Molecular characterization of the genome-wide BOR transporter family and their responses to boron conditions in common wheat (Triticum aestivum L.)
Boron (B) deficiency is an agricultural problem that causes significant yield losses in many countries. B transporters (BORs) are responsible for B uptake and distribution and play important roles in yield formation. A comprehensive analysis of the BOR family members in common wheat is still lacking...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.997915/full |
| _version_ | 1817982334159290368 |
|---|---|
| author | Yuquan Wang Yuquan Wang Zhipeng Niu Zhipeng Niu Xigui Hu Xigui Hu Xiaojun Wu Xiaojun Wu Zijun Yang Zijun Yang Chenyan Hao Chenyan Hao Mengxue Zhou Mengxue Zhou Shumin Yang Shumin Yang Na Dong Na Dong Mingjiu Liu Mingjiu Liu Zhengang Ru Zhengang Ru |
| author_facet | Yuquan Wang Yuquan Wang Zhipeng Niu Zhipeng Niu Xigui Hu Xigui Hu Xiaojun Wu Xiaojun Wu Zijun Yang Zijun Yang Chenyan Hao Chenyan Hao Mengxue Zhou Mengxue Zhou Shumin Yang Shumin Yang Na Dong Na Dong Mingjiu Liu Mingjiu Liu Zhengang Ru Zhengang Ru |
| author_sort | Yuquan Wang |
| collection | DOAJ |
| description | Boron (B) deficiency is an agricultural problem that causes significant yield losses in many countries. B transporters (BORs) are responsible for B uptake and distribution and play important roles in yield formation. A comprehensive analysis of the BOR family members in common wheat is still lacking. In the present study, to clarify the molecular characterization and response to B status, genome-wide TaBOR genes and expression patterns were investigated. Fourteen TaBOR genes were identified in common wheat by a homology search. The corresponding phylogenetic tree indicated that 14 TaBOR genes were separately classified into subfamilies of TaBOR1, TaBOR3, and TaBOR4. All TaBOR genes had 12–14 extrons and 11–13 introns. Most TaBOR proteins contained 10 conserved motifs, and motifs 1, 2, 3, 4, and 6 constituted the conserved bicarbonate (HCO3–) domain. Fourteen TaBOR genes were mapped on 13 chromosomes mainly distributed in the first, third, fifth, and seventh homologous groups. The promoters of TaBOR genes consisted of phytohormones, light responses, and stress-related cis-elements. GO analysis indicated that TaBOR genes were enriched in terms of transmembrane transport and ion homeostasis. TaBOR genes showed diverse expression profiles in different tissues. The members of the TaBOR1 subfamily showed high expression in grains, leaves, roots, stems, and spikes, but members of the TaBOR4 subfamily were highly expressed only in spikes and grains. RT–qPCR indicated that TaBOR1-5A, TaBOR1-5B, and TaBOR1-5D were induced by low B concentrations and had much higher expression in roots than in shoots. TaBOR3-3A, TaBOR3-3B, TaBOR3-3D, TaBOR4-1A, TaBOR4-1B, TaBOR4-1D, and TaBOR3-4B were induced by low and high B concentrations and had high expression in roots and shoots. TaBOR3-4D and TaBOR3-7B were upregulated by low and high B concentrations, respectively, but had expression only in roots. Our results provide basic information on the TaBOR family, which is beneficial for elucidating the functions of TaBOR genes to overcome the problem of B deficiency. |
| first_indexed | 2024-04-13T23:19:31Z |
| format | Article |
| id | doaj.art-6dacfa1d0ddb4ee19238ae2c450b9733 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T23:19:31Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6dacfa1d0ddb4ee19238ae2c450b97332022-12-22T02:25:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.997915997915Molecular characterization of the genome-wide BOR transporter family and their responses to boron conditions in common wheat (Triticum aestivum L.)Yuquan Wang0Yuquan Wang1Zhipeng Niu2Zhipeng Niu3Xigui Hu4Xigui Hu5Xiaojun Wu6Xiaojun Wu7Zijun Yang8Zijun Yang9Chenyan Hao10Chenyan Hao11Mengxue Zhou12Mengxue Zhou13Shumin Yang14Shumin Yang15Na Dong16Na Dong17Mingjiu Liu18Mingjiu Liu19Zhengang Ru20Zhengang Ru21Henan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaHenan Key Laboratory of Hybrid Wheat, Xinxiang, Henan, ChinaSchool of Life Sciences and Technology, Henan Institute of Science and Technology, Xinxiang, Henan, ChinaBoron (B) deficiency is an agricultural problem that causes significant yield losses in many countries. B transporters (BORs) are responsible for B uptake and distribution and play important roles in yield formation. A comprehensive analysis of the BOR family members in common wheat is still lacking. In the present study, to clarify the molecular characterization and response to B status, genome-wide TaBOR genes and expression patterns were investigated. Fourteen TaBOR genes were identified in common wheat by a homology search. The corresponding phylogenetic tree indicated that 14 TaBOR genes were separately