCK-8) assay, enzyme-linked immunoassay (ELISA) assay, quantitative reverse transcription polymerase chain reaction (qRT-PCR), cell transfection, Western blot and flow cytometry were used to observe the cellular apoptosis and proliferation of cells in both treated LPS groups and normal control group. Results The result of CCK-8 assay showed that silencing MYC inhibited cellular proliferation of sepsis in absence or presence of LPS treatment. ELISA assay showed that the expressions of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were decreased in MYC silenced group, but they were increased after LPS treatment. Moreover, Flow cytometry assay showed that MYC silencing contributed to the apoptosis of sepsis cells. Furthermore, the expression of inflammatory factors showed that MYC silencing elevated the expression of inflammation factors. Conclusion MYC might take part in the process of LPS induced sepsis through suppressing apoptosis and inducing cell proliferation. Moreover, MYC might reduce inflammation during the progression of LPS induced sepsis.https://celljournal.org/journal/article/fulltext/myc-participates-in-lps-induced-sepsis-via-promoting-cell-proliferation-and-inhibiting-apoptosis.pdfcell apoptosiscell proliferationinflammationlipopolysaccharidemycsepsis |
| spellingShingle | Yin Li Chengqi Kong Lei Feng Wenliang Tang Mengwei Chen Zhiyuan Zheng MYC Participates In Lipopolysaccharide-Induced Sepsis Via Promoting Cell Proliferation And Inhibiting Apoptosis Cell Journal cell apoptosis cell proliferation inflammation lipopolysaccharide myc sepsis |
| title | MYC Participates In Lipopolysaccharide-Induced Sepsis Via Promoting Cell Proliferation And Inhibiting Apoptosis |
| title_full | MYC Participates In Lipopolysaccharide-Induced Sepsis Via Promoting Cell Proliferation And Inhibiting Apoptosis |
| title_fullStr | MYC Participates In Lipopolysaccharide-Induced Sepsis Via Promoting Cell Proliferation And Inhibiting Apoptosis |
| title_full_unstemmed | MYC Participates In Lipopolysaccharide-Induced Sepsis Via Promoting Cell Proliferation And Inhibiting Apoptosis |
| title_short | MYC Participates In Lipopolysaccharide-Induced Sepsis Via Promoting Cell Proliferation And Inhibiting Apoptosis |
| title_sort | myc participates in lipopolysaccharide induced sepsis via promoting cell proliferation and inhibiting apoptosis |
| topic | cell apoptosis cell proliferation inflammation lipopolysaccharide myc sepsis |
| url | https://celljournal.org/journal/article/fulltext/myc-participates-in-lps-induced-sepsis-via-promoting-cell-proliferation-and-inhibiting-apoptosis.pdf |
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