The Impact of the Molecular Weight of Degradation Products with Silicon from Porous Chitosan–Siloxane Hybrids on Neuronal Cell Behavior
Silicon (Si) is an essential trace element in the human body and it exists in connective tissue as aqueous orthosilicic acid. Porous chitosan–3-glycidoxypropyltrimethoxysilane (GPTMS) hybrids can regenerate nerve tissue and recover sensor and motor functions. However, the structures and roles of the...
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MDPI AG
2023-08-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/15/3272 |
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| author | Yuki Shirosaki Federica Fregnan Luisa Muratori Saki Yasutomi Stefano Geuna Stefania Raimondo |
| author_facet | Yuki Shirosaki Federica Fregnan Luisa Muratori Saki Yasutomi Stefano Geuna Stefania Raimondo |
| author_sort | Yuki Shirosaki |
| collection | DOAJ |
| description | Silicon (Si) is an essential trace element in the human body and it exists in connective tissue as aqueous orthosilicic acid. Porous chitosan–3-glycidoxypropyltrimethoxysilane (GPTMS) hybrids can regenerate nerve tissue and recover sensor and motor functions. However, the structures and roles of the degradation products with Si extracted from the hybrids in nerve regeneration are not clear. In this study, we prepared porous chitosan–GPTMS hybrids with different amounts of GPTMS to amino groups of chitosan (chitosan:GPTMS = 1:0.5 and 1:1 molar ratios). The structures of the degradation products with Si from the hybrids were examined using time-of-flight mass spectrometry, and biological assessments were conducted in order to evaluate their potential use in the preparation of devices for nerve repair. Glial and motor cell lines and ex vivo explants of dorsal root ganglia were used in this study for evaluating their behavior in the presence of the different degradation products with Si. The structure of the degradation products with Si depended on the starting composition. The results showed that glial cell proliferation was lower in the medium with the higher-molecular-weight degradation products with Si. Moreover, motor cell line differentiation and the neurite outgrowth of dorsal root ganglion explants were improved with the lower-molecular-weight degradation products with Si. The results obtained could be useful for designing a new nerve regeneration scaffold including silicon components. |
| first_indexed | 2024-03-11T00:18:26Z |
| format | Article |
| id | doaj.art-6dad8bde377e40639a3938e17b4b1a3b |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T00:18:26Z |
| publishDate | 2023-08-01 |
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| record_format | Article |
| series | Polymers |
| spelling | doaj.art-6dad8bde377e40639a3938e17b4b1a3b2023-11-18T23:28:53ZengMDPI AGPolymers2073-43602023-08-011515327210.3390/polym15153272The Impact of the Molecular Weight of Degradation Products with Silicon from Porous Chitosan–Siloxane Hybrids on Neuronal Cell BehaviorYuki Shirosaki0Federica Fregnan1Luisa Muratori2Saki Yasutomi3Stefano Geuna4Stefania Raimondo5Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu 804-8550, JapanDepartment of Clinical and Biological Sciences and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Regione Gonzole 10, 10043 Orbassano, ItalyDepartment of Clinical and Biological Sciences and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Regione Gonzole 10, 10043 Orbassano, ItalyFaculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu 804-8550, JapanDepartment of Clinical and Biological Sciences and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Regione Gonzole 10, 10043 Orbassano, ItalyDepartment of Clinical and Biological Sciences and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Regione Gonzole 10, 10043 Orbassano, ItalySilicon (Si) is an essential trace element in the human body and it exists in connective tissue as aqueous orthosilicic acid. Porous chitosan–3-glycidoxypropyltrimethoxysilane (GPTMS) hybrids can regenerate nerve tissue and recover sensor and motor functions. However, the structures and roles of the degradation products with Si extracted from the hybrids in nerve regeneration are not clear. In this study, we prepared porous chitosan–GPTMS hybrids with different amounts of GPTMS to amino groups of chitosan (chitosan:GPTMS = 1:0.5 and 1:1 molar ratios). The structures of the degradation products with Si from the hybrids were examined using time-of-flight mass spectrometry, and biological assessments were conducted in order to evaluate their potential use in the preparation of devices for nerve repair. Glial and motor cell lines and ex vivo explants of dorsal root ganglia were used in this study for evaluating their behavior in the presence of the different degradation products with Si. The structure of the degradation products with Si depended on the starting composition. The results showed that glial cell proliferation was lower in the medium with the higher-molecular-weight degradation products with Si. Moreover, motor cell line differentiation and the neurite outgrowth of dorsal root ganglion explants were improved with the lower-molecular-weight degradation products with Si. The results obtained could be useful for designing a new nerve regeneration scaffold including silicon components.https://www.mdpi.com/2073-4360/15/15/3272nerve regenerationorganic–inorganic hybridschitosanSi-containing degradation products |
| spellingShingle | Yuki Shirosaki Federica Fregnan Luisa Muratori Saki Yasutomi Stefano Geuna Stefania Raimondo The Impact of the Molecular Weight of Degradation Products with Silicon from Porous Chitosan–Siloxane Hybrids on Neuronal Cell Behavior Polymers nerve regeneration organic–inorganic hybrids chitosan Si-containing degradation products |
| title | The Impact of the Molecular Weight of Degradation Products with Silicon from Porous Chitosan–Siloxane Hybrids on Neuronal Cell Behavior |
| title_full | The Impact of the Molecular Weight of Degradation Products with Silicon from Porous Chitosan–Siloxane Hybrids on Neuronal Cell Behavior |
| title_fullStr | The Impact of the Molecular Weight of Degradation Products with Silicon from Porous Chitosan–Siloxane Hybrids on Neuronal Cell Behavior |
| title_full_unstemmed | The Impact of the Molecular Weight of Degradation Products with Silicon from Porous Chitosan–Siloxane Hybrids on Neuronal Cell Behavior |
| title_short | The Impact of the Molecular Weight of Degradation Products with Silicon from Porous Chitosan–Siloxane Hybrids on Neuronal Cell Behavior |
| title_sort | impact of the molecular weight of degradation products with silicon from porous chitosan siloxane hybrids on neuronal cell behavior |
| topic | nerve regeneration organic–inorganic hybrids chitosan Si-containing degradation products |
| url | https://www.mdpi.com/2073-4360/15/15/3272 |
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