classified into subfamilies of TaBOR1, TaBOR3, and TaBOR4. All TaBOR genes had 12–14 extrons and 11–13 introns. Most TaBOR proteins contained 10 conserved motifs, and motifs 1, 2, 3, 4, and 6 constituted the conserved bicarbonate (HCO3–) domain. Fourteen TaBOR genes were mapped on 13 chromosomes mainly distributed in the first, third, fifth, and seventh homologous groups. The promoters of TaBOR genes consisted of phytohormones, light responses, and stress-related cis-elements. GO analysis indicated that TaBOR genes were enriched in terms of transmembrane transport and ion homeostasis. TaBOR genes showed diverse expression profiles in different tissues. The members of the TaBOR1 subfamily showed high expression in grains, leaves, roots, stems, and spikes, but members of the TaBOR4 subfamily were highly expressed only in spikes and grains. RT–qPCR indicated that TaBOR1-5A, TaBOR1-5B, and TaBOR1-5D were induced by low B concentrations and had much higher expression in roots than in shoots. TaBOR3-3A, TaBOR3-3B, TaBOR3-3D, TaBOR4-1A, TaBOR4-1B, TaBOR4-1D, and TaBOR3-4B were induced by low and high B concentrations and had high expression in roots and shoots. TaBOR3-4D and TaBOR3-7B were upregulated by low and high B concentrations, respectively, but had expression only in roots. Our results provide basic information on the TaBOR family, which is beneficial for elucidating the functions of TaBOR genes to overcome the problem of B deficiency.https://www.frontiersin.org/articles/10.3389/fpls.2022.997915/fullboronTaBOR genegene familyexpression patternB deficiency |
| spellingShingle | Yuquan Wang Yuquan Wang Zhipeng Niu Zhipeng Niu Xigui Hu Xigui Hu Xiaojun Wu Xiaojun Wu Zijun Yang Zijun Yang Chenyan Hao Chenyan Hao Mengxue Zhou Mengxue Zhou Shumin Yang Shumin Yang Na Dong Na Dong Mingjiu Liu Mingjiu Liu Zhengang Ru Zhengang Ru Molecular characterization of the genome-wide BOR transporter family and their responses to boron conditions in common wheat (Triticum aestivum L.) Frontiers in Plant Science boron TaBOR gene gene family expression pattern B deficiency |
| title | Molecular characterization of the genome-wide BOR transporter family and their responses to boron conditions in common wheat (Triticum aestivum L.) |
| title_full | Molecular characterization of the genome-wide BOR transporter family and their responses to boron conditions in common wheat (Triticum aestivum L.) |
| title_fullStr | Molecular characterization of the genome-wide BOR transporter family and their responses to boron conditions in common wheat (Triticum aestivum L.) |
| title_full_unstemmed | Molecular characterization of the genome-wide BOR transporter family and their responses to boron conditions in common wheat (Triticum aestivum L.) |
| title_short | Molecular characterization of the genome-wide BOR transporter family and their responses to boron conditions in common wheat (Triticum aestivum L.) |
| title_sort | molecular characterization of the genome wide bor transporter family and their responses to boron conditions in common wheat triticum aestivum l |
| topic | boron TaBOR gene gene family expression pattern B deficiency |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.997915/full |
| work_keys_str_mv | AT yuquanwang molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT yuquanwang molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT zhipengniu molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT zhipengniu molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT xiguihu molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT xiguihu molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT xiaojunwu molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT xiaojunwu molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT zijunyang molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT zijunyang molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT chenyanhao molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT chenyanhao molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT mengxuezhou molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT mengxuezhou molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT shuminyang molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT shuminyang molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT nadong molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT nadong molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT mingjiuliu molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT mingjiuliu molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT zhengangru molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml AT zhengangru molecularcharacterizationofthegenomewidebortransporterfamilyandtheirresponsestoboronconditionsincommonwheattriticumaestivuml |