The chalcone butein from <it>Rhus verniciflua </it>Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblasts
<p>Abstract</p> <p>Background</p> <p>Butein (3,4,2',4'-tetrahydroxychalone), a plant polyphenol, is a major biologically active component of the stems of <it>Rhus verniciflua </it>Stokes. It has long been used as a food additive in Korea and as an...
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| Format: | Article |
| Language: | English |
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BMC
2005-03-01
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| Series: | BMC Complementary and Alternative Medicine |
| Online Access: | http://www.biomedcentral.com/1472-6882/5/5 |
| _version_ | 1818336484927733760 |
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| author | Tan Jenny Samoszuk Michael Chorn Guillaume |
| author_facet | Tan Jenny Samoszuk Michael Chorn Guillaume |
| author_sort | Tan Jenny |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Butein (3,4,2',4'-tetrahydroxychalone), a plant polyphenol, is a major biologically active component of the stems of <it>Rhus verniciflua </it>Stokes. It has long been used as a food additive in Korea and as an herbal medicine throughout Asia. Recently, butein has been shown to suppress the functions of fibroblasts. Because fibroblasts are believed to play an important role in promoting the growth of breast cancer cells, we investigated the ability of butein to inhibit the clonogenic growth of small numbers of breast cancer cells co-cultured with fibroblasts in vitro.</p> <p>Methods</p> <p>We first measured the clonogenic growth of small numbers of the UACC-812 human breast cancer cell line co-cultured on monolayers of serum-activated, human fibroblasts in the presence of butein (2 μg/mL) or various other modulators of fibroblast function (troglitazone-1 μg/mL; GW9662-1 μM; meloxican-1 μM; and 3,4 dehydroproline-10 μg/mL). In a subsequent experiment, we measured the dose-response effect on the clonogenic growth of UACC-812 breast cancer cells by pre-incubating the fibroblasts with varying concentrations of butein (10 μg/ml-1.25 μg/mL). Finally, we measured the clonogenic growth of primary breast cancer cells obtained from 5 clinical specimens with normal fibroblasts and with fibroblasts that had been pre-treated with a fixed dose of butein (2.5 μg/mL).</p> <p>Results</p> <p>Of the five modulators of fibroblast function that we tested, butein was by far the most potent inhibitor of clonogenic growth of UACC-812 breast cancer cells co-cultured with fibroblasts. Pre-treatment of fibroblasts with concentrations of butein as low as 2.5 μg/mL nearly abolished subsequent clonogenic growth of UACC-812 breast cancer cells co-cultured with the fibroblasts. A similar dose of butein had no effect on the clonogenic growth of breast cancer cells cultured in the absence of fibroblasts. Significantly, clonogenic growth of the primary breast cancer cells was also significantly reduced or abolished when the tumor cells were co-cultured with fibroblasts that had been pre-treated with a fixed dose of butein.</p> <p>Conclusion</p> <p>We conclude that fibroblasts pre-treated with non-toxic doses of butein (a natural herbal compound) no longer support the clonogenic growth of small numbers of primary breast cancer cells seeded into co-cultures. These results suggest that interference with the interaction between fibroblasts and breast cancer cells by the natural herbal compound, butein, should be further investigated as a novel experimental approach for possibly suppressing the growth of micrometastases of breast cancer.</p> |
| first_indexed | 2024-12-13T14:40:04Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-13T14:40:04Z |
| publishDate | 2005-03-01 |
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| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-6dad40978638446e980e76f49c90ca5e2022-12-21T23:41:38ZengBMCBMC Complementary and Alternative Medicine1472-68822005-03-0151510.1186/1472-6882-5-5The chalcone butein from <it>Rhus verniciflua </it>Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblastsTan JennySamoszuk MichaelChorn Guillaume<p>Abstract</p> <p>Background</p> <p>Butein (3,4,2',4'-tetrahydroxychalone), a plant polyphenol, is a major biologically active component of the stems of <it>Rhus verniciflua </it>Stokes. It has long been used as a food additive in Korea and as an herbal medicine throughout Asia. Recently, butein has been shown to suppress the functions of fibroblasts. Because fibroblasts are believed to play an important role in promoting the growth of breast cancer cells, we investigated the ability of butein to inhibit the clonogenic growth of small numbers of breast cancer cells co-cultured with fibroblasts in vitro.</p> <p>Methods</p> <p>We first measured the clonogenic growth of small numbers of the UACC-812 human breast cancer cell line co-cultured on monolayers of serum-activated, human fibroblasts in the presence of butein (2 μg/mL) or various other modulators of fibroblast function (troglitazone-1 μg/mL; GW9662-1 μM; meloxican-1 μM; and 3,4 dehydroproline-10 μg/mL). In a subsequent experiment, we measured the dose-response effect on the clonogenic growth of UACC-812 breast cancer cells by pre-incubating the fibroblasts with varying concentrations of butein (10 μg/ml-1.25 μg/mL). Finally, we measured the clonogenic growth of primary breast cancer cells obtained from 5 clinical specimens with normal fibroblasts and with fibroblasts that had been pre-treated with a fixed dose of butein (2.5 μg/mL).</p> <p>Results</p> <p>Of the five modulators of fibroblast function that we tested, butein was by far the most potent inhibitor of clonogenic growth of UACC-812 breast cancer cells co-cultured with fibroblasts. Pre-treatment of fibroblasts with concentrations of butein as low as 2.5 μg/mL nearly abolished subsequent clonogenic growth of UACC-812 breast cancer cells co-cultured with the fibroblasts. A similar dose of butein had no effect on the clonogenic growth of breast cancer cells cultured in the absence of fibroblasts. Significantly, clonogenic growth of the primary breast cancer cells was also significantly reduced or abolished when the tumor cells were co-cultured with fibroblasts that had been pre-treated with a fixed dose of butein.</p> <p>Conclusion</p> <p>We conclude that fibroblasts pre-treated with non-toxic doses of butein (a natural herbal compound) no longer support the clonogenic growth of small numbers of primary breast cancer cells seeded into co-cultures. These results suggest that interference with the interaction between fibroblasts and breast cancer cells by the natural herbal compound, butein, should be further investigated as a novel experimental approach for possibly suppressing the growth of micrometastases of breast cancer.</p>http://www.biomedcentral.com/1472-6882/5/5 |
| spellingShingle | Tan Jenny Samoszuk Michael Chorn Guillaume The chalcone butein from <it>Rhus verniciflua </it>Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblasts BMC Complementary and Alternative Medicine |
| title | The chalcone butein from <it>Rhus verniciflua </it>Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblasts |
| title_full | The chalcone butein from <it>Rhus verniciflua </it>Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblasts |
| title_fullStr | The chalcone butein from <it>Rhus verniciflua </it>Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblasts |
| title_full_unstemmed | The chalcone butein from <it>Rhus verniciflua </it>Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblasts |
| title_short | The chalcone butein from <it>Rhus verniciflua </it>Stokes inhibits clonogenic growth of human breast cancer cells co-cultured with fibroblasts |
| title_sort | chalcone butein from it rhus verniciflua it stokes inhibits clonogenic growth of human breast cancer cells co cultured with fibroblasts |
| url | http://www.biomedcentral.com/1472-6882/5/5 |
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