Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation
Multi-class chemical contaminants, such as pesticides and mycotoxins, are recognized as the major risk factors in agro products. It is thus necessary to develop rapid and simple sensing methods to fulfill the on-site monitoring of multi-class chemical contaminants with different physicochemical prop...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/1/56 |
| _version_ | 1811186223807463424 |
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| author | Du Wang Jianguo Zhu Zhaowei Zhang Qi Zhang Wen Zhang Li Yu Jun Jiang Xiaomei Chen Xuefang Wang Peiwu Li |
| author_facet | Du Wang Jianguo Zhu Zhaowei Zhang Qi Zhang Wen Zhang Li Yu Jun Jiang Xiaomei Chen Xuefang Wang Peiwu Li |
| author_sort | Du Wang |
| collection | DOAJ |
| description | Multi-class chemical contaminants, such as pesticides and mycotoxins, are recognized as the major risk factors in agro products. It is thus necessary to develop rapid and simple sensing methods to fulfill the on-site monitoring of multi-class chemical contaminants with different physicochemical properties. Herein, a lateral flow immunoassay via time-resolved fluorescence was developed for the rapid, on-site, simultaneous, and quantitative sensing aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), zearalenone (ZEA), and chlorothalonil (CTN) in maize and peanut. The sample preparation was optimized to a single step, combining the grinding and extraction. Under optimal conditions, the sensing method lowered the limits of detection (LOD) to 0.16, 0.52, and 1.21 µg/kg in maize and 0.18, 0.57, and 1.47 µg/kg in peanut with an analytical range of 0.48⁻20, 1.56⁻200, and 3.63⁻300 µg/kg for AFB<sub>1</sub>, ZEA and CTN, respectively. The protocol could be completed within 15 min, including sample preparation and lateral flow immunoassay. The recovery range was 83.24⁻110.80%. An excellent correlation was observed between this approach and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for mycotoxins and gas chromatography-tandem mass spectrometry (GC-MS/MS) for pesticide in maize and peanut. This work could be applied in on-site multi-class sensing for food safety. |
| first_indexed | 2024-04-11T13:43:01Z |
| format | Article |
| id | doaj.art-6dad490c47a84051b6666269f026971a |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T13:43:01Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-6dad490c47a84051b6666269f026971a2022-12-22T04:21:12ZengMDPI AGToxins2072-66512019-01-011115610.3390/toxins11010056toxins11010056Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample PreparationDu Wang0Jianguo Zhu1Zhaowei Zhang2Qi Zhang3Wen Zhang4Li Yu5Jun Jiang6Xiaomei Chen7Xuefang Wang8Peiwu Li9Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaMulti-class chemical contaminants, such as pesticides and mycotoxins, are recognized as the major risk factors in agro products. It is thus necessary to develop rapid and simple sensing methods to fulfill the on-site monitoring of multi-class chemical contaminants with different physicochemical properties. Herein, a lateral flow immunoassay via time-resolved fluorescence was developed for the rapid, on-site, simultaneous, and quantitative sensing aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), zearalenone (ZEA), and chlorothalonil (CTN) in maize and peanut. The sample preparation was optimized to a single step, combining the grinding and extraction. Under optimal conditions, the sensing method lowered the limits of detection (LOD) to 0.16, 0.52, and 1.21 µg/kg in maize and 0.18, 0.57, and 1.47 µg/kg in peanut with an analytical range of 0.48⁻20, 1.56⁻200, and 3.63⁻300 µg/kg for AFB<sub>1</sub>, ZEA and CTN, respectively. The protocol could be completed within 15 min, including sample preparation and lateral flow immunoassay. The recovery range was 83.24⁻110.80%. An excellent correlation was observed between this approach and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for mycotoxins and gas chromatography-tandem mass spectrometry (GC-MS/MS) for pesticide in maize and peanut. This work could be applied in on-site multi-class sensing for food safety.https://www.mdpi.com/2072-6651/11/1/56simultaneous lateral flow immunoassaymulti-class chemical contaminantsaflatoxin B<sub>1</sub>zearalenonechlorothalonilmaize and peanut |
| spellingShingle | Du Wang Jianguo Zhu Zhaowei Zhang Qi Zhang Wen Zhang Li Yu Jun Jiang Xiaomei Chen Xuefang Wang Peiwu Li Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation Toxins simultaneous lateral flow immunoassay multi-class chemical contaminants aflatoxin B<sub>1</sub> zearalenone chlorothalonil maize and peanut |
| title | Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation |
| title_full | Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation |
| title_fullStr | Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation |
| title_full_unstemmed | Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation |
| title_short | Simultaneous Lateral Flow Immunoassay for Multi-Class Chemical Contaminants in Maize and Peanut with One-Stop Sample Preparation |
| title_sort | simultaneous lateral flow immunoassay for multi class chemical contaminants in maize and peanut with one stop sample preparation |
| topic | simultaneous lateral flow immunoassay multi-class chemical contaminants aflatoxin B<sub>1</sub> zearalenone chlorothalonil maize and peanut |
| url | https://www.mdpi.com/2072-6651/11/1/56 |
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