Supplementation of Selenium Nanoparticles-Loaded Chitosan Improves Production Performance, Intestinal Morphology, and Gut Microflora in Broiler Chickens
The current study aimed to evaluate the efficacy of selenium nanoparticles (SeNPs), combined or loaded with chitosan (COS), in broiler chickens reared under standard management protocols. The parameters under investigation were production performance, organ development, components of the intestinal...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Japan Poultry Science Association
2022-07-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/59/3/59_0210026/_pdf/-char/en |
| _version_ | 1827939073831993344 |
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| author | Imad Khan Hafsa Zaneb Saima Masood Saima Ashraf Hafiz F. Rehman Sajid K. Tahir Habib U. Rehman Adnan Khan Raheela Taj Sadeeq U. Rahman Muqader Shah |
| author_facet | Imad Khan Hafsa Zaneb Saima Masood Saima Ashraf Hafiz F. Rehman Sajid K. Tahir Habib U. Rehman Adnan Khan Raheela Taj Sadeeq U. Rahman Muqader Shah |
| author_sort | Imad Khan |
| collection | DOAJ |
| description | The current study aimed to evaluate the efficacy of selenium nanoparticles (SeNPs), combined or loaded with chitosan (COS), in broiler chickens reared under standard management protocols. The parameters under investigation were production performance, organ development, components of the intestinal barrier, and ileal microbial count. Two hundred and forty day-old chicks were raised in five groups, with each group containing eight replicates (n＝6/replicate). The control group received a basal diet whereas the other four groups received basal diets supplemented with SeNPs (0.5 mg/kg), COS (200 mg/kg), SeNPs+COS (0.5 mg/kg SeNPs + 200 mg/kg COS), and SeNPs-loaded COS (SeNPs-L-COS) (200 mg/kg) respectively. On day 35, two birds/replicate were sampled to collect the viscera under investigation. The results revealed that dietary inclusion of SeNPs-L-COS increased (p<0.05) the body weight gain and improved (p<0.05) feed conversion ratio. Similarly, SeNPs-L-COS supplementation increased (p<0.05) the small intestinal villus surface area as well as the count of acidic goblet cells and intraepithelial lymphocytes when compared with the control group. Whereas the total goblet cell count was higher (p<0.05) in the small intestines of both the SeNPs+COS and SeNPs-L-COS groups. Microbial analysis of ileal contents also revealed an increase (p<0.05) in Lactobacilli species count with a concurrent decrease (p<0.05) in Escherichia coli count in the SeNPs-L-COS group when compared with the COS and control groups. Based on the results of the current trial, we can conclude that supplementation with SeNPs-L-COS is a superior combination for promoting the gut health and performance of broilers. |
| first_indexed | 2024-03-13T08:47:04Z |
| format | Article |
| id | doaj.art-6db1ff7993ec487f8ed42d8e0530210b |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:47:04Z |
| publishDate | 2022-07-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-6db1ff7993ec487f8ed42d8e0530210b2023-05-30T00:22:20ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862022-07-0159327228110.2141/jpsa.0210026jpsaSupplementation of Selenium Nanoparticles-Loaded Chitosan Improves Production Performance, Intestinal Morphology, and Gut Microflora in Broiler ChickensImad Khan0Hafsa Zaneb1Saima Masood2Saima Ashraf3Hafiz F. Rehman4Sajid K. Tahir5Habib U. Rehman6Adnan Khan7Raheela Taj8Sadeeq U. Rahman9Muqader Shah10Department of Anatomy and Histology, University of Veterinary and Animal SciencesDepartment of Anatomy and Histology, University of Veterinary and Animal SciencesDepartment of Anatomy and Histology, University of Veterinary and Animal SciencesDepartment of Anatomy and Histology, University of Veterinary and Animal SciencesDepartment of Anatomy and Histology, University of Veterinary and Animal SciencesDepartment of Physiology, University of Veterinary and Animal SciencesDepartment of Physiology, University of Veterinary and Animal SciencesInstitute of Chemical Sciences, University of PeshawarInstitute of Chemical Sciences, University of PeshawarCollege of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan UniversityCollege of Veterinary Sciences, University of AgricultureThe current study aimed to evaluate the efficacy of selenium nanoparticles (SeNPs), combined or loaded with chitosan (COS), in broiler chickens reared under standard management protocols. The parameters under investigation were production performance, organ development, components of the intestinal barrier, and ileal microbial count. Two hundred and forty day-old chicks were raised in five groups, with each group containing eight replicates (n＝6/replicate). The control group received a basal diet whereas the other four groups received basal diets supplemented with SeNPs (0.5 mg/kg), COS (200 mg/kg), SeNPs+COS (0.5 mg/kg SeNPs + 200 mg/kg COS), and SeNPs-loaded COS (SeNPs-L-COS) (200 mg/kg) respectively. On day 35, two birds/replicate were sampled to collect the viscera under investigation. The results revealed that dietary inclusion of SeNPs-L-COS increased (p<0.05) the body weight gain and improved (p<0.05) feed conversion ratio. Similarly, SeNPs-L-COS supplementation increased (p<0.05) the small intestinal villus surface area as well as the count of acidic goblet cells and intraepithelial lymphocytes when compared with the control group. Whereas the total goblet cell count was higher (p<0.05) in the small intestines of both the SeNPs+COS and SeNPs-L-COS groups. Microbial analysis of ileal contents also revealed an increase (p<0.05) in Lactobacilli species count with a concurrent decrease (p<0.05) in Escherichia coli count in the SeNPs-L-COS group when compared with the COS and control groups. Based on the results of the current trial, we can conclude that supplementation with SeNPs-L-COS is a superior combination for promoting the gut health and performance of broilers.https://www.jstage.jst.go.jp/article/jpsa/59/3/59_0210026/_pdf/-char/engut healthmicrobiotapoultryprebiotictrace minerals |
| spellingShingle | Imad Khan Hafsa Zaneb Saima Masood Saima Ashraf Hafiz F. Rehman Sajid K. Tahir Habib U. Rehman Adnan Khan Raheela Taj Sadeeq U. Rahman Muqader Shah Supplementation of Selenium Nanoparticles-Loaded Chitosan Improves Production Performance, Intestinal Morphology, and Gut Microflora in Broiler Chickens The Journal of Poultry Science gut health microbiota poultry prebiotic trace minerals |
| title | Supplementation of Selenium Nanoparticles-Loaded Chitosan Improves Production Performance, Intestinal Morphology, and Gut Microflora in Broiler Chickens |
| title_full | Supplementation of Selenium Nanoparticles-Loaded Chitosan Improves Production Performance, Intestinal Morphology, and Gut Microflora in Broiler Chickens |
| title_fullStr | Supplementation of Selenium Nanoparticles-Loaded Chitosan Improves Production Performance, Intestinal Morphology, and Gut Microflora in Broiler Chickens |
| title_full_unstemmed | Supplementation of Selenium Nanoparticles-Loaded Chitosan Improves Production Performance, Intestinal Morphology, and Gut Microflora in Broiler Chickens |
| title_short | Supplementation of Selenium Nanoparticles-Loaded Chitosan Improves Production Performance, Intestinal Morphology, and Gut Microflora in Broiler Chickens |
| title_sort | supplementation of selenium nanoparticles loaded chitosan improves production performance intestinal morphology and gut microflora in broiler chickens |
| topic | gut health microbiota poultry prebiotic trace minerals |
| url | https://www.jstage.jst.go.jp/article/jpsa/59/3/59_0210026/_pdf/-char/en |
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